Association of functional superoxide gene polymorphism with chlamydia trachomatis-associated recurrent spontaneous abortion

Ray, Ankita; Bhati, Tanu; Arora, Renu; Parvez, Suhel; Rastogi, Sangita

doi:10.1007/s11033-023-08405-5

Cited by 2 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reactive oxygen species (ROS) are closely associated with a number of physiological and pathological functions and may influence the function of trophoblast and outcome of pregnancy (Dos Anjos Cordeiro et al, 2023;Ye et al, 2023). More recently, Ray et al reported that the single nucleotide polymorphisms and expression level of SOD1 and SOD2 are associated with C. trachomatisinfected recurrent spontaneous abortion RSA (Ray et al, 2022(Ray et al, , 2023. These studies indicate that SOD1 may be an important potential factor related to RSA.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

Ji,

Jiao,

Zhang

et al. 2024

Physiological Reports

View full text Add to dashboard Cite

Recurrent spontaneous abortion (RSA) is a serious condition that adversely affects women's health. Differentially expressed proteins (DEPs) in plasma of patients experiencing RSA is helpful to find new therapeutic targets and identified with mass spectrometry. In 57 DEPs, 21 were upregulated and 36 were downregulated in RSA. Gene ontology analyses indicated that identified DEPs were associated with cell proliferation, including significantly downregulated insulin‐like growth factor binding protein 2 (IGFBP2). Immunohistochemical result using clinical decidual tissues also showed that IGFBP2 expression was significantly decreased in RSA trophoblasts. Cell proliferation assay indicated that IGFBP2 treatment increased the proliferation and mRNA expressions of PCNA and Ki67 in trophoblast cells. Transcriptome sequencing experiments and Kyoto Encyclopedia of Genes and Genomes analyses revealed that gene expression for components in PI3K‐Akt pathway in trophoblasts was significantly upregulated following IGFBP2 treatment. To confirm bioinformatics findings, we did cell‐based experiments and found that treatment of inhibitors for insulin‐like growth factor (IGF)‐1 receptor‐PI3K‐Akt pathway significantly reduced IGFBP2‐induced trophoblast cell proliferation and mRNA expressions of PCNA and Ki67. Our findings suggest that IGFBP2 may increase trophoblast proliferation through the PI3K‐Akt signaling pathway to affect pregnancy outcomes and that IGFBP2 may be a new target for future research and treatment of RSA.

show abstract

Section: Discussionmentioning

confidence: 97%

“…More recently, Ray et al. reported that the single nucleotide polymorphisms and expression level of SOD1 and SOD2 are associated with C. trachomatis‐infected recurrent spontaneous abortion RSA (Ray et al., 2022 , 2023 ). These studies indicate that SOD1 may be an important potential factor related to RSA.…”

Section: Discussionmentioning

confidence: 99%