Association of Deslorelin and Sulpiride for Double Ovulation Induction in Mares

Abstract

The aim of this study was to verify the effect of deslorelin and sulpiride association on double ovulation induction in mares. Ten mares were used for this study. Eight days after ovulation, luteolysis was induced and mares were submitted a daily ultrasonographic evaluation until observation of at least two follicles measuring between 20 and 25 mm. Animals were split in two experimental groups in a crossover study. In deslorelin (DES) group, the animals received 150 mg deslorelin, intramuscularly, every 12 hours, until the second largest follicle reached at least 33 mm in diameter, at which time ovulation induction was performed with 1.500 IU of human chorionic gonadotrophin, IV. In the DES + sulpiride (SULP) group, the treatment was performed in the same manner as in the DES group, associated with the administration of 1 g of sulpiride, orally, once daily until the time of ovulations induction. In both groups, ultrasound examinations were performed daily to evaluate follicular development and the detection of ovulations. Double ovulations were observed in 70% (7/10) of the animals of the DES group and 50% (5/10) of the animals in the DES + SULP group. The percentage of ovulations corresponding to the number of follicles developed was 81.8% (18/22) for the DES group and 79% (15/19) for the DES + SULP group, and no difference was observed between the groups (P > .05). We conclude that association of sulpiride with deslorelin acetate did not contribute to the increase in double ovulations in mares.

Equine Embryo Production by Means of Double Ovulation Protocols in Colombian Creole Mares

“…Which alters the transport of oocytes to the oviduct where fertilization by sperm occurs. Considering the above, the embryo recovery rate increases when two or three ovulations are stimulated per ovary [7]. Which is why in equines, it is not thought in protocols to superovulate but in protocols for double ovulation there is a good embryo recovery and to avoid malformations in the follicles and in the oviduct [5].…”

“…It reaches the pituitary system, causing the secretion of gonadotropins such as follicle stimulating hormone (FSH), which generates the process of follicular growth and maturation, and luteinizing hormone (LH), which is responsible for intervening in the process of ovulation and formation of the corpus luteum (CL) [4]. Estrogen (E2) also stimulates the proliferation of all epithelial and stromal cells of the super cial stratum during estrus (until ovulation), triggering after the hormonal peak, the mare to ovulate between 24 and 48 h before the end of estrus [5] The use of new techniques to increase reproductive e ciency in mares concerning embryo production has been limited, especially due to the lack of reliable protocols for the induction of superovulations [6], which are intended to produce two oocytes per hormonal treatment, which are to be potentially transferable embryos after being fertilized, collected from a genetically superior donor mare and transferred to recipient mares that are responsible for gestation, increasing the number of offspring per female per year [7].…”