Association of Growth Substrates and Bacterial Genera with Benzo[<i>a</i>]pyrene Mineralization in Contaminated Soil

Jones, Maiysha D.; A., Rodgers-Vieira, Elyse; Hu, Jing; Aitken, Michael D.

doi:10.1089/ees.2014.0275

Cited by 35 publications

(11 citation statements)

References 55 publications

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“…In previous studies we have similarly seen that the major degraders of a PAH in the bioreactor are not the same as found from SIP of the untreated soil (30,57). These findings suggest that SIP with an untreated soil will not necessarily predict that the identified organism(s) will be predominant degraders of the same compound under bioremediation conditions.…”

Section: Discussionmentioning

confidence: 52%

Identification of Anthraquinone-Degrading Bacteria in Soil Contaminated with Polycyclic Aromatic Hydrocarbons

Zhang

Adrion

et al. 2015

Appl Environ Microbiol

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Quinones and other oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) are toxic and/or genotoxic compounds observed to be cocontaminants at PAH-contaminated sites, but their formation and fate in contaminated environmental systems have not been well studied. Anthracene-9,10-dione (anthraquinone) has been found in most PAH-contaminated soils and sediments that have been analyzed for oxy-PAHs. However, little is known about the biodegradation of oxy-PAHs, and no bacterial isolates have been described that are capable of growing on or degrading anthraquinone. PAH-degrading Mycobacterium spp. are the only organisms that have been investigated to date for metabolism of a PAH quinone, 4,5-pyrenequinone. We utilized DNA-based stable-isotope probing (SIP) with [U-13 C]anthraquinone to identify bacteria associated with anthraquinone degradation in PAH-contaminated soil from a former manufactured-gas plant site both before and after treatment in a laboratory-scale bioreactor. SIP with [U-13 C]anthracene was also performed to assess whether bacteria capable of growing on anthracene are the same as those identified to grow on anthraquinone. Organisms closely related to Sphingomonas were the most predominant among the organisms associated with anthraquinone degradation in bioreactor-treated soil, while organisms in the genus Phenylobacterium comprised the majority of anthraquinone degraders in the untreated soil. Bacteria associated with anthracene degradation differed from those responsible for anthraquinone degradation. These results suggest that Sphingomonas and Phenylobacterium species are associated with anthraquinone degradation and that anthracene-degrading organisms may not possess mechanisms to grow on anthraquinone.O xygenated polycyclic aromatic hydrocarbons (oxy-PAHs) such as quinones are cocontaminants in PAH-contaminated soils and sediments (1-3). They are of concern because they have been identified to be toxic and/or genotoxic, either as pure compounds (2, 4-6) or by association with genotoxic fractions of fractionated extracts from contaminated soils and sediments (7-9). In addition, because they are more polar than the parent PAHs, oxyPAHs can exhibit greater mobility within a contaminated environmental system (2, 10). Little is known about the fate of oxyPAHs in contaminated systems, in part because relatively few studies have attempted to identify these compounds and the analytical methods have not yet been standardized (11,12).It is not possible to assess the source(s) or fate of oxy-PAHs in contaminated systems from observation of their presence alone, although it has been suggested that the ratio of an oxy-PAH to the parent PAH can be diagnostic of the source (3, 13). Oxy-PAHs can be present in the same source as the PAHs (2) (such as coal tars), formed in the atmosphere by heterogeneous reactions on particles containing PAHs (14) that can reach soil or sediment by deposition, or produced by chemical or photochemical oxidation of PAHs in situ (2). Oxy-PAHs can also result from microbial transformat...

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Section: Discussionmentioning

confidence: 52%

Identification of Anthraquinone-Degrading Bacteria in Soil Contaminated with Polycyclic Aromatic Hydrocarbons

Zhang

Adrion

et al. 2015

Appl Environ Microbiol

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“…Sequences from the Terrimonas genus also comprised a significant percentage of a bacterial consortium degrading HMW PAHs derived from creosote that had been depleted of LMW-PAHs (Tauler et al 2016). Sequences from the Luteimonas genus (OTU-62) were linked to the co-metabolism of benzo[ a ]pyrene in prior work in our group (Jones et al 2014) and have been identified in a bacterial consortium that degraded pyrene in sediments (Bacosa and Inoue 2015). One sequence with high similarity to the uncharacterized Chitinophagaceae (OTU-36) representative sequence was an excised DGGE band derived from bacteria in heavy-oil-contaminated soil (Lladó et al 2012) and notably, uncharacterized bacteria within the family Chitinophagaceae were also identified as putative HMW-PAH degraders in community analyses of a separate, creosote-contaminated soil (Lladó et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Surfactant-induced bacterial community changes correlated with increased polycyclic aromatic hydrocarbon degradation in contaminated soil

