Association of Diet With Skin Histological Features in UV-B–Exposed Mice

Abstract

IMPORTANCE Long-term exposure to solar radiation produces deleterious photoaging of the skin. It is not known if diet can influence skin photoaging.OBJECTIVES To study the influence of a calorie-restricted diet and an obesity diet in mice exposed to long-term UV-B irradiation to assess if there is an association between diet and histopathological response to UV-B irradiation. DESIGN, SETTING, AND PARTICIPANTSIn this animal model study in an academic setting, the dorsal skin of SKH1 hairless mice receiving normal, calorie-restricted, and obesity diets was exposed to UV-B irradiation 3 times a week for 10 weeks and were compared with corresponding controls. The mice were placed in the following groups, with 8 animals in each group: (1) intact control (C) with regular diet and no UV-B exposure, (2) intact control with UV-B exposure (CR), (3) calorie-restricted diet (CrC), (4) calorie-restricted diet with UV-B exposure (CrR), (5) obesity diet (OC), and ( 6) obesity diet with UV-B exposure (OR). The experiment was conducted during October through December 2013. Tissue processing and histological analysis were completed in 2016.MAIN OUTCOMES AND MEASURES Histomorphometric analysis was performed on paraffin-embedded skin sections stained by histological and immunohistochemical methods for estimation of epidermal thickness, epidermal proliferating cell nuclear antigen index, collagen I, elastic fibers, fibroblasts, mast cells, dermal cellularity, and adipose layer ratio. Changes in wrinkles were noted. RESULTSHairless female mice (age range, 6-8 weeks) were obtained. With a normal diet, changes from UV-B irradiation occurred in epidermal thickness, epidermal proliferating cell nuclear antigen index, collagen I, elastic fibers, fibroblasts, and mast cells, which were modestly influenced by an obesity diet. Calorie restriction influenced the skin in nonirradiated control animals, with higher values for most variables. After UV-B exposure in animals with calorie restriction, epidermal thickness was increased, but other variables were unaffected. Animals receiving the calorie-restricted diet lost weight when exposed to long-term UV-B irradiation. Wrinkles were reduced in the calorie-restricted control group and in UV-B-exposed animals who received the obesity diet.CONCLUSIONS AND RELEVANCE Dietary alterations seem to modify histopathological responses to UV-B exposure in the skin of hairless mice.LEVEL OF EVIDENCE NA.

The Effects of a Fasting Mimicking Diet on Skin Hydration, Skin Texture, and Skin Assessment: A Randomized Controlled Trial

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“…The mouse skin light model plays a crucial role in exploring skin inflammation, neoplastic lesions, immune response, and other related factors. [110,[113][114][115] This model has found extensive applications in life sciences, medical research, beauty, and other domains.…”

Photochemoprotective Potential of the Ethyl Acetate Fraction from Eugenia hiemalis Leaves and Its 2,6‐Di‐O‐galloylarbutin Isolate Against UVB‐Induced Photodamage

“…Acute and chronic exposure to UV radiation has several effects on skin structure, promoting numerous histological changes, such as epidermal thickening, significant difference from nonirradiated and untreated group (NC); #P < 0.05: significant difference from irradiated and untreated group (IC). F1C, formulation control group (UVB-irradiated and treated with F1, i.e., emulsion without EAF); F2T, F2-treated group (UVB-irradiated and treated with F2; i.e., emulsion that contained 1% EAF) sunburn cell formation, dermal alterations (e.g., collagen degradation) and mast cell activation (49,50). Studies have shown that topical treatment with formulations that contain plant materials rich in phenolic compounds (e.g., ethanolic extract from Eucalyptus globulus leaves and ethyl acetate fraction from N. cuspidata leaves) decreased collagen degradation, in addition to inhibiting the formation of sunburn cells, inhibiting the increase in epidermal thickness and inhibiting the activation of mast cells in UVB-irradiated skin in mice (36,51).…”