Association between the vaginal microbiome and high-risk human papillomavirus infection in pregnant Chinese women

Chen, Yulian; Hong, Zubei; Wang, Wenjing; Gu, Liyi; Gao, Hua; Qiu, Lihua; Di, Wen

doi:10.1186/s12879-019-4279-6

Cited by 55 publications

(55 citation statements)

References 52 publications

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“…Although there were no signi cant relative abundance differences of the top 10 bacterial genus, which totally account for 96.25% microbe amount of the whole community, between pregnant and non-pregnant women, we observed the decreased microbial richness measured by PD_whole tree alpha diversity in pregnant than in nonpregnant women and in normal than in infected women. This result are contrary to recently reported by Yulian Chen,et al [11] in that they found both pregnancy and HPV infection can increase vaginal bacterial microbial richness and diversity. Although we consider the reason is they measured the microbial richness by Chao and Shannon index other than we used PD_whole tree that focus on the phylogenic a nity between each microbe, there must exist underlying factors that shield the real association of taxonomic diversity to pregnancy.…”

Section: Discussioncontrasting

confidence: 99%

Reproductive Tract Microbiota of Women in Childbearing Age Shifts Upon Type of Gynecological Infections and Phase in Physiological Cycle

Gao

Cheng

Xia

et al. 2020

Preprint

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Background: The association of the normal physiological cycle to the structural pattern of microbiota in reproductive tract of women at reproductive age has not been extensively explored. This study was undertaken to determine whether the vaginal microbes of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively.Results: This was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Samples of vaginal swabs were collected and stored at -70℃ until assayed. The V3-V4 regions of 16S rRNA genes were amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was different between women with different urogenital tract infections, childbearing history and menstrual cycle. We showed that the vaginal microbial richness(Alpha diversity measured by PD_whole tree) was decreased in normal women(without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of uninfected and non-pregnant. The top 10 genus relative abundance, especially that Lactobacillus was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis. Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. It is proposed that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although this comparison lacks statistical significance.Conclusions: The relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age, characterized with 16S rRNA gene sequence and QIIME based analysis, were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The compositional ratio of the main lactobacillus species may shift depending on the normal physiological cycle and reproductive tract infections.

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Section: Discussioncontrasting

confidence: 99%

Reproductive Tract Microbiota of Women in Childbearing Age Shifts Upon Type of Gynecological Infections and Phase in Physiological Cycle

Gao

Cheng

Xia

et al. 2020

Preprint

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“…All enrolled women were preliminarily screened by both an HPV genotyping test and the ThinPrep cytology test (TCT), using a commercial HPV genotyping kit as previously described [25]. TCT results were categorized according to Bethesda System criteria [26].…”

Section: Study Population and Sample Collectionmentioning

confidence: 99%

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

Chen

Qiu

Wang

et al. 2020

Preprint

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Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n=78), women with low-grade squamous intraepithelial lesions (LSIL, n=51), women with high-grade squamous intraepithelial lesions (HSIL, n=23), women with invasive cervical cancer (Cancer, n=9) and healthy women without HPV infection (Normal, n=68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.Conclusions: This work interpreted the differential vaginal bacteria under HPV infection and various precancerous or cancerous lesions in a Chinese cohort. We distinguished the specific microbes and the vaginal bacterial structure that were related with the progression of CINs severity in Chinese women.

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“…A Chinese study identified several microbial genera in hrHPV-infected women (Bifidobacterium, Bacillus, Megasphaera, Sneathia, Prevotella, Gardnerella, Fastidiosipila, and Dialister), while another set (Bifidobacterium, Megasphaera, Bacillus, Acidovorax, Oceanobacillus, and Lactococcus) in hrHPVinfected pregnant women. In pregnancy, this study showed an association between a more diverse cervical microbiota and HPV (125). In addition, several genera and species were associated to HPV positivity (Ureaplasma parvum), HPV negativity (Brochothrix, Diplorickettsia, Ezakiella, Faecalibacterium, and Fusobacterium), likelihood of reinfection (Actinomyces) or persistence (Prevotella, Dialister, and Lachnospiraceae) (126).…”

Section: Preconceptional Period (Genital Tract Infections)mentioning

confidence: 63%

The Interplay Between Reproductive Tract Microbiota and Immunological System in Human Reproduction

et al. 2020

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In the last decade, the microbiota, i.e., combined populations of microorganisms living inside and on the surface of the human body, has increasingly attracted attention of researchers in the medical field. Indeed, since the completion of the Human Microbiome Project, insight and interest in the role of microbiota in health and disease, also through study of its combined genomes, the microbiome, has been steadily expanding. One less explored field of microbiome research has been the female reproductive tract. Research mainly from the past decade suggests that microbial communities residing in the reproductive tract represent a large proportion of the female microbial network and appear to be involved in reproductive failure and pregnancy complications. Microbiome research is facing technological and methodological challenges, as detection techniques and analysis methods are far from being standardized. A further hurdle is understanding the complex host-microbiota interaction and the confounding effect of a multitude of constitutional and environmental factors. A key regulator of this interaction is the maternal immune system that, during the peri-conceptional stage and even more so during pregnancy, undergoes considerable modulation. This review aims to summarize the current literature on reproductive tract microbiota describing the composition of microbiota in different anatomical locations (vagina, cervix, endometrium, and placenta). We also discuss putative mechanisms of interaction between such microbial communities and various aspects of the immune system, with a focus on the characteristic immunological changes during normal pregnancy. Furthermore, we discuss how abnormal microbiota composition, "dysbiosis," is linked to a spectrum of clinical disorders related to the female reproductive system and how the maternal immune system is involved. Finally, based on the data presented in this review, the future perspectives in diagnostic approaches, research directions and therapeutic opportunities are explored.

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Association between the vaginal microbiome and high-risk human papillomavirus infection in pregnant Chinese women

Cited by 55 publications

References 52 publications

Reproductive Tract Microbiota of Women in Childbearing Age Shifts Upon Type of Gynecological Infections and Phase in Physiological Cycle

Reproductive Tract Microbiota of Women in Childbearing Age Shifts Upon Type of Gynecological Infections and Phase in Physiological Cycle

Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort

The Interplay Between Reproductive Tract Microbiota and Immunological System in Human Reproduction

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