Singleton

Adrion

Aitken

2016

Appl Microbiol Biotechnol

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Bioremediation as a method for removing polycyclic aromatic hydrocarbons (PAHs) from contaminated environments has been criticized for poor removal of potentially carcinogenic but less bioavailable high-molecular-weight (HMW) compounds. As a partial remedy to this constraint, we studied surfactant addition at sub-micellar concentrations to contaminated soil to enhance the biodegradation of PAHs remaining after conventional aerobic bioremediation. We demonstrated increased removal of 4- and 5-ring PAHs using two nonionic surfactants, polyoxyethylene(4)lauryl ether (Brij 30) and polyoxyethylene sorbitol hexaoleate (POESH), and analyzed bacterial community shifts associated with those conditions. Eight groups of abundant bacteria were implicated as potentially being involved in increased HMW PAH removal. A group of unclassified Alphaproteobacteria and members of the Phenylobacterium genus in particular showed significantly increased relative abundance in the two conditions exhibiting increased PAH removal. Other implicated groups included members of the Sediminibacterium, Terrimonas, Acidovorax, and Luteimonas genera, as well as uncharacterized organisms within the families Chitinophagaceae and Bradyrhizobiaceae. Targeted isolation identified a subset of the community likely using the surfactants as a growth substrate but few of the isolates exhibited PAH-degradation capability. Isolates recovered from the Acidovorax and uncharacterized Bradyrhizobiaceae groups suggest the abundance of those groups may have been attributable to growth on surfactants. Understanding the specific bacteria responsible for HMW PAH removal in natural and engineered systems and their response to stimuli such as surfactant amendment may improve bioremediation efficacy during treatment of contaminated environmental media.

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“…For instance, naphthalene and methylnaphthalene inhibited phenanthrene degradation by Pseudomonads [55]. Jones et al [56] also showed that addition of some LMW PAHs inhibited the degradation of spiked benz[a]anthracene in contaminated soils due to a competitive inhibition of PAH metabolism that can occur when compounds are incubated together and more than one substrate is metabolized by the same enzymes.…”

Section: Degradation Selectivity Among Pahsmentioning

confidence: 99%

Bioremediation of PAH-contamined soils: Consequences on formation and degradation of polar-polycyclic aromatic compounds and microbial community abundance

Biache

Ouali

Cébron

et al. 2017

Journal of Hazardous Materials

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A bioslurry batch experiment was carried out over five months on three polycyclic aromatic compound (PAC) contaminated soils to study the PAC (PAH and polar-PAC) behavior during soil incubation and to evaluate the impact of PAC contamination on the abundance of microbial communities and functional PAH-degrading populations. Organic matter characteristics and reactivity, assessed through solvent extractable organic matter and PAC contents, and soil organic matter mineralization were monitored during 5 months. Total bacteria and fungi, and PAH-ring hydroxylating dioxygenase genes were quantified. Results showed that PAHs and polar-PACs were degraded with different degradation dynamics. Differences in degradation rates were observed among the three soils depending on PAH distribution and availability. Overall, low molecular weight compounds were preferentially degraded. Degradation selectivity between isomers and structurally similar compounds was observed which could be used to check the efficiency of bioremediation processes. Bacterial communities were dominant over fungi and were most likely responsible for PAC degradation. Abundance of PAH-degrading bacteria increased during incubations, but their proportion in the bacterial communities tended to decrease. The accumulation of some oxygenated-PACs during the bioslurry experiment underlines the necessity to monitor these compounds during application of remediation treatment on PAH contaminated soils.

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Association of Growth Substrates and Bacterial Genera with Benzo[a]pyrene Mineralization in Contaminated Soil

Cited by 35 publications

References 55 publications

Identification of Anthraquinone-Degrading Bacteria in Soil Contaminated with Polycyclic Aromatic Hydrocarbons

Identification of Anthraquinone-Degrading Bacteria in Soil Contaminated with Polycyclic Aromatic Hydrocarbons

Surfactant-induced bacterial community changes correlated with increased polycyclic aromatic hydrocarbon degradation in contaminated soil

Bioremediation of PAH-contamined soils: Consequences on formation and degradation of polar-polycyclic aromatic compounds and microbial community abundance

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