Association of Fas/Apo1 gene promoter (-670 A/G) polymorphism in Tunisian patients with IBD

Aleya, Walid Ben; Sfar, Imen; Mouelhi, Leïla; Aouadi, Houda; Makhlouf, M.; Ayed-Jendoubi, Salwa; Matri, Samira; Filali, Azza; Najjar, Taoufik; Abdallah, T. Ben; Ayed, K.; Gorgi, Yousr

doi:10.3748/wjg.15.3643

Cited by 5 publications

(2 citation statements)

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“…FASLG has been proven to be involved in the induction of apoptosis triggered by binding to FAS [ 105 ]. Members of the TNF family have been widely reported to participate in IBDs by regulating the apoptosis of specific local cells [ 106 , 107 ].…”

Section: Resultsmentioning

confidence: 99%

Identification of Candidate Genes Related to Inflammatory Bowel Disease Using Minimum Redundancy Maximum Relevance, Incremental Feature Selection, and the Shortest-Path Approach

Yuan

Zhang

Kong

et al. 2017

BioMed Research International

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Identification of disease genes is a hot topic in biomedicine and genomics. However, it is a challenging problem because of the complexity of diseases. Inflammatory bowel disease (IBD) is an idiopathic disease caused by a dysregulated immune response to host intestinal microflora. It has been proven to be associated with the development of intestinal malignancies. Although the specific pathological characteristics and genetic background of IBD have been partially revealed, it is still an overdetermined disease and the blueprint of all genetic variants still needs to be improved. In this study, a novel computational method was built to identify genes related to IBD. Samples from two subtypes of IBD (ulcerative colitis and Crohn's disease) and normal samples were employed. By analyzing the gene expression profiles of these samples using minimum redundancy maximum relevance and incremental feature selection, 21 genes were obtained that could effectively distinguish samples from the two subtypes of IBD and the normal samples. Then, the shortest-path approach was used to search for an additional 20 genes in a large network constructed using protein-protein interactions based on the above-mentioned 21 genes. Analyses of the 41 genes obtained indicate that they are closely associated with this disease.

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Section: Resultsmentioning

confidence: 99%

Identification of Candidate Genes Related to Inflammatory Bowel Disease Using Minimum Redundancy Maximum Relevance, Incremental Feature Selection, and the Shortest-Path Approach

Yuan

Zhang

Kong

et al. 2017

BioMed Research International

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“…Associations between the A/G −670 Fas polymorphism and autoimmune diseases, such as type 1 diabetes, multiple sclerosis, Sjögren's syndrome, rheumatoid arthritis (RA), and SLE, have been described [ 19 , 33 , 34 , 46 ]. Nevertheless, it remains unclear how this polymorphism participates in LN pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Soluble Fas and the −670 Polymorphism of Fas in Lupus Nephritis

Bollain-y-Goytia

Arellano-Rodríguez

Torres-del-Muro

et al. 2014

International Journal of Nephrology

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This study was performed to clarify the role of soluble Fas (sFas) in lupus nephritis (LN) and establish a potential relationship between LN and the −670 polymorphism of Fas in 67 patients with systemic lupus erythematosus (SLE), including a subset of 24 LN patients with proteinuria. Additionally, a group of 54 healthy subjects (HS) was included. The allelic frequency of the −670 polymorphism of Fas was determined using PCR-RFLP analysis, and sFas levels were assessed by ELISA. Additionally, the WT-1 protein level in urine was measured. The Fas receptor was determined in biopsies by immunohistochemistry (IHC) and in situ hybridization (FISH) and apoptotic features by TUNEL. Results. The −670 Fas polymorphism showed that the G allele was associated with increased SLE susceptibility, with an odds ratio (OR) of 1.86. The sFas was significantly higher in LN patients with the G/G genotype, and this subgroup exhibited correlations between the sFas level and proteinuria and increased urinary WT-1 levels. LN group shows increased expression of Fas and apoptotic features. In conclusion, our results indicate that the G allele of the −670 polymorphism of Fas is associated with genetic susceptibility in SLE patients with elevated levels of sFas in LN with proteinuria.

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