Association between single nucleotide polymorphisms and sexual precocity in Nellore heifers

Regatieri, Inaê Cristina; Boligon, Arione Augusti; Costa, Raphael Bermal; Souza, Fernanda Fernandes; Baldi, Fernando; Takada, Luciana; Venturini, G. C.; Camargo, Gregório Miguel Ferreira de; Fernandes, G. A.; Tonhati, Humberto; Oliveira, Henrique Nunes de; Albuquerque, Lúcia Galvão de

doi:10.1016/j.anireprosci.2016.12.009

Cited by 17 publications

(8 citation statements)

References 52 publications

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“…This may be due either to the lack of knowledge regarding the functions of these genes or the existence of non-annotated genes in this region. It is noteworthy that the present study detected association in regions different from that reported by previous studies on the Nelore breed [ 77 – 79 ]. This can be because of herd particularities, such as the extent of LD, allelic frequencies, sample size, and statistical approaches [ 79 ], in addition to the use of haplotypes in the current study.…”

Section: Discussioncontrasting

confidence: 99%

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

et al. 2018

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Zebu cattle (Bos taurus indicus) are highly adapted to tropical regions. However, females reach puberty after taurine heifers, which affects the economic efficiency of beef cattle breeding in the tropical regions. The aims of this study were to establish associations between the haplotype alleles of the bovine genome and age at first calving (AFC) in the Nelore cattle, and to identify the genes and quantitative trait loci (QTL) related to this phenotype. A total of 2,273 Nelore cattle (995 males and 1,278 females) genotyped using the Illumina BovineHD BeadChip were used in the current study. The association analysis included females with valid first calving records as well as open heifers. Linkage disequilibrium (LD) analysis among the markers was performed using blocks of 5, 10, and 15 markers, which were determined by sliding windows shifting one marker at a time. Then, the haplotype block size to be used in the association study was chosen based on the highest r2 average among the SNPs in the block. The five HapAlleles most strongly associated with the trait (top five) were considered as significant associations. The results of the analysis revealed four genomic regions related to AFC, which overlapped with 20 QTL of the reproductive traits reported previously. Furthermore, there were 19 genes related to reproduction in those regions. In conclusion, the use of haplotypes allowed the detection of chromosomal regions associated with AFC in Nelore cattle, and provided the basis for elucidating the mechanisms underlying this trait.

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Section: Discussioncontrasting

confidence: 99%

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

et al. 2018

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“…In Nellore heifers, the APP gene is linked to AFC and early pregnancy, which supports that the regions found in the present study agree with what is found in the literature (Regatieri et al, 2017). Murata and Petersen (2004) stated that the number of APPs may be associated with early diagnosis, and that is information that allows monitoring the health status of a herd.…”

Section: Discussionsupporting

confidence: 91%

Genome-wide association study for reproduction traits in a Brazilian Holstein population

Paulin¹

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Genome-wide association study for reproduction traits in a Brazilian Holstein populationBrazil is in an important position in the world of the dairy productive chain, and the efficiency of the production has been discussed in different fields in this chain, among those, reproductive efficiency. Studies on improvement for better comprehension of the management, dietary intake, sanity, and genetic terms, have been applied to comprehend ways to optimize dairy production and turn it more and more profitable for the producer. Reproductive phenotypic characteristics such as age at first calving (AFC), calving interval (CI), and quantity of inseminations needed to impregnate the cow are parameters for understanding and monitoring the reproductive activity of a herd. The ideal is that the cow is as precocious and has the smaller calving interval as possible. It is known that reproductive characteristics have low heritability, undergoing great environmental influence. In this line, it is necessary to know the genes that are involved in this process to have more and more clarity. With genomic association studies and advances in molecular biology, it is possible to explain the genetic background of these characteristics and with the use of molecular markers, we find regions responsible for the origins of the biological variation that show us these characteristics. Thus, this study aimed to identify genomic regions associated with age at first calving (AFC), to calving interval (CI), in a population of Holstein cattle. To identify regions that possibly alter the phenotype, GWAS was applied to the database of phenotypic data of AFC, IC of 39,336 daughters, and genotype of 555 bulls, in 187 different herds, contemporary groups were formed and analyzed in GWAS. Data underwent quality control and by the end, there were 75,925 SNPs. BLUPF90 family software was used for statistical analysis and known pedigree animal model, with 10 SNPs fixed windows, and explained variance over 1.5%. A total of 34 genes were identified: 24 genes linked to AFC and 10 genes linked to CI. The main genes were APP, PRSS16 e BTN1A1, once linked to the animal immune response to the postpartum intemperance and to the reproductive system recovery. The genes CYYR1, PRSS16 e WEE2 are known to be related to embryogenesis, linking them to the reproductive traits of the animals. Some of the identified genes, in the present study, were associated with reproductive and immunological characteristics of the animals, corroborating the hypothesis of a different genetic expression among the investigated animals, and indicating the need for the development of new studies in this field.

