Association of Endocrine Factors (Insulin-Like Growth Factor-II and Binding Protein-3) with Litter Size in Pigs

Yun, J. S.; Kang, Woojin; Seo, D. S.; Park, S. S.; Lee, C. Y.; Ko, Yongmin

doi:10.5713/ajas.2001.307

Cited by 13 publications

(9 citation statements)

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“…In this context, the greater IGF-II concentration in the high-line vs low-line at d-90 Px may add to the decreased IGF-I activity in the former at this stage if IGF-II could be inhibitory to IGF-I action as has been observed in sheep (Koea et al, 1992). It remains unexplained, however, why the present result on IGF-II was different from that of a previous study (Yun et al, 2001) where the d-90 Px IGF-II concentration was greater in the low-litter size Yorkshire sows than in the high-litter size.…”

Section: Discussioncontrasting

confidence: 56%

“…Lamberson et al (1995Lamberson et al ( , 1996 have reported that circulating IGF-I and IGF-II concentrations of growing gilts had no relation to their subsequent litter size, indicating that prepubertal IGF concentration is not reflected into later reproductive performance. More recently, Yang et al (1999) and Yun et al (2001) have reported that serum IGF-I and IGF-II concentrations were different between low-and highlitter size Yorkshire sows depending on the stage of pregnancy. However, relationships of the periparturient IGF concentration in plasma and the concurrent reproductive performance have not been reported in the latter studies.…”

Section: Introductionmentioning

confidence: 99%

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Relationships of Plasma Insulin-like Growth Factor (IGF)-I and IGF-II Concentrations to Litter Size

Lee

Baik²,

Lee³

et al. 2003

Journal of Animal Science and Technology

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The present study was undertaken to find relationships of plasma insulin-like growth factor (IGF)-I and IGF-II concentrations to litter size and lactation performance. Sixty pure-bred Landrace and Yorkshire pigs having similar farrowing weeks which had been selected from a large number of pregnant gilts and sows were divided into low-(< 0.5 SD) and high-litter size (> + 0.5 SD) lines under a 2 (breed) × 2 (line) factorial arrange of treatments. After adjusting the litter size to nine piglets per sow at farrowing, total litter weight was measured at three weeks postpartum at weaning as an index of milk yield. Blood samples were obtained from the jugular vein at day (d)-90 pregnancy (Px) and at d-15 postpartum. The litter size or the number of piglets born during the present experiment and the average litter size during the entire parities up to the present one were greater in the high-line than in the low-line by 3.7 and 2.4 piglets, respectively (P<0.01); effect of the breed on litter size was not significant. Plasma IGF-II concentration at d-90 Px was greater in the high-line than in the low-line. Litter size and d-90 Px IGF-I concentration were negatively correlated in Landrace (r= 0.46; P<0.05) and tended to be negatively correlated in Yorkshire (r= 0.31; P=0.09), which resulted in a significant negative correlation between these two variables in total animals (r= 0.35; P<0.01). Litter weight at weaning was not different between the two breeds or lines. Relationships between the litter weight and IGF concentration were not consistent across the breed × physiological stage combinations. Results suggest that d-90 Px IGF concentrations may be indicative of the litter size at impending farrowing.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Relationships of Plasma Insulin-like Growth Factor (IGF)-I and IGF-II Concentrations to Litter Size

Lee

Baik²,

Lee³

et al. 2003

Journal of Animal Science and Technology

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“…Relative abundance of IGFBPs with different molecular masses contained in the CCM was determined by WLB (Hossenlopp et al, 1986) as previously described (Lee et al, 1991;Lee and Rechler, 1995;Yun et al, 2001). Twenty microliters of the 10-fold concentrated CCM were electrophoresed on each lane of the SDS-12.5% polyacrylamide gel, and separated proteins were transferred onto nitrocellulose membranes.…”

Section: Western Ligand Blotting (Wlb) Of Igfbpsmentioning

confidence: 99%

Expression and Secretion of the Insulin-like Growth Factor System Components by Pig Liver Cells

