Association of Dietary Nitrate Intake with the 15-Year Incidence of Age-Related Macular Degeneration

Gopinath, Bamini; Liew, Gerald; Kifley, Annette; Lewis, Joshua R.; Bondonno, Catherine P.; Joachim, Nichole; Hodgson, Jonathan M.; Mitchell, Paul

doi:10.1016/j.jand.2018.07.012

Cited by 16 publications

(18 citation statements)

References 13 publications

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“…Overall, the data obtained from this study show that the anti-oxidant and anti-apoptotic effects of flavonoids may limit neurodegeneration by providing neurotrophic support to prevent retinal damage in an animal model of autosomal dominant RP (Tvrm4 mice) as in other types of retinal diseases, such as diabetic retinopathy ( Al-Dosari et al, 2017 ) and AMD ( Maccarone et al, 2008 ; Falsini et al, 2010 ; Gopinath et al, 2018 ). In this work, we first used the Tvrm4 mouse model of RP to test the potential beneficial effects of two nutraceutical compounds, naringenin, and quercetin in single or combined administration, found in a typical Western diet, using a sub-chronic non-invasive treatment.…”

Section: Discussionmentioning

confidence: 74%

“…Indeed, these flavonoids show antioxidant properties that interact with the cellular detoxification enzyme system ( Al-Dosari et al, 2017 ). Furthermore, it has been shown that the anti-oxidant and anti-apoptotic effects of flavonoids can limit neurodegeneration by providing neurotrophic support to prevent retinal damage in RP as in other types of retinal disorders, such as diabetic retinopathy ( Al-Dosari et al, 2017 ) and age-related macular degeneration (AMD) ( Maccarone et al, 2008 ; Falsini et al, 2010 ; Gopinath et al, 2018 ). Our findings confirm, and extend, previous data published by other groups on the pivotal role of increased oxidative stress in photoreceptor death and the beneficial effects of antioxidant molecules in slowing retinal degeneration.…”

Section: Discussionmentioning

confidence: 99%

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Nutraceutical Molecules Slow Down Retinal Degeneration, in Tvrm4 Mice a Model of Retinitis Pigmentosa, by Genetic Modulation of Anti-oxidant Pathway

et al. 2022

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Rhodopsin (RHO) mutations are responsible for 25–40% of the dominant cases of retinitis pigmentosa (RP) with different severity and progression rates. The Tvrm4 mice, heterozygous for an I307N dominant mutation of RHO, display a normal retinal phenotype when raised in ambient light conditions, but undergo photoreceptor degeneration when briefly exposed to strong white light. Here, The Tvrm4 mice is pre-treated with naringenin 100 mg/kg/die, quercetin 100 mg/kg/die, naringenin 50 + quercercetin 100 mg/kg/die or vehicle dimethyl sulfoxide (DMSO 0.025%) in the drinking water for 35 days. On the 30th day, retinal degeneration was induced by exposure for 1 min to the white light of 12,000 lux intensity, and the treatment was repeated for another 5 days. At the end of the protocol retinal functionality was tested by recording an electroretinogram (ERG). The retinal tissue was collected and was used for further analyses, including immunohistochemically, biochemical, and molecular biology assays. The data obtained show that treatment with nutraceutical molecules is effective in counteracting retinal degeneration by preserving the functionality of photoreceptors and increasing the antioxidant and anti-apoptotic pathways of retinal cells. The present data confirm that nutraceutical molecules are effective in slowing photoreceptor degeneration in a mutation-independent way by modulating the antioxidant response of the retina at the gene expression level.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Nutraceutical Molecules Slow Down Retinal Degeneration, in Tvrm4 Mice a Model of Retinitis Pigmentosa, by Genetic Modulation of Anti-oxidant Pathway

et al. 2022

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“…Indeed, these flavonoids exhibit anti-oxidant properties interacting with enzymatic system of cellular detoxification through a scavenger’s action (Al-Dosari et al, 2017). Furthermore, it has been demonstrated that the anti-oxidant and anti-apoptotic effects of flavonoids may limit neurodegeneration by providing neurotrophic support to prevent retinal damage in RP like in other kind of retinal disorders such as diabetic retinopathy (Al-Dosari et al, 2017) and Age-Related Macular Degeneration (Gopinath et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

