Association Efficiency of Three Ionic Forms of Oxytetracycline to Cationic and Anionic Oil-In-Water Nanoemulsions Analyzed by Diafiltration

The Critical Role of Aromatic-Aromatic Drug-Polymer Interactions to Provide Nanomedicines Containing Chloroquine: A Simple Proposal to Provide High Encapsulation, High Stability, and Prolonged Drug Release

“…The dia ltration method was selected to investigate the kinetic and/or thermodynamic drug entrapment, and the dissociation constants of their equilibrium binding to excipients/formulations [36,37,[46][47][48][49][50][51][52][53].…”

“…A total of 8 samples (approx. 5 mL) were collected and the concentration of CQ in each sample was determined by spectrophotometry [21,53].…”

“…In this paper, dia ltration was performed to determine the fraction of CQ kinetically or thermodynamically bound to PSS. Details of dia ltration procedures, mathematical analysis and results are widely explained in previous studies [36,37,[46][47][48][49][50][51][52][53]. Brie y, the parameters v and u represent the initial fraction of CQ thermodynamically bound to the particles, thus in equilibrium, and the initial fraction of drug bound to the nanoparticles whose release is kinetically controlled, respectively.…”

On the comparison between diafiltration and isothermal titration calorimetry: Determination of the amount of analytes bound to water-soluble polymers

“…A molecular understanding is crucial when it comes to achieve desired properties of a solution system. A number of characterization techniques have been developed in order to study the molecular behavior of systems composed of low molecular-weight species (LMWS) and macromolecules [1][2][3][4][5][6][7][8], since they present important applications in water treatment, delivery systems (of drugs, agrochemicals), sensorial materials, among others [9][10][11][12][13].…”

“…By quantifying the eluted LMWS, a DF profile can be obtained as the DF carries out while keeping constant the volume, pressure and temperature in the DF cell containing both interacting species. The resulting profiles can be used to estimate the apparent dissociation constant, the maximum retention capacities and the initial fraction of LMWS bound to the WSP [11,13,17]. These parameters have been determined for a wide variety of LMWS/WSP systems, allowing the study of long-range electrostatic interactions, short-range aromatic-aromatic interactions, non-chelating and chelating metal/WSP complexes, substrate retention, and oppositely charged polyelectrolytes complexes [7][8][9][10][11][14][15][16][17][18][19][20][21][22].…”