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“…Cánovas et al (2014) analyzed eight tissues of pre-and post-pubertal Brangus heifers by RNA-Seq and found that hypothalamus samples had the greatest increase in the puberty regulatory genes as compared with the other analyzed tissues (pituitary gland, uterus, endometrium, ovary, liver, muscle and adipose tissue). In addition, several studies with Nelore heifers have shown that single nucleotide polymorphisms provide relevant information to help elucidate which genes affect puberty onset (Costa et al, 2015;Regatieri et al, 2017).…”

Section: Figurementioning

confidence: 99%

A combination of nutrition and genetics is able to reduce age at puberty in Nelore heifers to below 18 months

Ferraz

Pires

Santos

et al. 2018

Animal

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Nelore heifers usually begin their reproductive life at ⩾24 months of age mainly due to suboptimal nutritional conditions and genetics. This study aimed to determine the effect of expected progeny difference (EPD) for age at first calving and average daily gain (ADG) on puberty in Nelore (Bos taurus indicus) heifers. A total of 58 weaned heifers (initial BW=174±6 kg; age=9±1 months) were allocated into 28 feedlot pens. Heifers were born from four sires, of which two had low EPD for age at first calving (L; n=33) and two had high EPD for age at first calving (H; n=25). Then, heifers of each EPD were randomly assigned to high ADG (HG; 0.7 kg) or low ADG (LG; 0.3 kg), resulting in four treatments: heifers from L sires were submitted to either HG (LHG; n=17) or LG (LLG; n=16), and heifers from H sires were submitted to either HG (HHG; n=12), or LG (HLG; n=13). The HG heifers were fed a 75% grain diet, whereas the LG heifers received 93% of forage in their diet. Blood samples were collected at 9, 14, 18, 24 and 28 months of age for IGF1 and leptin determination. There was a treatment effect (P<0.01) on the proportion of heifers that attained puberty by 18 (62%, 0%, 0% and 0%), 24 (100%, 6%, 54% and 0%) or 36 (100%, 100%, 100% and 38%) months of age for LHG, LLG, HHG and HLG treatments, respectively. In addition, mean age at puberty was different across treatments (P<0.01). Heifers from the LHG achieved puberty at the earliest age when compared with cohorts from other treatments (18.1, 28.9, 23.9 and 34.5 months for LHG, LLG, HHG and HLG, respectively). Serum IGF1 concentrations were higher for L heifers compared with H cohorts at 9, 14, 18, 24 and 28 months of age (P<0.01; treatment×age interaction), whereas circulating leptin concentrations were higher (P<0.01; age effect) as heifers became older, regardless of the treatments. In conclusion, only Nelore heifers with favorable genetic merit for age at first calving were able to attain puberty by 18 months of age. In heifers with unfavorable genetic merit for age at first calving, supplementary feeding to achieve high ADG was unable to shift the age at puberty below 24 months.

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Association between single nucleotide polymorphisms and sexual precocity in Nellore heifers

Cited by 17 publications

References 52 publications

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

Genome-wide association study for reproduction traits in a Brazilian Holstein population

A combination of nutrition and genetics is able to reduce age at puberty in Nelore heifers to below 18 months

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