Association of Blastocystis hominis with signs and symptoms of human disease

SummaryIsolates of Blastocystis hominis from infected immigrant workers from Indonesia, Bangladesh and infected individuals from Singapore and Malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. Viability of the cells was assessed using eosin-brilliant cresyl blue which stained viable cells green and nonviable cells red. The Bangladeshi and Singaporean isolates were nonviable even at the lowest concentration of metronidazole at 0.01 mg/ml. The parasites of the Malaysian isolate were resistant to a concentration of 0.01 mg/ml, whereas 40% of the initial inoculum of parasites from the Indonesian isolate at day one were still viable in cultures with 1.0 mg/ml metronidazole. The study shows that isolates of B. hominis of different geographical origin have different levels of resistance to metronidazole. The search for more effective drugs to eliminate the parasite appears inevitable, especially since surviving parasites from metronidazole cultures show greater ability to multiply in subcultures than controls.

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“…B. hominis has been associated with a variety of diseases ( Gallagher & Venglarcik 1985; Sheehan et al . 1986 ; Tsang et al .…”

Section: Discussionmentioning

confidence: 99%

“…Blastocystis hominis , a protozoan parasite commonly found in the human gut, especially in the tropics, is known to cause diarrhoea ( Sheehan et al . 1986 ; Miller & Minshew 1988).…”

Section: Introductionmentioning

confidence: 99%

Isolate resistance of Blastocystis hominis to metronidazole

Haresh

Suresh

Anuar

et al. 1999

Tropical Med Int Health

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“…The medical records allowed us to look for an association between defined subgroups and the disease symptoms attributed to B. hominis (Sheehan et al 1986;Zierdt 1991) which included diarrhoea, abdominal Silberman et al (1996). Asterisks indicate 100% identity, a minus possible identity, N stands for all possible bases.…”

Section: Association Of Subgroups With Medical Recordsmentioning

confidence: 99%

“…To date it is not known whether this organism is a commensal or a parasite of man. The conflicting reports on this topic (Sheehan et al 1986;Miller & Minshew 1988;Anonymous 1991;Keystone 1995;Markell 1995;Shlim et al 1995) might have their roots in the existence of different B. hominis subspecies with the possession of a different pathogenic potential, as has been shown for the morphologically indistinguishable Entamoeba histolytica and Entamoeba dispar (Diamond & Clark 1993) or for the plant-pathogenic mycoplasma-like organisms (Schneider et al 1993). Differences among B. hominis isolates revealed by immunological data and genetic analysis (Upcroft et al 1989;Kukoschke & Müller 1991;Boreham et al 1992;Müller 1994;Yoskikawa et al 1996) speak for a subgrouping of this organism.…”

Section: Introductionmentioning

confidence: 99%

Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR‐amplified 16S‐like rDNA

Böhm-Gloning

Knobloch

Walderich

1997

Tropical Med Int Health

124

103

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SummaryDNA polymorphism of Blastocystis hominis isolates was examined by the amplification of a gene fragment coding for the 16S-like rRNA. Using identical primers, fragments of approximately 850 bp were amplified from 110 B. hominis isolates and fragments of 1.1kbp were amplified from 48 isolates. Digestion of the amplification products with the restriction enzymes HinfI, RsaI, and AluI revealed different profiles for each fragment length. Subgroup I and II, resulting from digestion of the smaller 850 bp fragment, have identical HinfI and AluI restriction bands, subgroup III and IV have identical RsaI fragments after digestion of the 1.1kbp DNA. Subgroup V resembles subgroup III in a few bands after the RsaI and AluI restriction, respectively. Ninety (54%) of the isolates studied were assigned to subgroup I, 20 (12%) to subgroup II, 35 (21%) to subgroup III, 12 (7%) to subgroup IV, and 1 (1%) to subgroup V. Five (3%) of the examined people were coinfected with B. hominis of subgroup I and III, 3 (2%) carried B. hominis of subgroup I and II. These results show that there are 5 B. hominis subgroups none of which was found to be significantly correlated with the reported disease.keywords Blastocystis hominis, 16S-like rDNA, PCR, restriction analysis correspondence Dr. Brigitte Böhm-Gloning,

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“…Blastocystis hominis is frequently found in human faeces as vacuolar cells or cysts, and is believed to be spread via the faecal-oral route (Boreham & Stenzel 1993;Singh et al 1995;Stenzel & Boreham 1991;Zierdt 1991). The prevalence of B. hominis in man is between 1.6 and 16% in industrialized countries (Sheehan et al 1986;Sun et al 1989) and more than 50% in developing countries (Ashford & Atkinson 1992;Torres et al 1992). The pathogenic potential of B. hominis is still a matter of discussion (Doyle et al 1990;Garavelli et al 1991;Kain et al 1987;Markell & Udkow 1986;Miller & Minshew 1988;Senay & MacPherson 1990;Sun et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Isoenzyme patterns of Blastocystis hominis patient isolates derived from symptomatic and healthy carriers

Gericke

Burchard

Knobloch

et al. 1997

Tropical Med Int Health

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SummaryIsolates of Blastocystis hominis derived from patients with intestinal symptoms and from healthy carriers were cultured in vitro and isoenzyme patterns of hexokinase (E.C. 2.7.1.1), phosphoglucomutase (E.C. 2.7.5.1) and glucose phosphate isomerase (E.C. 5.3.1.9) were investigated to find evidence for pathogenic and non-pathogenic subspecies of B. hominis. For this purpose we examined 2000 patients of the out-patient department of the Institute for Tropical Medicine in Tübingen. From these, we obtained 232 B. hominis patient isolates; 119 isolates could be tested for the three isoenzymes. Blastocystis hominis possesses 5 patterns for hexokinase, 11 for phosphoglucomutase and 35 for glucose phosphate isomerase, showing that B. hominis is highly polymorphic. However, there was no correlation between isoenzyme patterns and disease of patients.keywords Blastocystis, patient isolates, isoenzyme patterns correspondence Dr rer. nat. Brigitte Walderich,

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Association of Blastocystis hominis with signs and symptoms of human disease

Cited by 137 publications

References 10 publications

Isolate resistance of Blastocystis hominis to metronidazole

Isolate resistance of Blastocystis hominis to metronidazole

Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR‐amplified 16S‐like rDNA

Isoenzyme patterns of Blastocystis hominis patient isolates derived from symptomatic and healthy carriers

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