Association of Early Clinical Response to Laser Rejuvenation of Photoaged Skin with Increased Lipid Metabolism and Restoration of Skin Barrier Function

Garza, Luis A.; Sheu, Mary; Kim, Noori; Tsai, Jerry; César, Sabrina Sisto Alessi; Lee, Jianming; Hawkins, Stacy S.; Chien, Anna L.; Kang, Sewon

doi:10.1016/j.jid.2022.07.024

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…6,10 Temporal changes in collagen biosynthesis pathways, matrix metalloproteases, and pro-inflammatory and anti-inflammatory cells and cytokines occur after AFL treatment of skin in a strictly coordinated manner that eventually leads to dermal remodeling. 4,[11][12][13][14] Macrophages are key players in coordinating this healing process due to their plethora of functions. Every phase in the healing is dominated by different macrophage phenotypes.…”

Section: Introductionmentioning

confidence: 99%

“…Studies of the underlying biological dynamics after AFL treatment of skin have suggested that AFL reinvigorates tissue homeostasis by inducing a wound healing response involving remodeling the extracellular matrix 6,10 . Temporal changes in collagen biosynthesis pathways, matrix metalloproteases, and pro‐inflammatory and anti‐inflammatory cells and cytokines occur after AFL treatment of skin in a strictly coordinated manner that eventually leads to dermal remodeling 4,11–14 . Macrophages are key players in coordinating this healing process due to their plethora of functions.…”

Section: Introductionmentioning

confidence: 99%

“…Ablative fractional laser (AFL) is a commonly used noninvasive cosmetic procedure to reduce signs of photoaging. [1][2][3][4] The rationale behind using AFL to improve skin appearance is that the controlled thermal microinjury ablates the skin tissue and creates microscopic treatment zones (MTZ), which initiates a healing process that results in improved skin texture and skin tightening, termed skin rejuvenation. 2,5,6 AFL has also been reported to be an effective treatment for reducing scar tissue due to AFL's favorable esthetic outcomes and acceptable adverse effects.…”

Section: Introductionmentioning

confidence: 99%

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Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages

Wiinberg,

Andresen,

Haedersdal

et al. 2024

Lasers Surg Med

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ObjectivesAblative fractional laser (AFL) treatment is a well‐established method for reducing signs of skin photoaging. However, the biological mechanisms underlying AFL‐induced healing responses and skin rejuvenation remain largely unknown. It is known that macrophages play an important role in orchestrating healing, normalization, and remodeling processes in skin. Macrophage phenotypes are characterized by inflammatory markers, including arginase‐1 (Arg1), major histocompatibility class II molecules (MHC II), and CD206. This study aims to explore AFL's effect on macrophage phenotype by evaluating changes in inflammatory markers and the potential concurrent accumulation of Arg1 in the skin.MethodsMice (n = 9) received a single AFL treatment on the left side of the back skin (100 mJ/microbeam, 5% density) while the right side of the back remained untreated as control. Treated and untreated skin from each mouse were collected Day 5 posttreatment for flow cytometry and histology analysis. Flow cytometry evaluated the immune infiltration of macrophages and the expression of macrophage inflammatory markers (Arg1, MHC II, and CD206). In addition, Arg1 presence in the skin was evaluated through antibody staining of histology samples and quantification was performed using QuPath image analysis software.ResultsFollowing AFL, the number of macrophages increased 11‐fold (p = 0.0053). Phenotype analysis of AFL‐treated skin revealed an increase in the percentage of macrophages positive for Arg1 (p < 0.0001) and a decrease in the percentage of macrophages positive for MHC II (p < 0.0001) compared to untreated skin. No significant differences were observed in percentage of CD206‐positive macrophages (p = 0.8952). Visualization of AFL‐treated skin demonstrated a distinct pattern of Arg1 accumulation that correlated with the microscopic treatment zones (MTZ). Quantification of the percentage of Arg1‐positive area in epidermis and dermis showed a significant increase from 3.5% ± 1.2% to 5.2% ± 1.7 (p = 0.0232) and an increase from 2.2% ± 1.2% to 9.6% ± 3.3 (p < 0.0001) in whole skin samples.ConclusionAFL treatment polarizes macrophages toward a wound healing phenotype and induces Arg1 accumulation in the MTZ. We propose that the polarized wound healing macrophages are a major source for the increased Arg1 levels observed in the skin following treatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages

Wiinberg,

Andresen,

Haedersdal

et al. 2024

Lasers Surg Med

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“…On the basis of Garza and colleagues' report, due to the application of the laser, a robust gene expression signature relative to immune response and double-stranded RNA signaling appeared in the treated participants. 6 Genomics methods such as microarrays, which investigate gene expression alterations, provide a large number of dysregulated genes under the applied condition. 7 There are several microarray experiments relative to laser effects on the skin.…”

Section: Introductionmentioning

confidence: 99%

Collagen Synthesis as a Prominent Process During the Interval between Two Laser Sessions

Mansouri,

Arjmand,

Hamzeloo-Moghadam

et al. 2023

J Lasers Med Sci

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Introduction: Many people suffer from skin photodamage, especially photoaging. The application of a laser to repair damages is a common therapeutic method that is used widely. In the present study, the effectiveness and molecular mechanism of an Er:Glass non-ablative fractional laser on the human skin was assessed via bioinformatics and network analysis. Methods: The gene expression profiles of 17 white female forearm skins which received an Er:Glass non-ablative fractional laser before and after laser treatment in two sessions were extracted from Gene Expression Omnibus (GEO). Data were evaluated via GEO2R and the significant differentially expressed genes (DEGs) were assessed via protein-protein interaction (PPI) network analysis. The central nodes were identified and discussed for the compared set of samples. Results: Five classes of samples were clustered in two categories: first, baseline, 7 and 14 days after the first session of laser treatment, and second, one day after the first laser session, 29 days after the first laser session, and 1 day after the second laser session. The gross cell functions such as cell division and cell cycle and immune response were highlighted as the early affected targets of the laser. Collagen synthesis was resulted after the first laser session. Conclusion: In conclusion, the time interval between laser sessions plays a critical role in the effectiveness of laser therapy. Findings indicate that the gross effect of laser application appears in a short time, and important processes such as collagen synthesis happen later.

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A retrospective study of non-insulated microneedle radiofrequency on wrinkles of facial photoaging subjects

Anwer,

Sun,

Wang

et al. 2024

Lasers Med Sci

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Association of Early Clinical Response to Laser Rejuvenation of Photoaged Skin with Increased Lipid Metabolism and Restoration of Skin Barrier Function

Cited by 4 publications

References 48 publications

Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages

Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages

Collagen Synthesis as a Prominent Process During the Interval between Two Laser Sessions

A retrospective study of non-insulated microneedle radiofrequency on wrinkles of facial photoaging subjects

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Association of Early Clinical Response to Laser Rejuvenation of Photoaged Skin with Increased Lipid Metabolism and Restoration of Skin Barrier Function

Cited by 4 publications

References 48 publications

Ablative fractional CO2 laser treatment promotes wound healing phenotype in skin macrophages

Ablative fractional CO2 laser treatment promotes wound healing phenotype in skin macrophages

Collagen Synthesis as a Prominent Process During the Interval between Two Laser Sessions

A retrospective study of non-insulated microneedle radiofrequency on wrinkles of facial photoaging subjects

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Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages

Ablative fractional CO₂ laser treatment promotes wound healing phenotype in skin macrophages