A Nutraceutical Strategy to Slowing Down the Progression of Cone Death in an Animal Model of Retinitis Pigmentosa

et al. 2019

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Retinitis pigmentosa (RP) is an inherited retinal dystrophy characterized by progressive degeneration of the visual cells and abnormalities in retinal pigment epithelium, the vision is lost slowly, and the final outcome is total blindness. RP primarily affects rods, but cones can also be affected as a secondary effect. Photoreceptor cell death is usually triggered by apoptosis, however the molecular mechanisms linking the rod degeneration to the secondary cone death are poorly understood. Possible causes of the secondary cone death are oxidative stress and/ or the release of toxic factors from dying rods. The aim of this study is to analyze the effect of nutraceutical molecules with antioxidant properties, on the progression of the disease in an established animal model of RP, and rd10 mice. We show that chronic treatment per os with a flavanone (naringenin) or a flavonol (quercetin) present in citrus fruits, grapes and apples, preserves retinal morphology, and ameliorates functionality. These actions are associated with a significant reduction of stress-oxidative markers, such as the detoxifying enzymes Sod1 and Sod2. In addition, naringenin and quercetin treatment reduces the levels of acrolein staining associated with a reduction of ROS in the cellular environment. The study demonstrates the beneficial effects of naringenin and quercetin, two molecules that possess antioxidant properties, limiting neurodegeneration, and thus preventing cone damage.

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“…In addition, overfertilization of vegetables like lettuce, spinach, celery, and Chinese cabbage causes nitrate accumulation at levels of up to several g/kg fresh weight [44,45,46,47]. Possible health impacts of a high dietary intake of nitrate are intensively and controversially discussed [48,49,50,51,52,53,54,55].…”

Section: Introductionmentioning

confidence: 99%

Determination of the [15N]-Nitrate/[14N]-Nitrate Ratio in Plant Feeding Studies by GC–MS

et al. 2019

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Feeding experiments with stable isotopes are helpful tools for investigation of metabolic fluxes and biochemical pathways. For assessing nitrogen metabolism, the heavier nitrogen isotope, [15N], has been frequently used. In plants, it is usually applied in form of [15N]-nitrate, which is assimilated mainly in leaves. Thus, methods for quantification of the [15N]-nitrate/[14N]-nitrate ratio in leaves are useful for the planning and evaluation of feeding and pulse–chase experiments. Here we describe a simple and sensitive method for determining the [15N]-nitrate to [14N]-nitrate ratio in leaves. Leaf discs (8 mm diameter, approximately 10 mg fresh weight) were sufficient for analysis, allowing a single leaf to be sampled multiple times. Nitrate was extracted with hot water and derivatized with mesitylene in the presence of sulfuric acid to nitromesitylene. The derivatization product was analyzed by gas chromatography–mass spectrometry with electron ionization. Separation of the derivatized samples required only 6 min. The method shows excellent repeatability with intraday and interday standard deviations of less than 0.9 mol%. Using the method, we show that [15N]-nitrate declines in leaves of hydroponically grown Crassocephalum crepidioides, an African orphan crop, with a biological half-life of 4.5 days after transfer to medium containing [14N]-nitrate as the sole nitrogen source.

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Association of Dietary Nitrate Intake with the 15-Year Incidence of Age-Related Macular Degeneration

Cited by 16 publications

References 13 publications

Nutraceutical Molecules Slow Down Retinal Degeneration, in Tvrm4 Mice a Model of Retinitis Pigmentosa, by Genetic Modulation of Anti-oxidant Pathway

Nutraceutical Molecules Slow Down Retinal Degeneration, in Tvrm4 Mice a Model of Retinitis Pigmentosa, by Genetic Modulation of Anti-oxidant Pathway

A Nutraceutical Strategy to Slowing Down the Progression of Cone Death in an Animal Model of Retinitis Pigmentosa

Determination of the [15N]-Nitrate/[14N]-Nitrate Ratio in Plant Feeding Studies by GC–MS

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