Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis

Brajović, Gavrilo; Stefanović, G; Ilić, Vesna; Petrović, Sonja; Stefanović, Neda; Nikolić-Jakoba, Nataša; Milosević-Jovcić, N

doi:10.1902/jop.2010.100053

Cited by 11 publications

(5 citation statements)

References 31 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although complement activation has been described as an important mechanism in tissue damage in periodontal and periimplant disease (209,210), the most accepted interpretation of its activation is the capacity of the periodontopathogens that use it as a mechanism for tissue degradation in favor of the release of nutritional components, and thus the maintenance of biofilms (211,212) and not directly as a mechanism of tissue damage.…”

Section: Relevance Of Auto-antibodies In the Autoimmune Damagementioning

confidence: 99%

Oral Dysbiosis and Autoimmunity: From Local Periodontal Responses to an Imbalanced Systemic Immunity. A Review

et al. 2020

View full text Add to dashboard Cite

The current paradigm of onset and progression of periodontitis includes oral dysbiosis directed by inflammophilic bacteria, leading to altered resolution of inflammation and lack of regulation of the inflammatory responses. In the construction of explanatory models of the etiopathogenesis of periodontal disease, autoimmune mechanisms were among the first to be explored and historically, for more than five decades, they have been described in an isolated manner as part of the tissue damage process observed in periodontitis, however direct participation of these mechanisms in the tissue damage is still controversial. Autoimmunity is affected by genetic and environmental factors, leading to an imbalance between the effector and regulatory responses, mostly associated with failed resolution mechanisms. However, dysbiosis/infection and chronic inflammation could trigger autoimmunity by several mechanisms including bystander activation, dysregulation of toll-like receptors, amplification of autoimmunity by cytokines, epitope spreading, autoantigens complementarity, autoantigens overproduction, microbial translocation, molecular mimicry, superantigens, and activation or inhibition of receptors related to autoimmunity by microorganisms. Even though autoreactivity in periodontitis is biologically plausible, the associated mechanisms could be related to non-pathologic responses which could even explain non-recognized physiological functions. In this review we shall discuss from a descriptive point of view, the autoimmune mechanisms related to periodontitis physio-pathogenesis and the participation of oral dysbiosis on local periodontal autoimmune responses as well as on different systemic inflammatory diseases.

show abstract

Section: Relevance Of Auto-antibodies In the Autoimmune Damagementioning

confidence: 99%

Oral Dysbiosis and Autoimmunity: From Local Periodontal Responses to an Imbalanced Systemic Immunity. A Review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…1). This might be attributable to a shift toward hypogalactosylated glycoforms occurring during the process of inflammation of the gingiva or significantly less galactose in its sugar chains with immunoglobulin G from advanced CP 50,51 . These findings suggest that inflammation may play a more important role in periodontal tissues than diabetic status.…”

Section: Discussionmentioning

confidence: 89%

“…This might be attributable to a shift toward hypogalactosylated glycoforms occurring during the process of inflammation of the gingiva or significantly less galactose in its sugar chains with immunoglobulin G from advanced CP. 50,51 These findings suggest that inflammation may play a more important role in periodontal tissues than diabetic status. Interestingly, a recent review of the cytokine profiles in the GCF of patients with CP presenting type 2 DM demonstrated that the GCF cytokine profile in patients with and without DM seems to be governed by the intensity of periodontal inflammation, and the role of DM in this regard is rather secondary.…”

Section: Discussionmentioning

confidence: 94%

Diabetes‐Associated Periodontitis Molecular Features in Infrared Spectra of Gingival Crevicular Fluid

Xiang

Duarte

Lima

et al. 2013

Journal of Periodontology

View full text Add to dashboard Cite

Background: It has been established previously that infrared spectroscopy (IRS) can be used to identify periodontitis‐specific molecular signatures in gingival crevicular fluid (GCF) and to confirm clinical diagnoses. This follow‐up study is designed to assess whether this novel technique is also able to differentiate diseased from healthy sites in patients with diabetes mellitus (DM) by analyzing the molecular fingerprints embedded in the GCF. Methods: A total of 65 patients with DM with moderate‐to‐severe chronic periodontitis (CP) was recruited, and 15 individuals without DM (65 sites) without periodontal diseases were used as control. Clinical examination and GCF samples were taken from a total of 351 sites, including periodontitis (109), gingivitis (115), and healthy (127) sites. Corresponding absorption spectra of GCF samples were acquired and processed, and the relative contributions of key functional groups in the infrared spectra were identified and analyzed. The qualitative assessment of clinical relevance of these GCF spectra was interpreted with multivariate statistical analysis: linear discriminant analysis (LDA). Results: Spectral analysis revealed several molecular signatures representing vibrations in protein (amide I and II), lipid ester, and sugar moieties in the GCF of patients with DM with CP and non‐DM controls. The diagnostic accuracy for distinction between healthy and CP sites in patients with DM determined by LDA of GCF spectra was 95.3% for the training set of samples and 87.5% for the validation set. Additional LDA of GCF spectra from healthy sites of non‐DM controls and patients with DM revealed 100% diagnostic accuracy for the training set and 86.7% for the validation set. The regions robotically selected by LDA for the two analyses were slightly different in that first LDA identified major regions clustered with the side chain vibrations originating from protein and DNA contents, whereas the second was predominantly the glycation and protein components. Conclusion: IRS is a feasible method to differentiate disease‐specific molecular signatures in GCF in the presence of DM and to generate a complex biochemical profile of GCF to identify DM‐specific spectral features.

show abstract

“…The relative content of immunoglobulin classes (i.e., immunoglobulin index) for every individual CIC was assessed by dot blot protocols from our previous papers [ 47 , 48 , 49 ]. The CIC IgA, IgG, and IgM levels were determined using peroxidase-conjugated goat anti-human IgA, IgG, and IgM antibodies (Boster Biological Technology Co., Ltd., Pleasanton, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients

Djukić,

Drvenica,

Kovačić

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.

show abstract

Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis

Cited by 11 publications

References 31 publications

Oral Dysbiosis and Autoimmunity: From Local Periodontal Responses to an Imbalanced Systemic Immunity. A Review

Oral Dysbiosis and Autoimmunity: From Local Periodontal Responses to an Imbalanced Systemic Immunity. A Review

Diabetes‐Associated Periodontitis Molecular Features in Infrared Spectra of Gingival Crevicular Fluid

Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients

Contact Info

Product

Resources

About