Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Liu, Ping; Soupir, Michelle L.; Zwonitzer, Martha R.; Huss, Bridgette; Jarboe, Laura R.

doi:10.1128/aem.00717-11

Cited by 19 publications

(29 citation statements)

References 43 publications

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“…For example, with a simple estimation using the Stoke's Law, E. coli attached to 63–125 μ m quartz particles settled in 5 min (Liu et al . ). Filtration allows quantitative and hierarchical separation as particles of different sizes are retained via sieving the suspension through membranes of a known pore size (USEPA ).…”

Section: Attachmentmentioning

confidence: 97%

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Cellular, particle and environmental parameters influencing attachment in surface waters: a review

et al. 2015

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SummaryEffective modelling of the fate and transport of water-borne pathogens is needed to support federally required pollution-reduction plans, for water quality improvement planning, and to protect public health. Lack of understanding of microbial-particle interactions in water bodies has sometimes led to the assumption that bacteria move in surface waters not associated with suspended mineral and organic particles, despite a growing body of evidence suggesting otherwise. Limited information exists regarding the factors driving interactions between micro-organisms and particles in surface waters. This review discusses cellular, particle and environmental factors potentially influencing interactions and in-stream transport. Bacterial attachment in the aquatic environment can be influenced by properties of the cell such as genetic predisposition and physiological state, surface structures such as flagella and fimbriae, the hydrophobicity and electrostatic charge of the cell surface, and the presence of outer-membrane proteins and extracellular polymeric substances. The mechanisms and degree of attachment are also affected by characteristics of mineral and organic particles including the size, surface area, charge and hydrophobicity. Environmental conditions such as the solution chemistry and temperature are also known to play an important role. Just as the size and surface of chemical particles can be highly variable, bacterial attachment mechanisms are also diverse.

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Section: Attachmentmentioning

confidence: 97%

“…; Liu et al . ). A lack of understanding of the mechanisms of bacterial attachment to particles in eroded soil, stream‐bottom sediments and suspended sediments leads to inaccuracy in modelling bacterial transport at the watershed scale – and therefore to inaccurate TMDLs.…”

Section: Introductionmentioning

confidence: 97%

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

et al. 2015

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“…This process was guided by the use of proteomic analysis and development of a competitive attachment assay, modified from previous work (Liu et al, 2011). This analysis includes consideration of flagella and OMPs, such as Outer Membrane Protein A (OmpA).…”

Section: Introductionmentioning

confidence: 99%

Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

et al. 2017

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Understanding the genetic factors that govern microbe-sediment interactions in aquatic environments is important for water quality management and reduction of waterborne disease outbreaks. Although chemical properties of bacteria have been identified that contribute to initiation of attachment, the outer membrane proteins that contribute to these chemical properties still remain unclear. In this study we explored the attachment of 78 Escherichia coli environmental isolates to corn stover, a representative agricultural residue. Outer membrane proteome analysis led to the observation of amino acid variations, some of which had not been previously described, in outer membrane protein A (OmpA) at 10 distinct locations, including each of the four extracellular loops, three of the eight transmembrane segments, the proline-rich linker and the dimerization domain. Some of the polymorphisms within loops 1, 2, and 3 were found to significantly co-occur. Grouping of sequences according to the outer loop polymorphisms revealed five distinct patterns that each occur in at least 5% of our isolates. The two most common patterns, I and II, are encoded by 33.3 and 20.5% of these isolates and differ at each of the four loops. Statistically significant differences in attachment to corn stover were observed among isolates expressing different versions of OmpA and when different versions of OmpA were expressed in the same genetic background. Most notable was the increased corn stover attachment associated with a loop 3 sequence of SNFDGKN relative to the standard SNVYGKN sequence. These results provide further insight into the allelic variation of OmpA and implicate OmpA in contributing to attachment to corn stover.

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“…A study by Liu et al . () used Stoke's equation to determine the settling time for quartz to separate freely suspended E. coli from quartz‐attached E. coli (Liu et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…In the aquatic environment, particles with larger density should settle out more quickly than particles with smaller densities. A study by Liu et al (2011) used Stoke's equation to determine the settling time for quartz to separate freely suspended E. coli from quartz-attached E. coli (Liu et al 2011). Filtration is characterized by its ability to remove particles via a sieving mechanism based on the size of the membrane pores relative to that of the particulate matter (USEPA 2005).…”

Section: Introductionmentioning

confidence: 99%

Flow cytometry is a promising and rapid method for differentiating between freely suspended Escherichia coli and E. coli attached to clay particles

et al. 2014

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Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Cited by 19 publications

References 43 publications

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

Flow cytometry is a promising and rapid method for differentiating between freely suspended Escherichia coli and E. coli attached to clay particles

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