Kim

Jin

Baik

et al. 2008

Asian Australas. J. Anim. Sci

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The aim of the present study was to delineate the expression and secretion of insulin-like growth factor (IGF) system components by pig liver cells. Hepatocytes were prepared from 3-wk-old weanling piglets following a two-step collagenase perfusion procedure, after which the cells were incubated for 24 or 48 h at a density of 2×10 5 cells per 35-mm dish in 2-ml Williams' medium E. The cells were found to express the genes encoding IGF-I, IGF-binding proteins (IGFBPs)-2 and -3 and acid-labile subunit (ALS) by reverse transcription-polymerase chain reaction (RT-PCR) following the culture. However, IGF-I was localized to hepatocytes by immunohistochemical analysis, whereas IGFBP-3 was localized to endothelial cells, but not to hepatocytes. This indicated that the IGFBP-3 gene expression detected by RT-PCR was likely to have been contributed by unidentified non-parenchymal cells that had not been removed during the hepatocyte preparation. The conditioned culture medium (CCM) of the cells contained immunoreactive IGF-I and IGF-II, with the latter being seven-fold more abundant than the former. The CCM also contained 43-, 40-, 34-, 31-kDa doublet and 26-kDa IGFBPs as examined by Western ligand blotting. The 40-, 34-and 31-kDa doublet IGFBPs were approximately three-fold as abundant as the 43-and 26-kDa IGFBPs. Moreover, the 43-and 40-kDa doublet and the 34-kDa IGFBPs were immunoprecipitable with IGFBP-3 and IGFBP-2 antibodies, respectively. Overall, these results are similar to those known in the rat, which suggests that the IGF system components are likely to be expressed and secreted in pig liver in a manner similar to that in rat liver.

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“…Serum concentrations of IGF-I and -II were determined by corresponding radioimmunoassays [ 25,26] using commercial antisera (GroPep) following removal of IGF-binding proteins by the acidethanol extraction procedure [27] . Sensitivities of the IGF-I and -II radioimmunoassays were 15.6 and 156 pg, respectively.…”

Section: Determination Of Serum Concentrations Of Glucose and Igf-i Amentioning

confidence: 99%

Effects of Oral Administration of Insulin-Like Growth Factor-I on Circulating Concentration of Insulin-Like Growth Factor-I and Growth of Internal Organs in Weanling Mice

Kim¹,

Ryu²,

Seo³

et al. 2006

Neonatology

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Insulin-like growth factor (IGF)-I is a polypeptide that mediates the growth-promoting action of growth hormone in postnatal animals. The present study was conducted to examine whether orally administered IGF-I would be absorbed into the general circulation and also whether ingested IGF-I would enhance the growth of whole body as well as internal organs, and tissues in 3-week-old ICR-strain female weanling mice. In experiment (Exp) 1, a total of 70 mice received IGF-I orally at 1 µg·g^–1 in 0.2-ml PBS or the vehicle alone. Concentrations of IGF-I and glucose in heart blood were measured after killing 5 animals in each group every fourth hour during a 24-hour period. In Exp 2, a total of 40 mice received oral IGF-I administration at 1 µg·g^–1 or vehicle every third day beginning from day 0 for a 13-day period. Half the animals were killed at day 7 and the other half at day 13. Weights of whole body and organs/tissues (small intestine, liver, thigh muscle, and brain) were measured every day and at slaughter, respectively. In Exp 1, following the oral IGF-I administration, serum IGF-I concentration increased at hour 4 (p < 0.01) and returned to the hour 0 level by hour 8, whereas glucose concentration was lowest at hour 4 and returned to the hour 0 level by hour 16. In the PBS-fed group, neither IGF-I nor glucose concentration changed during the 24-hour period. In Exp 2, weight of small intestine increased (p < 0.05) in response to the oral IGF-I, whereas weights of liver and thigh muscle of the IGF-I-fed group were greater (p < 0.01) and tended to be greater (p = 0.06), respectively, than those of the PBS-fed only at day 13. However, brain weight and serum concentrations of IGF-I and IGF-II were not affected by oral IGF-I administration. Results suggest that although orally administered IGF-I mainly acts at the intestine, a portion of ingested IGF-I is absorbed into the general circulation to enhance the growth of selective organs/tissues in weanling mice.

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Association of Endocrine Factors (Insulin-Like Growth Factor-II and Binding Protein-3) with Litter Size in Pigs

Cited by 13 publications

References 0 publications

Relationships of Plasma Insulin-like Growth Factor (IGF)-I and IGF-II Concentrations to Litter Size

Relationships of Plasma Insulin-like Growth Factor (IGF)-I and IGF-II Concentrations to Litter Size

Expression and Secretion of the Insulin-like Growth Factor System Components by Pig Liver Cells

Effects of Oral Administration of Insulin-Like Growth Factor-I on Circulating Concentration of Insulin-Like Growth Factor-I and Growth of Internal Organs in Weanling Mice

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