Association of ADIPOQ +45T>G polymorphism with body fat mass and blood levels of soluble adiponectin and inflammation markers in a Mexican-Mestizo population

Abstract

PurposeObesity is a disease with genetic susceptibility characterized by an increase in storage and irregular distribution of body fat. In obese patients, the decrease in the Adiponectin gene (ADIPOQ) expression has been associated with a systemic low-grade inflammatory state. Our aim was to investigate the relationship between ADIPOQ +45T>G gene simple nucleotide polymorphism (SNP rs2241766) with serum adiponectin (sAdiponectin), distribution of body fat storage, and inflammation markers.Subjects and methodsIn this cross-sectional study, 242 individuals from Western Mexico characterized as Mexican-Mestizo and classified by body mass index (BMI), were included. Anthropometrics, body composition, body fat distribution, and inflammation markers were measured by routine methods. Genotypes were characterized using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and sAdiponectin by the ELISA method. A P-value <0.05 was considered the statistically significant threshold.ResultssAdiponectin is associated with BMI (P < 0.001) and the genotypes (P < 0.001 to 0.0046) GG (8169 ± 1162 ng/mL), TG (5189 ± 501 ng/mL), and TT (3741 ± 323 ng/mL), but the SNP ADIPOQ +45T>G is not associated with BMI. However, the detailed analysis showed association of this SNP with a pattern of fat distribution and correlations (P < 0.05) with inflammation markers and distribution of body fat storage (Pearson’s r = −0.169 to −0.465) were found.ConclusionIn this study, we have suggested that the ADIPOQ +45G allele could be associated with distribution of body fat storage in obesity. On the other hand, as no association was observed between ADIPOQ +45T>G gene polymorphism and obesity, it cannot be concluded that the ADIPOQ +45G allele is responsible for the increase of adiponectin levels.

The association of circulating adiponectin and + 45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population

“…It has been reported that SNP +45 T > G in the adiponectin gene could affect the expression of adiponectin by influencing adiponectin mRNA processing or stability [ 21 ]. It has also been reported that SNP +45 T > G in the adiponectin gene has an association with circulating levels of adiponectin [ 28 , 30 ]. However, our results in accordance with data from other studies [ 31 ] showed that there are no association between circulating level of adiponectin, and SNP +45 T > G genotypes.…”

Association of Adiponectin Gene (ADIPOQ) rs2241766 Polymorphism with Obesity in Adults: A Meta-Analysis

“…A variant of ADIPOQ -rs2241766 G/T was reported to be associated with adiponectin level [14], [35]. Research showed that this synonymous mutation may affect steady-state mRNA levels by altering RNA splicing or stability [42], suggesting an allele-specific differential expression of adiponectin.…”

Five gene variants in nonagenarians, centenarians and average individuals

“…Guzman-Ornelas et al . [ 23 ] evaluated the same gene polymorphism (rs2241766) as the one we studied, and did not observe any relationship between this polymorphism and adiponectin levels in Mexican-Mestizo individuals. In our study, we also found no difference in ADIPOQ ; rs2241766 gene polymorphism frequency between the 2 studied groups.…”