Association between primary open angle glaucoma and genetic polymorphisms GSTM1/GSTT1 in patients from Goiânia Central-West Region of Brazil

Abstract

ABSTRACT. In this study, we evaluated the genotype profile of GSTM1 and GSTT1 polymorphisms in patient carriers of primary open-angle glaucoma in the population of Goiânia, GO, Brazil. This case-control study included 100 Brazilian patients with glaucoma and 53 patients without glaucoma. Blood samples were genotyped for polymorphisms in GST genes using polymerase chain reaction-based methods. Polymorphism frequencies were compared using the c 2 test and odds ratio (α = 0.05). The GSTM1-present genotype was 40% in the glaucoma group and 71.6% in the control group, while the GSTM1 null genotype was 60 and 28.3% in the same groups, respectively. The GSTT1-present genotype was 52% in the primary open-angle glaucoma group and 66% in the control group; the null genotype was 48% in the case group and 34% in the control group. The GSTM1 null Glaucoma and GSTM1/GSTT1 polymorphisms genotype was more frequent in the glaucoma group than in the control group (P = 0.0004; odds ratio = 6.7; 95% confidence interval = 2.7-20.3). The combined GSTM1 null and GSTT1-present genotypes were more frequent in the primary open-angle glaucoma group compared to the control group (P = 0.02; odds ratio = 3.1; 95% confidence interval = 1.2-7.9).

Letter to the Editor: Gluthathion S-transferase M1 and T1 polymorpisms in a group of Romanian glaucoma patients

“…Our results are in agreement with the ones reported by Jansson et al who found no association between GSTM1 null genotypes and POAG in a Swedish population (5,6). Similar results were also communicated for GSTM1 in a Brazilian, Mexican (4) and Iranian (5) populations and for GSTT1 in an Estonian, Turkish, Mexican, Brazilian and Iranian population (5,6).…”

“…Similar results were also communicated for GSTM1 in a Brazilian, Mexican (4) and Iranian (5) populations and for GSTT1 in an Estonian, Turkish, Mexican, Brazilian and Iranian population (5,6). Moreover, recent meta-analyses (7)(8)(9) found no significant association between GSTM1 null and GSTT1 genotypes and POAG in Caucasians.…”

Development of Primary Open-Angle Glaucoma and Deletion Polymorphism of the Glutathione-S-Transferase Genes

“…GST enzymes catalyze the conjugation of reduced glutathione, protect the body against toxic substances, reduce endogenous oxidative stress, and neutralize lipid and nucleic acid oxidation products [16]. It has been determined that the enzymatic activity of transcriptional proteins and GST directly depends on the deletion polymorphism of GSTM1 and GSTT1 genes, which are regulators of second-phase xenobiotics biotransformation enzymes [17,18].…”