Association of Gut Microbiota and Biochemical Features in a Chinese Population With Renal Uric Acid Stone

Abstract

Previous studies suggest that patients with nephrolithiasis exhibit dysbiosis in their gut microbiota, but those studies were conducted in calcium oxalate stone patients. We aimed to explore the association of gut microbiota and biochemical features of renal uric acid stone (UAS) patients in a Chinese population and identify the related bacteria that may affect the pathopoiesis of UAS. A case-control study of 117 patients with UAS, 123 patients with gout, and 135 healthy controls were included from January 2014 to October 2020. For each subject, data on demographics, biochemical parameters of blood and urine were analyzed. Fifteen patients with gout, 16 patients with UAS, 17 UAS patients with gout, and 17 healthy subjects were enrolled and provided fecal samples. The characteristics of gut microbiota were explored by using 16S ribosomal RNA (rRNA) gene sequencing and analyzed by using a combination of software mother and R. Hyperuricemia was the main risk factor for the development of gout and UAS. Obesity, dyslipidemia, and aciduria were unique risk factors for UAS patients. The richness, diversity, and relative abundance of dominant bacteria at the phylum and genus levels of gut microbiota in UAS patients were significantly distinct from other subjects. Abundance of Bacteroides and Fusobacterium was significantly positively correlated with the serum uric acid (UA) level of UAS patients. Fusobacteria was involved in the metabolism and degradation of certain short-chain fatty acids, amino acids, and sugars in pathopoiesis of UAS, and inhibited their synthesis pathways. Fusobacteria may be related to the pathogenesis of UAS, and this finding contributes to the personalized treatment of UAS from the perspective of maintaining micro-ecological equilibrium in gut.

Potassium sodium hydrogen citrate intervention on gut microbiota and clinical features in uric acid stone patients

“…Daudon et al ( 2006b ) found that 35.7% of diabetic patients developed UAS compared to only 11.3% among non-diabetic stone patients. Additionally, dyslipidemia like hypertriglyceridemia and low HDL-cholesterolemia were prevalent among 117 UAS patients included in our single-center retrospective study, further supporting our hypothesis that high uric acid level combined with dyslipidemia may contribute to the pathogenesis through mechanisms involving atherosclerosis and inflammation (Cao et al 2022 ).…”

Modulation of gut microbiota and serum metabolome by Apostichopus japonicus derived oligopeptide in high-fructose diet-induced hyperuricemia in mice

“…A study suggested that the significant increase of Muribaculum could result in disorders such as inflammation, dyslipidemia, and glucose [45] . And the increase of Bacteroides has also been proved to be positively correlated with uric acid content [46] . In addition, some studies have also confirmed that the increase of Prevotella and Bacteroides is common in the intestines of hyperuricemic animals [47][48] .…”

Association between salivary microbiota and renal function in renal transplant patients during the perioperative period

“…The relative abundance of Fusobacteria in patients with immunoglobulin A nephropathy or membranous nephropathy exhibited significant elevation when compared with healthy controls (Hu et al, 2020;Zhang et al, 2020;Sugurmar et al, 2021). The microbiota structure showed the same change in type 2 diabetes mellitus, chronic kidney disease, and renal uric acid stone patients (Salguero et al, 2019;Cao et al, 2022). Deltamethrin, as a widely used pyrethroid insecticide, had brought serious problems to the healthy breeding of aquatic animals.…”