Association between thrombomodulin and high mobility group box 1 in sepsis patients

Rodrigues, Adriana Teixeira; Rodrigues, Julia Teixeira; Rodrigues, Carolina Teixeira; Volpe, Caroline Maria Oliveira; Rocha-Silva, Fabiana; Nogueira-Machado, José Augusto; Alberti, Luiz Ronaldo

doi:10.5492/wjccm.v9.i4.63

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…High mobility group box 1 (HMGB1) is critical in the pathogenesis of sepsis. [7][8][9] HMGB1 is stored in the nucleus. Bacteria lipopolysaccharide (LPS) can cause HMGB1 acetylation, resulting in the localization of the protein to the cytosol.…”

Section: Introductionmentioning

confidence: 99%

Emodin alleviates sepsis‐mediated lung injury via inhibition and reduction of NF‐kB and HMGB1 pathways mediated by SIRT1

Liu

Wei

2021

The Kaohsiung J of Med Scie

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Inflammation plays an important role during sepsis, and excessive inflammation can result in organ damage, chronic inflammation, fibrosis, and scarring. The study aimed to investigate the specific mechanism of emodin by constructing in vivo and in vitro septic lung injury models via inhibition and reduction of NF-kB and high mobility group box 1 (HMGB1) pathways. A cecal ligation and puncture (CLP) model was built for adult male Sprague-Dawley rats. Concentrations of TNF-α, IL-1β, and IL-6 in bronchoalveolar lavage fluid were determined using commercially available ELISA kits. Hematoxylin and eosin staining was used for the right lung inferior lobes. Myeloperoxidase (MPO) activity of the lung tissue was detected by using the MPO kit. Murine alveolar epithelial cell line (MLE-12) cells were used for flow cytometry and Western blot to analyze the apoptosis rate and protein expression. Emodin significantly decreased CLP-induced cell apoptosis, upregulated expression of sirtuin 1 (SIRT1), and inhibited p-p65/p65 and HMGB1. In lipopolysaccharide (LPS) treated cell model, emodin treatment markedly decreased LPS-induced release of IL-1, IL-6, and tumor necrosis factor (TNF)-α, inhibited LPS-induced cell apoptosis and suppressed protein levels of P-P65/P65 and HMGB1. However, science of SIRT1 reversed the above effects by treatment of emodin. In summarize, this study found that emodin can alleviate sepsis-induced lung injury in vivo and in vitro through regulation of SIRT1.

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Section: Introductionmentioning

confidence: 99%

Emodin alleviates sepsis‐mediated lung injury via inhibition and reduction of NF‐kB and HMGB1 pathways mediated by SIRT1

Liu

Wei

2021

The Kaohsiung J of Med Scie

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“…15 Research by Rodrigues et al showed that the group of patients with a SOFA score greater than or equal to 12 points had a statistically significantly higher level of sTM than the group with a SOFA score less than 12 points. 25 According to this study, the levels of sTM, IL-6, SOFA score, and APACHE II score in the group that died within 28 days were statistically significantly higher than those in the survival group (p < 0.05). Using ROC analysis, our study showed that the AUC of sTM, IL-6, SOFA score, and APACHE II score in predicting mortality was 0.811, 0.671, 0.816, and 0.705, respectively.…”

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confidence: 53%

Value of Serum Thrombomodulin as a Marker and Predictor in Patients with Sepsis-Associated Acute Kidney Injury

