Association mapping and candidate genes for physiological non-destructive traits: Chlorophyll content, canopy temperature, and specific leaf area under normal and saline conditions in wheat

Said, Alaa A.; Moursi, Yasser S.; Sallam, Ahmed

doi:10.3389/fgene.2022.980319

Cited by 10 publications

(8 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of gene annotation revealed three important candidate genes (TraesCS5A02G355900, TraesCS1B02G479100, and TraesCS2D02G509500). These genes are found to be involved in the response to salt stress in wheat with high expression levels under salt stress compared to control based on mining in databases (Said et al 2022).…”

Section: Discussionmentioning

confidence: 99%

“…These signi cant QTLs were located on chromosomes 1B, 2B, 2D, 3A, 3B, 5A, 5B, and 7B. Each exhibited substantial effects, with R2 values ranging from 20.20-30.90% furthermore, gene annotation analysis revealed three signi cant candidate genes (TraesCS5A02G355900, TraesCS1B02G479100 and TraesCS2D02G509500) involved in wheat's response to salt stress, with higher expression levels under salt stress compared to control conditions (Said et al 2022).…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Identification of Candidate Genes in QTL Regions for Biochemical Traits Underlying salt Response in bread wheat (Triticum aestivum) at the Seedling Stage

khakshoor,

azadi,

Frozesh

et al. 2023

Preprint

View full text Add to dashboard Cite

Salinity stress is one of the most important environmental limiting factors in wheat (Triticum aestivum L.) production and developing new salt-tolerant varieties is extremely important. Identification of genes and mechanisms involved in salinity tolerance for molecular modification of this plant is essential In the present study, 186 F12 recombinant inbred lines (RIL) populations were evaluated to identify quantitative trait loci (QTLs) for phenotypic and biochemical characteristics in seedling stage under salt-stress conditions. In total, 21 main-effect QTLs were identified using composite interval mapping (CIM) analysis for antioxidant activity, total protein content and proline, 21 QTL including different traits were detected on 1A1, 4A, 1B, 2B1, 2B2,2B3,3B, 5B1, 6B1, 6B2, 1D and 2D. Candidate genes were identified within QTL regions and gene ontology (GO) enrichment analysis was performed. In total, 10976 candidate genes (CG) were grouped into 99 GO terms (including 59 GO terms involved in the biological process category). Gene prioritization led to the determination of 236 CG, which were involved in hydrogen peroxide catabolic، antioxidant activity، hydrogen peroxide metabolic process، reactive oxygen species metabolic process، metabolic process، catabolic process، monocarboxylic acid metabolic process، regulation of biological quality oxidative stress ، regulation of the biological process, etc. This approach may be proposed to identify new CGs whose corresponding QTL is responsible for complex traits.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Identification of Candidate Genes in QTL Regions for Biochemical Traits Underlying salt Response in bread wheat (Triticum aestivum) at the Seedling Stage

khakshoor,

azadi,

Frozesh

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Improving salt tolerance is a great challenge as it is a very complex trait that is under polygenic control [ 60 ]. To improve wheat production in salinity-affected areas, access to adequate genetic diversity is critical for current and future breeding efforts.…”

Section: Discussionmentioning

confidence: 99%

“…In soybean seedlings were also observed a significant reduction in chlorophyll content at high NaCl level [ 79 ]. The reduction in chlorophyll content caused a reduction in photosynthesis [ 60 ]. The salt-tolerant wheat genotypes revealed higher levels of chlorophyll content compared to the salt sensitive group [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Validation of a QTL on Chromosome 1DS Showing a Major Effect on Salt Tolerance in Winter Wheat

