“…Understanding the distribution of ARGs within the gut microbiota of giant pandas, as well as identifying the microbial hosts, is pivotal for formulating effective strategies to curtail antibiotic resistance. Contemporary literature underscores the multifaceted influences on the ARGs present in the gut microbiome, with variables such as age, dietary patterns, seasonal variations, habitat characteristics, geographical location, and management practices being of particular importance [5, 7, 9–12]. Although previous research employing metagenomic sequencing has predominantly focused on the distribution and key drivers affecting ARGs in giant pandas, there remains considerable scope to delve deeper.…”