Nguyen¹,

Nguyen-Phan²,

Ton³

et al. 2023

IJGM

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To investigate the serum soluble thrombomodulin (sTM) concentration in patients with sepsis-associated acute kidney injury (AKI) and to determine the value of sTM in predicting AKI and mortality in sepsis patients. Methods: This prospective observational study was conducted on 71 patients diagnosed with sepsis according to Sepsis 3 at the Intensive Care Unit, Hue Central Hospital, Vietnam, from September 2021 to February 2023. Results: Among 71 sepsis patients, there were 38 (53.5%) AKI cases, including 16 (22.5%) cases of stage 1 AKI, 14 (19.7%) cases of stage 2 AKI, 8 (11.3%) cases of stage 3 AKI, 16 (22.5%) cases of renal replacement therapy, 28 (39.4%) cases of septic shock, and 21 (29.6%) cases of mortality within 28 days. The concentrations of lactate and IL-6 in the AKI and mortality groups were statistically significantly greater than those in the non-AKI and survival groups (p < 0.05). The serum sTM concentration was 4.33 ng/mL, the serum sTM level in the AKI group was statistically significantly higher than that in the non-AKI group (sTM [4.71 vs 2.54 ng/mL, p < 0.001]), and the serum sTM level in the mortality group was statistically significantly higher than the survival group (sTM [4.78 vs 3.87 ng/mL, p < 0.001]). The AUC of sTM for predicting AKI was 0.864; the AUCs of sTM, IL-6, SOFA, and APACHE II for predicting mortality were 0.811, 0.671, 0.816, and 0.705, respectively. Conclusion: AKI was a prevalent complication among sepsis patients at the ICU. In the AKI and mortality groups, sTM concentration was statistically significantly higher than that in the non-AKI and survival groups. sTM was the predictor of acute kidney injury and mortality in patients with sepsis.

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“…sTM concentration can be used to track disseminated intravascular coagulation (DIC) and multiple organ dysfunction syndrome (MODS) as well as sepsis severity and the needs of renal replacement therapy ( 13 , 14 ). Additionally, sTM level strongly associated with the severity of organ dysfunction and therefore is a reliable biomarker in early recognition of sepsis patient, especially one with septic shock ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Serum Thrombomodulin Level Can Predict Mortality in Patients With Sepsis?

Nguyen,

Phan,

Hoang

2023

Med Arch

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Background: Thrombomodulin (TM) is a type-1 trans-membrane glycoprotein on endothelial cells which is known to be involved in various biochemical pathways. TM can be detected in biological fluids such as blood and urine under many forms. Soluble thrombomodulin (sTM), consist of various particles of TM, is the predominant agent which is created by enzymatic or chemical catalysis of the whole protein under divergent conditions. TM plays a vital role in protein C system and is crucial in the pathogenesis of Sepsis. Objective: To identify the serum level of soluble thrombomodulin (sTM) in groups of patients: sepsis and septic shock including their survival and fatal in-hospital outcome; and validate the death prediction of serum sTM in patients with sepsis. Methods: This prospective observational study was conducted in 63 patients who were diagnosed with sepsis, septic shock according to Sepsis 3 criteria at the ICU Department of Hue Central Hospital, Vietnam, from 3/2022 to 3/2023. Results: Twenty participants developed septic shock (30.2%), 22 patients complicated with acute renal failure that necessitated renal replacement therapy (34.9%), 30 patients required mechanical ventilation (47.6%), the median length of ICU stay was 8 (3-28) days. Serum level of lactate and creatinine were significantly higher in septic shock group compared with sepsis and survival group (p<0.05). The median sTM level in septic shock group and fatal group were 4.68(3.38-6.46) ng/mL and 4.68 (1.69-6.46) ng/mL, respectively. These results were significantly higher than sepsis group [3.62 (1.51-1.94) ng/mL] and survival group [3.73 (1.51-5.9) ng/mL] (p<0.05). The death predictive power of DIC score, APACHE II score, creatinine, sTM and SOFA presented with AUC values of 0.723, 0.726, 0.777, 0.803 and 0.807, respectively. There were no significant difference of serum level IL-6 and PCT between survival and fatal group. The median DIC score in fatal group was 7 (3-7), which was significantly higher than survival group 4 (2-7) (p= 0.001).

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Association between thrombomodulin and high mobility group box 1 in sepsis patients

Cited by 7 publications

References 50 publications

Emodin alleviates sepsis‐mediated lung injury via inhibition and reduction of NF‐kB and HMGB1 pathways mediated by SIRT1

Emodin alleviates sepsis‐mediated lung injury via inhibition and reduction of NF‐kB and HMGB1 pathways mediated by SIRT1

Value of Serum Thrombomodulin as a Marker and Predictor in Patients with Sepsis-Associated Acute Kidney Injury

Serum Thrombomodulin Level Can Predict Mortality in Patients With Sepsis?

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