Association between β-Lactamase-Encoding bla _OXA-51 Variants and DiversiLab Rep-PCR-Based Typing of Acinetobacter baumannii Isolates

Abstract

bThis study investigated the correlation between bla OXA-51 variants and Acinetobacter baumannii worldwide clonal lineages 1 to 8 (WW1 to -8). The bla OXA-51-like genes of 102 A. baumannii isolates were sequenced. Using DiversiLab repetitive-sequence-based PCR (rep-PCR) typing, 92 of these isolates had previously been assigned to WW1 to -8 and 10 were unclustered. Clustering of DNA sequences was performed using the neighbor-joining method and the Jukes-Cantor phylogenetic correction. bla OXA-51 variants were in good correlation with DiversiLab-defined clonal lineages. Sequence-based typing of bla OXA-51 variants has the potential to be applied for epidemiologic characterization of A. baumannii and to identify worldwide clonal lineages 1 to 8.

Single-Locus-Sequence-Based Typing of bla OXA-51-like Genes for Rapid Assignment of Acinetobacter baumannii Clinical Isolates to International Clonal Lineages

“…Until now, this possibility was tested in parallel only with the PubMLST scheme (12), against which the bla OXA-51-like SBT was shown to identify accurately isolates belonging to the three major epidemic lineages. In another study, the bla OXA-51-like SBT compared with rep-PCR identified international clonal lineages I to III and worldwide clonal lineages 4 to 8 (17). In the current report, the performance of bla OXA-51-like SBT was compared with that of the Pasteur's MLST scheme, which does not exhibit recombination in its target loci in contrast with PubMLST, and also with that of 3-LST, including both the initial multiplex PCR and the sequencing analysis.…”

“…The dendrograms show the amounts of genetic change based on multiple alignments and were generated using phylogeny.fr software (17). The bar at the bottom of each figure shows the amount of genetic change corresponding to the length of each branch.…”

“…The potential of the identification of the specific bla OXA-51-like gene to correctly assign A. baumannii isolates to international clones as single-locus-based typing (SBT) has been scarcely evaluated previously on international collections, in comparison only with PubMLST (12) and rep-PCR (17). In the present study, the performance of this simple, low-cost, and rapid SBT method was evaluated in a large international collection of A.…”

Pneumonia caused by extensive drug-resistant Acinetobacter baumannii among hospitalized patients: genetic relationships, risk factors and mortality

“…Recently, sequencebased typing (SBT) of bla OXA-51-like gene variants was reported to have potential for use in the epidemiological characterization of A. baumannii isolates obtained from various locations in Europe. bla OXA-69 , bla OXA-66 , and bla OXA-71 are the predominant members of closely related bla OXA-51-like subgroups, which are associated with European clone I (EUI), EUII, and EUIII, respectively [14][15][16]. However, few studies have investigated the correlation between bla OXA-51-like variants with MLST typing.…”

“…Although MLST has many advantages, it is a robust scheme that is often time-consuming, expensive, and labour-intensive [14]. Currently, several studies have reported that sequence-based typing (SBT) of bla OXA-51-like gene variants has potential for application to assess the epidemiological characterization of A. baumannii [14][15][16], but more data are needed.…”