Association of calcium hypochlorite, reciprocating instrumentation and photodynamic therapy: Antimicrobial analysis and effects on root dentin structure

Souza, Matheus Albino; Lago, Bruna Luiza Tozatti; Pletsch, Amália; Binotto, Amanda; Poletti, Andressa; Rodrigues, Fernando Tolfo; Ricci, Rafaela; Bischoff, Karolina Frick; Dias, Caroline Tumelero; Palhano, Huriel Scartazzini; Lago, Carlo Theodoro Raimundy; Farina, Ana Paula; Cecchin, Doglas; Bervian, Juliane; Figueiredo, José Antônio Poli de

doi:10.1016/j.pdpdt.2019.101625

Cited by 8 publications

(2 citation statements)

References 27 publications

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“…The rotary and reciprocating instrumentation provided advances in the shaping stage of root canal treatment, making this instrumentation faster. However, the contact time of chemical auxiliary substances with root canals was also reduced, decreasing the antimicrobial properties of chemical substances [29,30]. In the present study, the instrumentation was not performed during the decontamination process, because present study aimed to evaluate the intrinsic antimicrobial potential of the tested substances.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial action, cytotoxicity, cleaning ability and erosive potential of Dentaqua solution compared with sodium hypochlorite

Souza,

Steier,

Vanin

et al. 2024

Preprint

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Objectives: This study aimed to compare the antimicrobial action, cytotoxicity, cleaning ability, and erosion of dentine of hypochlorous acid (HClO) obtained from an electrolytic device at two different concentrations (Dentaqua) and three concentrations of sodium hypochlorite (NaOCl). Methods: Microbiological test - The root canals of sixty single-rooted extracted human teeth were inoculated with Enterococcus faecalis and divided into 6 groups (n=10), according to decontamination protocol: DW (control); 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl; 250 ppm HClO and 500 ppm HClO. The counting of colony-forming units evaluated the decontamination potential of each group, calculating the bacterial percentage reduction. Cytotoxicity test - Cytotoxicity was evaluated after inoculation of the same tested protocols in fibroblastic cells for 3 min, calculating the cell viability percentages. Specifical statistical analysis was performed (α = 5%). Cleaning ability and erosion - Fifty-six single-rooted bovine lower incisors were divided into seven groups of 8 roots each, being the test groups 1% NaOCl; 2.5% NaOCl; 5,25% NaOCl; 250 ppm HClO and 500 ppm HClO, and a negative and positive control. Negative control was not contaminated, and the other groups were inoculated with Enterococcus faecalis. SEM images were ranked as from the cleanest to the least clean. Erosion was also assessed, being ranked from the least to the most eroded dentine. Results: The highest bacterial reduction was observed in experimental groups, with no statistical differences between them (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences between them (p < 0.05). 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl and 500 ppm HClO displayed the cleanest areas. All sodium hypochlorite groups displayed erosion with higher ranks with greater concentration, while hypochlorous acid did not display any erosion regardless the concentration. Conclusions: It is possible to conclude that HClO obtained from an electrolytic device presented high antimicrobial activity and low cytotoxicity in both tested concentrations. 500 ppm HClO did not display erosion and showed great cleaning ability. Clinical relevance: The use of 500 ppm hypochlorous acid may reduce unfavorable behavior of sodium hypochlorite whilst maintaining its antimicrobial action.

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Section: Discussionmentioning

confidence: 97%

Antimicrobial action, cytotoxicity, cleaning ability and erosive potential of Dentaqua solution compared with sodium hypochlorite

Souza,

Steier,

Vanin

et al. 2024

Preprint

Self Cite

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“…The clinical symptoms and severity of post-endodontic pain is in direct proportion to the concentration and amount of sodium hypochlorite excreted from the root apex. Standardization of irrigation protocols, such as control of needle penetration depth, can eliminate the effect of intraoperative variables on pain scores [1,29].…”

Section: Apical Extrusion Of Intracanal Content Postendodontic Painmentioning

confidence: 99%

Analysis of a glide path creation necessity at the initial stages of endodontic treatment

Khabadze

Generalova

2021

Èndodontiâ today

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Aim. Article is to detail the positive and negative aspects associated with the creation of a glide path with various types of endodontic instruments.Materials and methods. During a systematic review of the literature, Russian – and English-language publications were studied in the electronic databases PubMed, Google Scholar. Articles, the content of which concerns the methods of creating a glide path, as well as possible complications were included in analysis.Results. 60 articles were reviewed during the review. After analyzing the literature according to the inclusion and exclusion criteria, the total number of publications has become 32.Conclusions. According to the studied literature, it can be said that the glide path provides a predictable behavior of rotary tools and it is a recommended condition for achieving the success of endodontic treatment at the stage of canal mechanical treatment and preventing iatrogenic complications.

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Antimicrobial action, cytotoxicity and erosive potential of hypochlorous acid obtained from an electrolytic device compared with sodium hypochlorite

Souza,

Steier,

Vanin

et al. 2024

Clin Oral Invest

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Objectives: This study aimed to compare the antimicrobial action, cytotoxicity, cleaning ability, and erosion of dentine of hypochlorous acid (HClO) obtained from an electrolytic device at two different concentrations (Dentaqua) and three concentrations of sodium hypochlorite (NaOCl).Methods: Microbiological test -The root canals of sixty single-rooted extracted human teeth were inoculated with Enterococcus faecalis and divided into 6 groups (n=10), according to decontamination protocol: DW (control); 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl; 250 ppm HClO and 500 ppm HClO. The counting of colony-forming units evaluated the decontamination potential of each group, calculating the bacterial percentage reduction. Cytotoxicity test -Cytotoxicity was evaluated after inoculation of the same tested protocols in broblastic cells for 3 min, calculating the cell viability percentages. Speci cal statistical analysis was performed (α = 5%). Cleaning ability and erosion -Fifty-six single-rooted bovine lower incisors were divided into seven groups of 8 roots each, being the test groups 1% NaOCl; 2.5% NaOCl; 5,25% NaOCl; 250 ppm HClO and 500 ppm HClO, and a negative and positive control. Negative control was not contaminated, and the other groups were inoculated with Enterococcus faecalis. SEM images were ranked as from the cleanest to the least clean. Erosion was also assessed, being ranked from the least to the most eroded dentine.Results: The highest bacterial reduction was observed in experimental groups, with no statistical differences between them (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences between them (p < 0.05). 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl and 500 ppm HClO displayed the cleanest areas. All sodium hypochlorite groups displayed erosion with higher ranks with greater concentration, while hypochlorous acid did not display any erosion regardless the concentration.Conclusions: It is possible to conclude that HClO obtained from an electrolytic device presented high antimicrobial activity and low cytotoxicity in both tested concentrations. 500 ppm HClO did not display erosion and showed great cleaning ability.Clinical relevance: The use of 500 ppm hypochlorous acid may reduce unfavorable behavior of sodium hypochlorite whilst maintaining its antimicrobial action.

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Association of calcium hypochlorite, reciprocating instrumentation and photodynamic therapy: Antimicrobial analysis and effects on root dentin structure

Cited by 8 publications

References 27 publications

Antimicrobial action, cytotoxicity, cleaning ability and erosive potential of Dentaqua solution compared with sodium hypochlorite

Antimicrobial action, cytotoxicity, cleaning ability and erosive potential of Dentaqua solution compared with sodium hypochlorite

Analysis of a glide path creation necessity at the initial stages of endodontic treatment

Antimicrobial action, cytotoxicity and erosive potential of hypochlorous acid obtained from an electrolytic device compared with sodium hypochlorite

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