Association of circulating VacA-neutralizing antibodies with gastric cancer and duodenal ulcer

Ayala, Guadalupe; Flores-Luna, Lourdes; Hernández-Amaro, Dulce; Mendoza‐Hernández, Guillermo; Chihu-Amparán, Lilia; Bernal-Sahagún, Fernando; Camorlinga, Margarita; Lazcano‐Ponce, Eduardo; Torres, Javier

doi:10.1007/s10552-011-9817-5

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…Chronic colonization of Hp is the major risk factor for GC in both Chinese and Mexican populations (Kamangar et al, 2007; Ayala et al, 2011). However, GC occurs mainly at the cardia of the stomach in Shanxi, China, but in the non-cardia of the stomach in Mexico.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the Human Stomach Microbiota in Gastric Cancer Patients

Torres

et al. 2017

Front. Cell. Infect. Microbiol.

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Helicobacter pylori (Hp) is the primary cause of gastric cancer but we know little of its relative abundance and other microbes in the stomach, especially at the time of gastric cancer diagnosis. Here we characterized the taxonomic and derived functional profiles of gastric microbiota in two different sets of gastric cancer patients, and compared them with microbial profiles in other body sites. Paired non-malignant and tumor tissues were sampled from 160 gastric cancer patients with 80 from China and 80 from Mexico. The 16S rRNA gene V3–V4 region was sequenced using MiSeq platform for taxonomic profiles. PICRUSt was used to predict functional profiles. Human Microbiome Project was used for comparison. We showed that Hp is the most abundant member of gastric microbiota in both Chinese and Mexican samples (51 and 24%, respectively), followed by oral-associated bacteria. Taxonomic (phylum-level) profiles of stomach microbiota resembled oral microbiota, especially when the Helicobacter reads were removed. The functional profiles of stomach microbiota, however, were distinct from those found in other body sites and had higher inter-subject dissimilarity. Gastric microbiota composition did not differ by Hp colonization status or stomach anatomic sites, but did differ between paired non-malignant and tumor tissues in either Chinese or Mexican samples. Our study showed that Hp is the dominant member of the non-malignant gastric tissue microbiota in many gastric cancer patients. Our results provide insights on the gastric microbiota composition and function in gastric cancer patients, which may have important clinical implications.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the Human Stomach Microbiota in Gastric Cancer Patients

Torres

et al. 2017

Front. Cell. Infect. Microbiol.

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133

122

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Serum VacA antibody is associated with risks of peptic ulcer and gastric cancer: A meta-analysis

Liu

Gong

et al. 2016

Microbial Pathogenesis

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vacA s1m1 genotype and cagA EPIYA-ABC pattern are predominant among Helicobacter pylori strains isolated from Mexican patients with chronic gastritis

Atrisco-Morales

Martínez-Santos

Román-Román

et al. 2018

Journal of Medical Microbiology

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PurposeVirulent genotypes of Helicobacter pylori vacA s1m1/cagA+/babA2+ have been associated with severe gastric diseases. VacA, CagA and BabA are polymorphic proteins, and their association with the disease is allele-dependent. The aims of this work were: (i) to determine the prevalence of H. pylori by type of chronic gastritis; (ii) to describe the frequency of cagA, babA2 and vacA genotypes in strains from patients with different types of chronic gastritis; (iii) to characterize the variable region of cagA alleles.MethodologyA total of 164 patients with chronic gastritis were studied. Altogether, 50 H. pylori strains were isolated, and the status of cagA, babA2 and vacA genotypes was examined by PCR. cagA EPIYA segment identification was performed using PCR and sequencing of cagA fragments of six randomly selected strains.Results/Key findingsThe overall prevalence of H. pylori was 30.5 %. Eighty percent of the isolated strains were vacA s1m1, and the cagA and babA2 genes were detected in 74 and 32 % of the strains, respectively. The most frequent genotypes were vacA s1m1/cagA+/babA2- and vacA s1m1/cagA+/babA2+, with 40 % (20/50) and 28 % (14/50), respectively. In cagA+, the most frequent EPIYA motif was -ABC (78.4 %), and EPIYA-ABCC and -ABCCC motifs were found in 10.8 % of the strains. A modified EPIYT-B motif was found in 66.6 % of the sequenced strains.ConclusionH. pylori strains carrying vacA s1m1, cagA+ and babA2- genotypes were the most prevalent in patients with chronic gastritis from the south of Mexico. In the cagA+ strains, the EPIYA-ABC motif was the most common.

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Association of circulating VacA-neutralizing antibodies with gastric cancer and duodenal ulcer

Abstract: This study showed that VacA-neutralizing antibodies are significantly associated with gastroduodenal diseases, especially GC, and that they might be used as risk markers of GC and DU.

Cited by 4 publications

References 42 publications

Molecular Characterization of the Human Stomach Microbiota in Gastric Cancer Patients

Molecular Characterization of the Human Stomach Microbiota in Gastric Cancer Patients

Serum VacA antibody is associated with risks of peptic ulcer and gastric cancer: A meta-analysis

vacA s1m1 genotype and cagA EPIYA-ABC pattern are predominant among Helicobacter pylori strains isolated from Mexican patients with chronic gastritis

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