Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit

Gurung, Jeetendra; Khyriem, Annie Bakorlin; Banik, Amit; Lyngdoh, Wihiwot Valarie; Choudhury, Basabdatta; Bhattacharyya, Prithwis

doi:10.4103/0972-5229.118416

Cited by 86 publications

(32 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, the current analysis provides the first statistical data linking bacterial production of biofilms with the persistence of traumatic wound infections and corroborates earlier reports on the potential virulence of the bacterial biofilm phenotype [ 25 ]. Biofilm formation, in our analysis, was largely attributed to multidrug-resistant A. baumannii , which corresponds to a prior study reporting a statistical increase in the proportion of A. baumannii isolates that were biofilm-producing and multidrug-resistant (73%) when compared to P. aeruginosa (57%) [ 41 ]. In addition, a recent analysis utilized clinical isolates collected from patients including military personnel admitted to a U.S. MTF to evaluate biofilm formation.…”

Section: Discussionsupporting

confidence: 83%

Biofilms and persistent wound infections in United States military trauma patients: a case–control analysis

et al. 2014

View full text Add to dashboard Cite

BackgroundComplex traumatic injuries sustained by military personnel, particularly when involving extremities, often result in infectious complications and substantial morbidity. One factor that may further impair patient recovery is the persistence of infections. Surface-attached microbial communities, known as biofilms, may play a role in hindering the management of infections; however, clinical data associating biofilm formation with persistent or chronic infections are lacking. Therefore, we evaluated the production of bacterial biofilms as a potential risk factor for persistent infections among wounded military personnel.MethodsBacterial isolates and clinical data from military personnel with deployment-related injuries were collected through the Trauma Infectious Disease Outcomes Study. The study population consisted of patients with diagnosed skin and soft-tissue infections. Cases (wounds with bacterial isolates of the same organism collected 14 days apart) were compared to controls (wounds with non-recurrent bacterial isolates), which were matched by organism and infectious disease syndrome. Potential risk factors for persistent infections, including biofilm formation, were examined in a univariate analysis. Data are expressed as odds ratios (OR; 95% confidence interval [CI]).ResultsOn a per infected wound basis, 35 cases (representing 25 patients) and 69 controls (representing 60 patients) were identified. Eight patients with multiple wounds were utilized as both cases and controls. Overall, 235 bacterial isolates were tested for biofilm formation in the case–control analysis. Biofilm formation was significantly associated with infection persistence (OR: 29.49; CI: 6.24-infinity) in a univariate analysis. Multidrug resistance (OR: 5.62; CI: 1.02-56.92), packed red blood cell transfusion requirements within the first 24 hours (OR: 1.02; CI: 1.01-1.04), operating room visits prior to and on the date of infection diagnosis (OR: 2.05; CI: 1.09-4.28), anatomical location of infected wound (OR: 5.47; CI: 1.65-23.39), and occurrence of polymicrobial infections (OR: 69.71; CI: 15.39-infinity) were also significant risk factors for persistent infections.ConclusionsWe found that biofilm production by clinical strains is significantly associated with the persistence of wound infections. However, the statistical power of the analysis was limited due to the small sample size, precluding a multivariate analysis. Further data are needed to confirm biofilm formation as a risk factor for persistent wound infections.

show abstract

Section: Discussionsupporting

confidence: 83%

Biofilms and persistent wound infections in United States military trauma patients: a case–control analysis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Consistent with our findings, their results provided evidence that the ability to develop biofilm may be a crucial determinant in the survival of susceptible or non-MDR bacteria. However, another study indicated a significant association between high biofilm-forming ability and high level of antibiotic resistance (7). Investigation of the relationship between biofilm-forming ability and antibiotic resistance phenotypes among A. baumannii major IC types (ICI and ICII) also exhibited such a significant relationship.…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

