Association of genetic variants located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes with type 2 Diabetes Mellitus in Bangladeshi population

Abstract

Background Genome-Wide Association Studies have identified genetic polymorphisms located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes on chromosome 3 as susceptibility loci for type 2 diabetes mellitus (T2DM) in different population. The present study investigates the association of these two susceptibility loci (rs11708067 at the ADCY5 and rs11926707 at the PTH1R gene) with T2DM in Bangladeshi population. Furthermore, the association of circulating levels of vitamin D, Calcium (Ca), phosphate, and parathyroid hormone (PTH) with T2DM and the different genotypes of these SNPs were also studied.Methods and Materials A total of 415 individuals (211 T2DM patients and 204 healthy controls) were enrolled in the current study. The rs11708067 and rs11926707 were genotyped using the Taqman SNP Genotyping Assays. Univariate logistic regression analyses were performed for parameter associations with T2DM.Results In a sex-stratified analysis, the variant allele of the ADCY5 SNP (rs11708067) was associated to T2DM in males (odds ratio = 2.5, p-value = 0.02) but PTH1R SNP (rs11926707) did not show any significant correlation with T2DM (odds ratio 1.0 in both sexes, p-value > 0.05). In the correlation study, phosphate was shown to be significantly linked with Ca (p-value < 0.001) and PTH (p-value < 0.05) in both T2DM and healthy individuals. Lastly, neither SNP revealed any link between vitamin D, calcium, nor phosphate levels, with the exception of PTH.Conclusion Although ADCY5 has been identified as a T2DM susceptible locus in the Bangladeshi male population, the same cannot be said for the PTH1R locus in either males or females.