Association Mapping Reveals Genetic Loci Associated with Important Agronomic Traits in Lentinula edodes, Shiitake Mushroom

Abstract

Association mapping is a robust approach for the detection of quantitative trait loci (QTLs). Here, by genotyping 297 genome-wide molecular markers of 89 Lentinula edodes cultivars in China, the genetic diversity, population structure and genetic loci associated with 11 agronomic traits were examined. A total of 873 alleles were detected in the tested strains with a mean of 2.939 alleles per locus, and the Shannon's information index was 0.734. Population structure analysis revealed two robustly differentiated groups among the Chinese L. edodes cultivars (FST = 0.247). Using the mixed linear model, a total of 43 markers were detected to be significantly associated with four traits. The number of markers associated with traits ranged from 9 to 26, and the phenotypic variations explained by each marker varied from 12.07% to 31.32%. Apart from five previously reported markers, the remaining 38 markers were newly reported here. Twenty-one markers were identified as simultaneously linked to two to four traits, and five markers were associated with the same traits in cultivation tests performed in two consecutive years. The 43 traits-associated markers were related to 97 genes, and 24 of them were related to 10 traits-associated markers detected in both years or identified previously, 13 of which had a >2-fold expression change between the mycelium and primordium stages. Our study has provided candidate markers for marker-assisted selection (MAS) and useful clues for understanding the genetic architecture of agronomic traits in the shiitake mushroom.

Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

“…Sixty L. edodes strains from wild and cultivation strains [32,33] were used to screen thermotolerance in this study by primarily observing the mycelial recovery. The well-grown mycelial plugs were inoculated into sawdust plates (78 g of hardwood sawdust, 20 g of wheat bran, 2 g of lime and 20 g of agar in 1 L of distilled water).…”

Comparative Transcriptomics of Flammulina filiformis Suggests a High CO2 Concentration Inhibits Early Pileus Expansion by Decreasing Cell Division Control Pathways

“…For edible mushrooms, pileus expansion leads to the loss of nutrition and the decline of economic value. According to edible mushroom marketing and sales standards, the pileus size and shape are significant agronomic traits and among the most important indexes for product quality [3]. Therefore, it is critical to control pileus expansion during fruiting management of commercially cultivated edible fungus.…”

Analysis of Genetic Diversity and Population Structure of Wild Strains and Cultivars Using Genomic SSR Markers inLentinula edodes

“…Therefore, H E , H O , and PIC values of the cultivars were higher than those of the wild strains. In several recent studies, the analysis of 88 wild strains showed H E of 0.44, H O of 0.29, and PIC of 0.31 using two SSRs and 68 InDels [13], and the analysis of 89 cultivars revealed H E of 0.46, H O of 0.55, and PIC value of 0.38 using 48 SSRs and 249 InDels [36]. Studies conducted in Korea and China have shown that the genetic diversity of L. edodes was also higher in the cultivars than in the wild strains.…”