Association between the prevalence of infestation by Rhipicephalus sanguineus and Ctenocephalides felis felis and the presence of anti-Leishmania antibodies: A case–control study in dogs from a Brazilian endemic area

Paz, Gustavo Fontes; Ribeiro, Múcio Flávio Barbosa; Magalhães, D.F.; Sathler, Kelly P.B.; Morais, Maria Helena Franco; Fiúza, Vanessa O.P.; Brandão, Silvana Tecles; Werneck, Guilherme Loureiro; Fortes-Dias, Consuelo Latorre; Dias, Edelberto Santos

doi:10.1016/j.prevetmed.2010.08.006

Cited by 32 publications

(26 citation statements)

References 17 publications

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“…A presença de outros animais no imóvel pode representar risco diferenciado para o homem (Borges et al, 2009), e estudos vêm sendo feitos com outros possíveis reservatórios e vetores incriminados na transmissão da doença Paz et al, 2010).…”

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Distribuição temporal e espacial da leishmaniose visceral em humanos e cães em Belo Horizonte-MG, 1993 a 2007

Lopes¹,

Magalhães²,

Silva³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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Distribuição temporal e espacial da leishmaniose visceral em humanos e cães em Belo Horizonte-MG, 1993 a 2007

Lopes¹,

Magalhães²,

Silva³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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“…However, entomological studies carried out in different Brazilian municipalities did not confirm the presence of this phlebotomine in areas endemic to L. chagasi (DANTAS-TORRES, 2006). Thus, the possible existence of a new vector has been suggested in the epidemiology of this parasitosis (PAZ, 2010a).…”

Section: Resultsmentioning

confidence: 91%

“…Similarly, fleas have been reported as vectors of Leishmania spp. LINARDI, 2007;PAZ, 2010a). However, the detection of Leishmania spp.…”

Section: Resultsmentioning

confidence: 99%

Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

Coelho

Bresciani

2013

Rev. Bras. Parasitol. Vet.

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Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmania spp. in dipterous insects of the species T. importunus.Keywords: Dogs, canine visceral leishmaniasis, horse fly, PCR, protozoan, vectors. ResumoA leishmaniose é uma importante zoonose, de caráter crônico, causada por protozoários do gênero Leishmania spp. Esta protozoose tem como principal vetor os flebotomíneos, sendo que, no Brasil, o Lutzomyia longipalpis é a principal espécie incriminada na transmissão da leishmaniose Visceral Americana. A presença do ácido desoxirribonucleico (DNA) do parasito em ectoparasitos, como carrapatos e pulgas, tem gerado especulações quanto a existência de novos vetores no ciclo da leishmaniose. Foi objetivo deste estudo relatar a detecção molecular de Leishmania spp. em uma mutuca da espécie Tabanus importunus que parasitava um cão oligossintomático infectado por Leishmania spp. A análise molecular amplificou o DNA do protozoário na cabeça, na região torácica e no abdomen do tabanídeo, resultando como positivo para complexo Leishmania. Este é o primeiro relato da presença de DNA de Leishmania spp. em insetos dipteros da espécie T. importunus.

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“…Akdeniz ül-kelerinde yaygın olarak görülen zoonotik yolla bulaşmada, paraziti taşıyan dişi kum sinekleri tarafından enfekte edilen köpekler hastalığın asıl rezervuarıdırlar (20 Informed Consent: Informed consent was not obtained due to not having a specific group of patients for this study.…”

Section: Discussionunclassified

“…Son yıllarda yapılan çalışmalarda L. infantum'un köpeklere naklinde Rh. sanguineus kenesinin de rol oynadığı bildirilmektedir (18)(19)(20)(21)(22). Yapılan bir çalışmada L. infantum'un Rh.…”

Section: Introductionunclassified

Determination the Role of Rhipicephalus sanguineus for Transmission of Leishmania major to Reservoir Animals

Bilgiç

Bakırcı²,

Köse³

et al. 2017

TurkiyeParazitolDerg

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üzerinde beslenmesini takiben, doymuş larvalar ve uygun koşullarda gömlek değiştiren larvalardan elde edilen aç nimflerden oluşan toplam 65 kene havuzu oluşturulmuştur. Bu havuzlar polimeraz zincir reaksiyonu (PZR) ve gerçek zamanlı PZR (RT-PCR) testi ile L. major yönünden test edilmiştir. Bulgular: Enfekte edilerek, üzerine Rh. sanguineus larvası konulan gerbil, tüm kenelerin düşmesini takiben uyutulmuş ve nekropsisi gerçekleştirilmiştir. Yapılan incelemelerde, gerbilin tüm iç organ ve dokularında amastigotlara rastlanmıştır. Rh. sanguineus doymuş larvalarının beslenmeleri esnasında paraziti alıp almadıkları ve bir sonraki nimf aşamasına aktarıp aktarmadıklarının test edilmesi amacıyla yapılan PZR ve RT-PCR sonucunda kene havuzlarının hiçbirisinde L. major'a rastlanılmamıştır. Sonuç: Çalışma neticesinde her ne kadar kenelerde etkenler tespit edilemese de, leishmaniasis ile ilişkili deneysel çalışmalarda asıl rezervuar olan köpeklerin kullanılması vektörlük potansiyeli yüksek olan insekt ve akarlar için daha net sonuçlar verebilecektir. Anahtar Kelimeler: Gerbil, Leishmania major, nakil, Rhipicephalus sanguineus Geliş Tarihi: 24.05.2016Kabul Tarihi: 06.10.2016 ABSTRACTObjective: This study aimed to explore the role of Rhipicephalus sanguineus in the transmission of Leishmania major, the etiological agent of zoonotic cutaneous leishmaniasis. Methods: Ten gerbils (Meriones unguiculatus) were infected with promastigotes of L. major, and 10 gerbils were maintained as controls. In a controlled environment, 2000 R. sanguineus larvae were fed to two gerbils. Following feeding to gerbils, 65 tick pools were prepared from the engorged larvae and molted unfed nymphs. These pools were tested for the presence of L. major using polymerase chain reaction and real time (RT) PCR. Results: One of the infected gerbil was anesthetized and necropsied following the dropping of all fed larvae. Following the examination, amastigotes were detected in all organs and tissues. PCR and RT-PCR were performed to test whether the engorged R. sanguineus larvae successfully took the parasite while feeding and was able to transmit it to the next nymphal stage; however, none of the tick pools were found to be positive for L. major. Conclusion: Although L.major was not detected in ticks that fed on gerbils, using dogs in experimental studies related to leishmaniasis will give clearer results in terms of detecting the potential role of insects and acars.

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Association between the prevalence of infestation by Rhipicephalus sanguineus and Ctenocephalides felis felis and the presence of anti-Leishmania antibodies: A case–control study in dogs from a Brazilian endemic area

Cited by 32 publications

References 17 publications

Distribuição temporal e espacial da leishmaniose visceral em humanos e cães em Belo Horizonte-MG, 1993 a 2007

Distribuição temporal e espacial da leishmaniose visceral em humanos e cães em Belo Horizonte-MG, 1993 a 2007

Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

Determination the Role of Rhipicephalus sanguineus for Transmission of Leishmania major to Reservoir Animals

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