Association of GHR Polymorphisms with Milk Production in Buffaloes

El-Komy, Shymaa M; Saleh, Ayman A.; Abdel-Hamid, Tamer M.; El-Magd, Mohammed A.

doi:10.3390/ani10071203

Cited by 17 publications

(14 citation statements)

References 43 publications

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“…Unlike GECs, SCs are easily extracted from milk samples in forms of pellets. They were also effectively utilized to assess the expression of some milk performance-related genes and proteins in buffalo and goat [ 14 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…Single strand conformation polymorphism (SSCP) and sequencing followed by statistical association analysis were successfully used to detect single nucleotides polymorphisms (SNPs) associated with production, fertility, growth, and milk traits in animals [ 9 , 10 , 11 , 12 , 13 , 14 ]. In ruminants, the majority of PRLR SNPs were detected in E3 and E10.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

El-Magd

Fathy

Kahilo

et al. 2021

Animals

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Prolactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding PRLR genetic diversity and association studies with milk traits in Egyptian water buffaloes. Therefore, the present study was conducted to search for mutations in PRLR and determine their associations with milk performance in these animals. Exon3 (E3) and E10 of PRLR were screened for polymorphisms using single strand conformation polymorphism (SSCP) and sequencing in 400 buffaloes. The associations between haplotypes and milk production (fat%, protein%, lactose%, and solid%) traits as well as mRNA and protein levels of PRL and PRLR were studied. Two single nucleotide polymorphisms (SNPs) in E10 were detected: g.11685G>A (p.Ala494Thr) and g.11773T>C (p.Val523Aal). The G and T alleles were wild (ancestral) alleles, while the A and C alleles were mutant alleles. These SNPs resulted in four haplotypes; AC, AT, GC, and GT. Buffaloes with wild GT haplotypes showed significantly higher milk yield, fat% and protein%, mRNA and protein levels of PRL and PRLR in milk somatic cells than other animals. Animals carrying mutant AC haplotype had inferior milk traits and lowest levels of associated mRNAs and proteins. With these results, we could conclude that the selection of buffaloes with wild GT haplotypes for g.11685G>A and g.11773T>C SNPs of the PRLR gene might improve the milk production traits of Egyptian water buffaloes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

El-Magd

Fathy

Kahilo

et al. 2021

Animals

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“…Genetic variations in DGAT1, MUC1, INSIG2, and GHR in both river and swamp buffaloes were also associated with milk components, milk yield, and mastitis resistance, which are potential candidates for genetic selection (Deng et al, 2016;Li et al, 2018;da Rosa et al, 2020;El-Komy et al, 2020).…”

Section: Comparisons Between River and Swamp Buffaloesmentioning

confidence: 98%

Opportunities and Challenges for Improving the Productivity of Swamp Buffaloes in Southeastern Asia

et al. 2021

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The swamp buffalo is a domesticated animal commonly found in Southeast Asia. It is a highly valued agricultural animal for smallholders, but the production of this species has unfortunately declined in recent decades due to rising farm mechanization. While swamp buffalo still plays a role in farmland cultivation, this species’ purposes has shifted from draft power to meat, milk, and hide production. The current status of swamp buffaloes in Southeast Asia is still understudied compared to its counterparts such as the riverine buffaloes and cattle. This review discusses the background of swamp buffalo, with an emphasis on recent work on this species in Southeast Asia, and associated genetics and genomics work such as cytogenetic studies, phylogeny, domestication and migration, genetic sequences and resources. Recent challenges to realize the potential of this species in the agriculture industry are also discussed. Limited genetic resource for swamp buffalo has called for more genomics work to be done on this species including decoding its genome. As the economy progresses and farm mechanization increases, research and development for swamp buffaloes are focused on enhancing its productivity through understanding the genetics of agriculturally important traits. The use of genomic markers is a powerful tool to efficiently utilize the potential of this animal for food security and animal conservation. Understanding its genetics and retaining and maximizing its adaptability to harsher environments are a strategic move for food security in poorer nations in Southeast Asia in the face of climate change.

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“…Furthermore, in Mediterranean river buffalo females, the C > T substitution in STAT5A was associated with milk protein percentage [ 118 ]. El-Komy et al [ 119 ] screened the GHR polymorphisms and their potential association with milk yield traits in Egyptian buffaloes (400 animals). The mutations in four exons (E4–E6 and E8) of the GHR gene were investigated, and in E4 no variation was detected, while two SNPs in E5 (c.380G > A/p.Arg127Lys and c.387C > T/p.Gly129), a single silent alteration (c.435A > G/p.Pro145) in E6, and an additional missense mutation (c.836T > A/p.Phe279Tyr) in E8 were spotted.…”

Section: Identification Of Genes Affecting Important Buffalo Traitmentioning

confidence: 99%

“…Two SNPs c.380G > A and c.836T > A in the extracellular and transmembrane regions, respectively, were related with milk yield; protein percentage; fat percentage; and 305-day milk, protein, and fat yield, with higher levels in individuals possessing the mutant A allele. Remarkably, the animal with two mutant alleles (AA) gave a higher milk yield, with higher protein and fat percentages, by upregulating the expression of GH , GHR , prolactin ( PRL ), prolactin receptor ( PRLR ), diacylglycerol acyltransferase-1 ( DGAT1 ), CSN2 gene-encoded beta-casein and insulin-like growth factor 1 ( IGF1 ) genes, and proteins in milk-producing cells [ 119 ]. In the CSN1S2 gene, the coding sequence of river and swamp buffaloes, 13 SNPs were identified, including 8 non-synonymous substitutions.…”

Section: Identification Of Genes Affecting Important Buffalo Traitmentioning

confidence: 99%

Whole-Genome Sequencing and Characterization of Buffalo Genetic Resources: Recent Advances and Future Challenges

Rehman

Hassan

Luo

et al. 2021

Animals

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The buffalo was domesticated around 3000–6000 years ago and has substantial economic significance as a meat, dairy, and draught animal. The buffalo has remained underutilized in terms of the development of a well-annotated and assembled reference genome de novo. It is mandatory to explore the genetic architecture of a species to understand the biology that helps to manage its genetic variability, which is ultimately used for selective breeding and genomic selection. Morphological and molecular data have revealed that the swamp buffalo population has strong geographical genomic diversity with low gene flow but strong phenotypic consistency, while the river buffalo population has higher phenotypic diversity with a weak phylogeographic structure. The availability of recent high-quality reference genome and genotyping marker panels has invigorated many genome-based studies on evolutionary history, genetic diversity, functional elements, and performance traits. The increasing molecular knowledge syndicate with selective breeding should pave the way for genetic improvement in the climatic resilience, disease resistance, and production performance of water buffalo populations globally.

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Association of GHR Polymorphisms with Milk Production in Buffaloes

Cited by 17 publications

References 43 publications

Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

Opportunities and Challenges for Improving the Productivity of Swamp Buffaloes in Southeastern Asia

Whole-Genome Sequencing and Characterization of Buffalo Genetic Resources: Recent Advances and Future Challenges

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