Association of Genetic Polymorphisms in TNFRSF11 with the Progression of Genetic Susceptibility to Gastric Cancer

Abstract

Objective. To investigate the relationship between polymorphism of TNFRSF11 gene rs9533156 and rs2277438 and susceptibility to gastric cancer. Methods. A case-control study was conducted to select 577 cases of primary gastric cancer and 678 cases of normal control. We extracted whole blood genomic DNA and amplified the target gene fragment by PCR. The genotyping and allele were tested through the snapshot method. Results. In this case-control study, we observed that there was a difference in the genotype distribution of TNFRSF11 gene rs9533156 between the case group and the control group. The frequency distribution of TC heterozygous mutation in the case group was higher than that in the control group. The smoking rate in the case group (34.49%) was higher than that in the control group (27.29%), and the difference in frequency distribution between the two groups was statistically significant (P=0.006). Our findings suggest that TNFRSF11 rs9533156 is associated with susceptibility to GC, which is more evident among elderly patients (>62 years), nonsmokers, and patients who do not consume alcohol. The analysis of the relationship between the TNFSF11 gene rs9533156 site variant and clinical factors of gastric cancer showed that, compared with the tumor size <2 cm group, patients with tumor size ≥2 cm and whom carrying rs9533156 site mutations had a higher frequency distribution, and the difference was statistically significant (P=0.022). Compared with the nonhyperglycemic group, the frequency distribution of patients with rs9533156 site mutations in the diabetes group was higher, and the difference was statistically significant (P<0.001). Conclusion. This study shows that there is a correlation between smoking and the occurrence of gastric cancer. Based on our research, the functional SNP TNFRSF11 TC genotype may be an indicator of individual susceptibility to GC. The mutation at rs9533156 may be related to the size of gastric cancer. The mutation rate of rs9533156 of TNFSF11 gene is higher in diabetic gastric cancer patients.

Case-control study on CYP4B1 gene polymorphism and susceptibility to gastric cancer in the chinese Han population

“…Previous studies have shown that risk of GC is higher in smokers and drinkers when compared to nosmokers and no-drinkers [19][20][21][22][23]. However, studies have also found that non-drinkers [24] and non-smokers are still at the risk of developing GC [4]. Our results manifested that there was a correlation between the lifestyle of non-smokers and non-drinkers and the risk of GC.…”

“…In recent years, the study of SNP has become an effective tool and method for molecular genetics research, and has attracted much attention [16]. In our study, three SNPs (rs2297810, rs4646491 and rs2297809) of the CYP4B1 gene were taken as the research objects to explore the relationship between them and GC risk.…”

“…Gastric cancer (GC) is the fifth most common cancer and the fourth leading cause of cancer death globally, according to the International Agency for Research on Cancer (IARC) in 2020 [1,2]. Statistically, due to insignificant early symptoms, over 50% of GC patients are in advanced stage when diagnosed [3][4][5]. Modern research shows that the progression of GC is complex and diverse, and multiple factors contribute to its onset.…”

Identifying metastatic biomarkers of colorectal cancer: Machine learning modeling and experimental validation

“…TNFRSF11 (RANK) was rst found to have a role in bone dissolution and lymph node formation, majorly via the RANK/RANKL/OPG pathway [52]. current studies have proved the critical role of RANK/RANKL/OPG in cell migration and invasion.…”