Mohamed

Siddiqui

Oyiga

et al. 2022

IJMS

View full text Add to dashboard Cite

Salt stress is one the most destructive abiotic stressors, causing yield losses in wheat worldwide. A prerequisite for improving salt tolerance is the identification of traits for screening genotypes and uncovering causative genes. Two populations of F3 lines developed from crosses between sensitive and tolerant parents were tested for salt tolerance at the seedling stage. Based on their response, the offspring were classified as salt sensitive and tolerant. Under saline conditions, tolerant genotypes showed lower Na+ and proline content but higher K+, higher chlorophyll content, higher K+/Na+ ratio, higher PSII activity levels, and higher photochemical efficiency, and were selected for further molecular analysis. Five stress responsive QTL identified in a previous study were validated in the populations. A QTL on the short arm of chromosome 1D showed large allelic effects in several salt tolerant related traits. An expression analysis of associated candidate genes showed that TraesCS1D02G052200 and TraesCS5B02G368800 had the highest expression in most tissues. Furthermore, qRT-PCR expression analysis revealed that ZIP-7 had higher differential expressions under saline conditions compared to KefC, AtABC8 and 6-SFT. This study provides information on the genetic and molecular basis of salt tolerance that could be useful in development of salt-tolerant wheat varieties.

show abstract

Genome‐wide scanning to identify and validate single nucleotide polymorphism markers associated with drought tolerance in spring wheat seedlings

Sallam,

Dawood,

Jarquín

et al. 2024

The Plant Genome

Self Cite

View full text Add to dashboard Cite

Unlike other growth stages of wheat, very few studies on drought tolerance have been done at the seedling stage, and this is due to the complexity and sensitivity of this stage to drought stress resulting from climate change. As a result, the drought tolerance of wheat seedlings is poorly understood and very few genes associated with drought tolerance at this stage were identified. To address this challenge, a set of 172 spring wheat genotypes representing 20 different countries was evaluated under drought stress at the seedling stage. Drought stress was applied on all tested genotypes by water withholding for 13 days. Two types of traits, namely morphological and physiological traits were scored on the leaves of all tested genotypes. Genome‐wide association study (GWAS) is one of the effective genetic analysis methods that was used to identify target single nucleotide polymorphism (SNP) markers and candidate genes for later use in marker‐assisted selection. The tested plant materials were genotyped using 25k Infinium iSelect array (25K) (herein after it will be identified as 25K) (for 172 genotypes) and genotyping‐by‐sequencing (GBS) (for 103 genotypes), respectively. The results of genotyping revealed 21,093 25K and 11,362 GBS‐SNPs, which were used to perform GWAS analysis for all scored traits. The results of GWAS revealed that 131 and 55 significant SNPs were controlling morphological and physiological traits, respectively. Moreover, a total of eight and seven SNP markers were found to be associated with more than one morphological and physiological trait under drought stress, respectively. Remarkably, 10 significant SNPs found in this study were previously reported for their association with drought tolerance in wheat. Out of the 10 validated SNP markers, four SNPs were associated with drought at the seedling stage, while the remaining six SNPs were associated with drought stress at the reproductive stage. Moreover, the results of gene enrichment revealed 18 and six pathways as highly significant biological and molecular pathways, respectively. The selection based on drought‐tolerant alleles revealed 15 genotypes with the highest number of different drought‐tolerant alleles. These genotypes can be used as candidate parents in future breeding programs to produce highly drought‐tolerant genotypes with high genetic diversity. Our findings in this study provide novel markers and useful information on the genetic basis of drought tolerance at early growth stages.

show abstract

Association mapping and candidate genes for physiological non-destructive traits: Chlorophyll content, canopy temperature, and specific leaf area under normal and saline conditions in wheat

Cited by 10 publications

References 76 publications

Identification of Candidate Genes in QTL Regions for Biochemical Traits Underlying salt Response in bread wheat (Triticum aestivum) at the Seedling Stage

Identification of Candidate Genes in QTL Regions for Biochemical Traits Underlying salt Response in bread wheat (Triticum aestivum) at the Seedling Stage

Validation of a QTL on Chromosome 1DS Showing a Major Effect on Salt Tolerance in Winter Wheat

Genome‐wide scanning to identify and validate single nucleotide polymorphism markers associated with drought tolerance in spring wheat seedlings

Contact Info

Product

Resources

About