Association Mapping of Verticillium Wilt Disease in a Worldwide Collection of Cotton (Gossypium hirsutum L.)

Abstract

Cotton (Gossypium spp.) is the best plant fiber source in the world and provides the raw material for industry. Verticillium wilt caused by Verticillium dahliae Kleb. is accepted as a major disease of cotton production. The most practical way to deal with verticillium wilt is to develop resistant/tolerant varieties after cultural practices. One of the effective selections in plant breeding is the use of marker-assisted selection (MAS) via quantitative trait loci (QTL). Therefore, in this study, we aimed to discover the genetic markers associated with the disease. Through the association mapping analysis, common single nucleotide polymorphism (SNP) markers were obtained using 4730 SNP alleles. As a result, twenty-three markers were associated with defoliating (PYDV6 isolate) pathotype, twenty-one markers with non-defoliating (Vd11 isolate) pathotype, ten QTL with Disease Severity Index (DSI) of the leaves at the 50–60% boll opening period and eight markers were associated with DSI in the stem section. Some of the markers that show significant associations are located on protein coding genes such as protein Mpv17-like, 21 kDa protein-like, transcription factor MYB113-like, protein dehydration-induced 19 homolog 3-like, F-box protein CPR30-like, extracellular ribonuclease LE-like, putative E3 ubiquitin-protein ligase LIN, pentatricopeptide repeat-containing protein At3g62890-like, fructose-1,6-bisphosphatase, tubby-like F-box protein 8, endoglucanase 16-like, glucose-6-phosphate/phosphate translocator 2, metal tolerance protein 11-like, VAN3-binding protein-like, transformation/transcription domain-associated protein-like, pyruvate kinase isozyme A, ethylene-responsive transcription factor CRF2-like, molybdate transporter 2-like, IRK-interacting protein-like, glycosylphosphatidylinositol anchor attachment 1 protein, U3 small nucleolar RNA-associated protein 4-like, microtubule-associated protein futsch-like, transport and Golgi organization 2 homolog, splicing factor 3B subunit 3-like, mediator of RNA polymerase II transcription subunit 15a-like, putative ankyrin repeat protein, and protein networked 1D-like. It has been reported in previous studies that most of these genes are associated with biotic and abiotic stress factors. As a result, once validated, it would be possible to use the markers obtained in the study in Marker Assisted Selection (MAS) breeding.

Citrus limon L. (lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration

“…The scientifically proven therapeutic activities of C. limon include anti-inflammatory, antimicrobial, antioxidant, anticancer, and antiparasitic actions. Citrus flavonoids may also modulate blood platelet functions and have an anti-aggregatory effect [29,30]. To further explore the pharmacological properties of C. limon, the current investigation focused on the neuro-pharmacological benefits of this plant in the in vivo mouse model.…”

Genetic Diversity, QTL Mapping, and Marker-Assisted Selection Technology in Cotton (Gossypium spp.)

“…Another group of scientists has conducted a GWAS using high-density genotyping with the CottonSNP63K array and identified a total of 15 and 13 QTL for VW and FW resistances were anchored by 30 and 56 significant SNP markers, respectively ( Abdelraheem et al, 2020 ). Similar studies were also conducted by Bardak et al (2021) to discover the genetic markers associated with the Verticillium wilt disease in a Worldwide Collection of Cotton ( Gossypium hirsutum L.; Bardak et al, 2021 ). Through the association mapping analysis, common SNP markers were obtained using 4,730 SNP alleles.…”

Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon

“…Calcium-dependent protein kinases (CDPKs) are a large family of serine/threonine kinases that play an important role in plant defense [ 36 ] and phytohormone signaling pathways [ 37 ], and are responsible for the phosphorylation of a bZIP transcription factor FD, which is required for the formation of the florigen complex [ 38 ]. Glucose-6-phosphate/phosphate translocator 2 gene was found to be associated with verticillium wilt disease [ 39 ] and the C-terminal domain phosphatase-like 1 (CPL1) gene is known to repress stress-inducible gene expression in Arabidopsis [ 40 ] and enhance tolerance to heat stress in chrysanthemum [ 41 ]. Within the QTL ClBFB10.2 region, a total of 18 genes were identified ( Table S3 ).…”