Association of +405C>G and +936C>T Polymorphisms of the Vascular Endothelial Growth Factor Gene with Sporadic Breast Cancer in North Indians

Abstract

Background: Vascular endothelial growth factor (VEGF), an endothelial cell specific mitogen, has been implicated as a critical factor influencing tumor related angiogenesis. The aim of present study was to evaluate the relationship between VEGF +936C>T and +405C>G polymorphisms of VEGF with risk of breast cancer in Punjab, India. Materials and Methods: We screened DNA samples of 192 sporadic breast cancer patients and 192 unrelated healthy, gender and age matched control individuals for VEGF +936C>T and +405C>G polymorphisms using the PCR-RFLP method. Results: For the VEGF +405C>G polymorphism, we observed significantly increased frequency of GG genotype in cases as compared to controls and strong association of +405GG genotype was observed with three fold risk for breast cancer (OR=3.07; 95%CI 1.41-6.65; p=0.003). For the +936C>T polymorphism, significant associations of CT and combined CT+TT genotypes were observed with elevated risk of breast cancer (p=0.021; 0.023). The combined genotype combinations of GG-CC and GG-CT of +405C>G and +936C>T polymorphisms were found to be significantly associated with increased risk of breast cancer (p=0.04; 0.0064). Conclusions: The findings of the present study indicated significant associations of VEGF +936C>T and +405C>G polymorphisms with increased breast cancer risk in patients from Punjab, North India.

In silico pathway analysis based on Chromosomal Instability in Breast Cancer Patients

“…NCOA1 promotes angiogenesis in breast tumors by enhancing the transcription of VEGFa via HIFα and AP-1 [95]. Previous studies from our lab on breast cancer patients from same region have reported association of VEGF polymorphisms +405C>G, +936C>T, -2549 Insertion/Deletion, −152G/A, −116G/A, −165C/T and −141A/C with breast cancer risk but no association of VEGF -417C/T, -172C/A and -160C/T and HIF1α polymorphisms (g.C111A, g.C1772T and g.G1790A) with breast cancer risk [96][97][98][99].…”

Predictive value of vascular endothelial growth factor polymorphisms on the risk of renal cell carcinomas

“…VEGF expression can play a role in regulating angiogenesis is involved in promoting endothelial cell proliferation and regulating the extracellular matrix in the blood vessels (Roy et al, 2006;Kushner and Bautch, 2013). Several functional SNPs in the VEGF gene can alter the expression levels of VEGF protein in cancer cells, thus influencing the tumor angiogenic activity and accelerating carcinogenesis such as bladder cancer, breast cancer, lung cancer, colorectal cancer, prostate cancer, ovarian cancer, and osteosarcoma (Li et al, 2010;Jaiswal et al, 2013;Kapahi et al, 2014;Deng et al, 2014;Lau et al, 2014;Chen et al, 2014;Wang et al, 2014). Jaiswal et al (2013) reported that VEGF polymorphisms may play a significant role in mediating bladder cancer.…”

“…Jaiswal et al (2013) reported that VEGF polymorphisms may play a significant role in mediating bladder cancer. Kapahi et al (2014) examined an Indian population and found that the VEGF +936C>T and +405C>G polymorphisms were associated with an increased risk of breast cancer. Lau et al (2014) conducted a study in a Malaysian population and found that the VEGF gene polymorphism has a role in the development of colorectal cancer.…”

“…Several previous studies reported that polymorphisms in VEGF play an important role in the development of several kinds of cancers such as bladder cancer, breast cancer, lung cancer, and renal cell carcinoma (Ajaz et al, 2011;Maeda et al, 2013;Kapahi et al, 2014;Yang et al, 2014). However, few studies have examined the association between VEGF polymorphisms and RCC risk, and the results are inconsistent (Zhang et al, 2013;Zhong et al, 2014).…”

Effect of polymorphisms of vascular endothelial growth factor on prognosis in osteosarcoma patients

“…VEGF is a potent regulator of angiogenesis and thus plays a role in the development and prognosis of solid tumors (Kapahi et al, 2014;James et al, 2014). Several common single-nucleotide polymorphisms (SNPs) in the VEGF gene have been reported to have a role in gene expression.…”