Association of detoxification enzymes with butene-fipronil in larvae and adults of Drosophila melanogaster

Abstract

Insecticide resistance is a major challenge in successful insect pest control as the insects have the ability to develop resistance to various widely used insecticides. Butene-fipronil is a novel compound with high toxicity to insects and less toxicity to the non-target organisms. In the present study, the effect of butene-fipronil alone and in combination with three enzyme inhibitors, piperonyl butoxide (PBO), diethyl maleate (DEM), and triphenyl phosphate (TPP), was carried out on larvae and adults of Drosophilia melanogaster. Our results indicated that the co-toxicity indices of butene-fipronil + PBO, butene-fipronil + TPP, and butene-fipronil + DEM mixtures were 437.3, 335.0, and 210.3, respectively, in the second-instar larvae, while 186.6, 256.2, and 238.5, respectively, in the adults, indicating synergistic effects. Interestingly, butene-fipronil increased the expression of CYP28A5 in the larvae; CYP9F2, CYP304A1, CYP28A5, and CYP318A1 in the female adults; and CYP303A1 and CYP28A5 in the male adults. Furthermore, high-level expression of Est-7 was observed in the female adults compared to larvae and male adults. Our results suggest that there is no difference in butene-fipronil metabolism in larvae and male and female adults of D. melanogaster.

RNA interference-mediated knockdown of a cytochrome P450 gene enhanced the toxicity of α-cypermethrin in xanthotoxin-fed larvae of Spodoptera exigua (Hübner)

“…xanthotoxin-fed S. exigua might result from the ability of this plant allelochemical to induce detoxification enzymes, mainly cytochrome P450s. The increase in activity of detoxification enzymes seen here is consistent with previous studies, which showed a similar increase in activity of cytochrome P450s [12,29,32,60] in many polyphagous herbivorous insects exposed to plant secondary metabolites and insecticides. Because the synergist PBO can also inhibit nonspecific esterase activity [61], the effect of xanthotoxin intake on carboxylesterase and Glutathione S-Transferases and its corresponding contribution to the tolerance of pyrethroid insecticide to S. exigua larvae should be investigated in further study.…”

Sub-lethal effects of lufenuron exposure on spotted bollworm Earias vittella (Fab): key biological traits and detoxification enzymes activity

“…Further studies show that different insecticide resistance mechanisms in S. litura, bemisia tabaci and Drosophila melanogaster were associated with the enhanced activity of cytochrome P450 monooxygenase (MFO) and esterase (Young et al 2006;Karuppaiah et al, 2017;Wang et al 2018). Mechanisms involved in P450 monooxygenase and esterase are also observed in butne-fipronil exposed D. melanogaster (Arain et al 2018). To our best knowledge, this is the first study of the sub lethal effects of lufenuron exposure on key biological traits and detoxification enzymes activity.…”

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

“…Although we experimentally determined the interaction between miR‐2b‐3p and cytochrome P450 ( CYP9f2 ), we have not provided direct evidence that CYP9F2 can metabolize deltamethrin or chlorantraniliprole and its role in the detoxification of these compounds in P. xylostella requires further investigation. However, previous studies have shown that the expression levels of CYP9F2 and other members of the CYP9 family increase following application of insecticides and that their transcript levels are significantly higher in resistant insects (Stevens et al ., ; Ghanim & Kontsedalov, ; Li et al ., ; Arain et al ., ). The ability of miR‐2b‐3p to decrease deltamethrin resistance in P. xylostella larvae makes this miRNA a good candidate to produce a transgenic plant for further investigation.…”