Background: Burnout is a growing problem among medical staff worldwide and empathy has been described as an essential competence to attenuate burnout. Previous studies found job satisfaction and job commitment were affected by the empathy and associated with burnout. This study explores the effect and mechanism of empathy on burnout on medical staff and investigates the mediating role of job satisfaction and job commitment in the relationship between empathy and burnout among medical staff.Methods: Based on a self-administered questionnaire, 335 responses from medical staff in Tianjin City, China, yielded data on socio-demographic characteristics, empathy, burnout, job satisfaction and job commitment. Bivariate correlation and structured equation modeling (SEM) analyzed the relationships between empathy, job satisfaction, job commitment and burnout multi-group invariant analysis was used to evaluate whether the model was consistent across different type and level of hospitals and different job and employment type subgroups. Results: A total of 202 (60.3%) medical staff had low level burnout, 115 (34.3%) staff had the moderate level and 18 (5.4%) staff had the high level burnout. The results of the SEM showed that empathy not only have a direct negative effect on burnout (\(\beta =-0.401, Pcript>\), but also had an indirect impact through job satisfaction (\(\beta =-0.373, Pcript>\) and job commitment (\(\beta =0.489, Pcript>\). Job commitment was negatively associated burnout (\(\beta =-0.513, Pcript>\) but, unexpectantly, job satisfaction was positively associated with burnout (\(\beta =0.177, Pcript>\). The results also indicated the model was consistent across employment type (\(\varDelta {\chi }^{2}\) = 5.904, p > 0.05) and hospital type (\(\varDelta {\chi }^{2}\) = 7.748, p > 0.05), but was inconsistent across hospital level (\(\varDelta {\chi }^{2}\) = 42.930, p < 0.05) and job type (\(\varDelta {\chi }^{2}\) = 52.912, p < 0.05).Conclusions: Our results confirm the important role that empathy plays in the prevention of burnout and by managing job satisfaction and increasing the job commitment burnout can be attenuated. We recommend that government should increase pay for medical staff and continue to reform the resourcing of different hospital levels and that hospital managers implement additional training and strengthening psychological testing and counseling, to reduce medical staff burnout.