Association of cell surface heparan sulfate proteoglycans of Schwann cells with extracellular matrix proteins

Carey, David J.; Crumbling, D M; Stahl, Richard C.; Evans, D M

doi:10.1016/s0021-9258(17)30549-5

Cited by 34 publications

(1 citation statement)

References 38 publications

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“…Decellularised SIS contained heparan sulphate proteoglycans (HSPGs) that may have multiple functions in tissue repairing, such as attracting growth factors, providing cell attachment sites, and sequestering matrix-degrading enzymes [18]. The endogenous HSPGs in nerve tissue were known to promote axon growth and nerve repairing [19][20][21], but their functions in transplanted biomaterials have not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

et al. 2023

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Peripheral nerve regeneration (PNR) following trauma requires the reconstruction of the extracellular matrix (ECM) and the proper stimulation of growth factors. Decellularized small intestine submucosa (SIS) has been extensively used as an ECM scaffold for tissue repair, but its potential to enhance the effects of exogenous growth factors on PNR is not well understood. In this study, we evaluated the effects of SIS implantation combined with glial cell-derived growth factor (GDNF) treatment on PNR in a rat neurorrhaphy model. We found that both SIS and regenerating nerve tissue expressed syndecan-3 (SDC3), one of major heparan sulphate proteoglycans (HSPG) in nerve tissue, and that SDC3 interacted with GDNF in the regenerating nerve tissue. Importantly, the SIS-GDNF combined treatment enhanced the recovery of neuromuscular function and β3-tubulin-positive axonal outgrowth, indicating an increase in the number of functioning motor axons connecting to the muscle after neurorrhaphy. Our findings suggest that the SIS membrane offers a new microenvironment for neural tissue and promotes neural regeneration based on SDC3-GDNF signaling, providing a potential therapeutic approach for PNR.

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Section: Introductionmentioning

confidence: 99%

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

et al. 2023

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General Strategies for the Characterization of Carbohydrates from Recombinant Glycoprotein Therapeutics

Gerwig¹,

Damm²

1998

Bioseparation and Bioprocessing

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Sulfatase‐mediated manipulation of the astrocyte‐Schwann cell interface

O’Neill

Lindsay

Pantiru

et al. 2016

Glia

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Schwann cell (SC) transplantation following spinal cord injury (SCI) may have therapeutic potential. Functional recovery is limited however, due to poor SC interactions with host astrocytes and the induction of astrogliosis. Olfactory ensheathing cells (OECs) are closely related to SCs, but intermix more readily with astrocytes in culture and induce less astrogliosis. We previously demonstrated that OECs express higher levels of sulfatases, enzymes that remove 6‐O‐sulfate groups from heparan sulphate proteoglycans, than SCs and that RNAi knockdown of sulfatase prevented OEC‐astrocyte mixing in vitro. As human OECs are difficult to culture in large numbers we have genetically engineered SCs using lentiviral vectors to express sulfatase 1 and 2 (SC‐S1S2) and assessed their ability to interact with astrocytes. We demonstrate that SC‐S1S2s have increased integrin‐dependent motility in the presence of astrocytes via modulation of NRG and FGF receptor‐linked PI3K/AKT intracellular signaling and do not form boundaries with astrocytes in culture. SC‐astrocyte mixing is dependent on local NRG concentration and we propose that sulfatase enzymes influence the bioavailability of NRG ligand and thus influence SC behavior. We further demonstrate that injection of sulfatase expressing SCs into spinal cord white matter results in less glial reactivity than control SC injections comparable to that of OEC injections. Our data indicate that sulfatase‐mediated modification of the extracellular matrix can influence glial interactions with astrocytes, and that SCs engineered to express sulfatase may be more OEC‐like in character. This approach may be beneficial for cell transplant‐mediated spinal cord repair. GLIA 2016 GLIA 2017;65:19–33

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Association of cell surface heparan sulfate proteoglycans of Schwann cells with extracellular matrix proteins

Cited by 34 publications

References 38 publications

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

General Strategies for the Characterization of Carbohydrates from Recombinant Glycoprotein Therapeutics

Sulfatase‐mediated manipulation of the astrocyte‐Schwann cell interface

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Association of cell surface heparan sulfate proteoglycans of Schwann cells with extracellular matrix proteins

Cited by 34 publications

References 38 publications

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study *

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study *

General Strategies for the Characterization of Carbohydrates from Recombinant Glycoprotein Therapeutics

Sulfatase‐mediated manipulation of the astrocyte‐Schwann cell interface

Contact Info

Product

Resources

About

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*