Rahimi

Farshadzadeh

Taheri

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Understanding of the biological factors responsible for prevalence and persistence of Acinetobacter baumannii in hospital settings is critical to prevent and control the corresponding nosocomial infections. Objectives: This study was conducted to investigate whether the biofilm-forming ability is associated with the emergence of different antibiotic resistance phenotypes [multidrug resistance (MDR)/extensively drug resistance (XDR) and non-MDR] of A. baumannii. Methods: The capacities of biofilm formation in 80 clinical A. baumannii strains isolated from hospitalized burn patients in Bushehr, Iran, were assessed using the crystal violet staining. Results: The statistical analysis of the relationship between biofilm-forming ability and antibiotic resistance phenotypes among all clinical A. baumannii strains using one-way ANOVA test indicated that biofilm formation capacity of non-MDR A. baumannii isolates was significantly higher than that of MDR and XDR ones (P < 0.001), suggesting an inverse relationship between biofilm formation capacity and the acquisition of MDR/XDR phenotypes. Major international clonal types (ICI and ICII) also exhibited such a significant relationship (P < 0.0001). However, the investigation of A. baumannii IC variants showed no significant relationship between these phenotypes. Conclusions: Given that non-MDR A. baumannii isolates in major IC types were observed to form a strong biofilm compared to MDR/XDR ones, it seems that biofilm may play a key role in the persistence and survival of A. baumannii isolates with an inadequate level of antibiotic resistance. Furthermore, the results showed that the relationship between biofilm and antibiotic resistance phenotypes might be affected by the IC types (major IC types or IC variants).

show abstract

“…Bacterial study. Five collection strains and widely known biofilm producers, that is, S. aureus 15981 (77-80), S. epidermidis ATCC 35984 (81)(82)(83), E. coli ATCC 25922 (84)(85)(86), P. aeruginosa ATCC 27853 (87)(88)(89), and S. maltophilia ATCC 13637 (90,91), were used. All the strains were kept frozen at Ϫ80°C until the experiments were performed.…”

Section: Methodsmentioning

confidence: 99%

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Aguilera-Correa

Mediero

Conesa-Buendía

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

Joint prosthesis failure is mainly related to aseptic loosening and prosthetic joint infections, both of which are associated with high morbidity and substantial costs for patients and health systems. The development of a biomaterial that is capable of stimulating bone growth while minimizing bacterial adhesion would reduce the incidence of prosthetic failure. We report antibacterial and osteostimulatory effects in a novel fluorine-phosphorus (F-P)-doped TiO2 oxide film grown on Ti-6Al-4V alloy with a nanostructure of bottle-shaped nanotubes (bNT) using five bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia) and MCT3T3-E1 osteoblastic cells. The interaction between the bacteria and bNT Ti-6Al-4V was complex, as the adhesion of four bacterial species decreased (two staphylococcus species, E. coli, and S. maltophilia), and the viability of staphylococci and S. maltophilia also decreased because of the aluminum (Al) released by bNT Ti-6Al-4V. This released Al can be recruited by the bacteria through siderophores and was retained only by the Gram-negative bacteria tested. P. aeruginosa showed higher adhesion on bNT Ti-6Al-4V than on chemically polished (CP) samples of Ti-6Al-4V alloy and an ability to mobilize Al from bNT Ti-6Al-4V. The cell adhesion and proliferation of MCT3T3-E1 osteoblastic cells significantly increased at 48 and 168 h, as did the matrix mineralization of these cells and the gene expression levels of three of the most important markers related to bone differentiation. According to our results, the bNT Ti-6Al-4V alloy could have clinical application, preventing infection and stimulating bone growth and thus preventing the two main causes of joint prosthesis failure. IMPORTANCE This work evaluates F-P-doped bNT Ti-6Al-4V from microbiological and cellular approaches. The bacterial results highlight that the antibacterial ability of bNT Ti-6Al-4V is the result of a combination of antiadhesive and bactericidal effects exerted by Al released from the alloy. The cell results highlight that F-P bNT Ti-6Al-4V alloy increases osseointegration due to modification of the chemical composition of the alloy resulting from P incorporation and not due to the nanostructure, as reported previously. A key finding was the detection of Al release from inside the bNT Ti-6Al-4V nanostructures, a result of the nanostructure growth during the anodizing process that is in part responsible for its bactericidal effect.

show abstract

Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit

Cited by 86 publications

References 15 publications

Biofilms and persistent wound infections in United States military trauma patients: a case–control analysis

Biofilms and persistent wound infections in United States military trauma patients: a case–control analysis

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Contact Info

Product

Resources

About