Association of heat shock protein 70 gene polymorphisms with acute thermal tolerance, growth, and egg production traits of native chickens in Taiwan

Abstract

Abstract. Heat stress is among the most challenging environmental conditions affecting commercial poultry. It severely affects growth and egg production, particularly in tropical and subtropical regions. This study aimed to examine physiological responses -including triiodothyronine (T 3 ) levels, enzymatic activity of creatine kinase (CK) and lactate dehydrogenase (LDH), respiratory rates, and cloacal temperature -to acute heat stress associated with different genotypes of the HSP70 gene and to evaluate the association of these polymorphisms with growth and egg production. Genotyping was performed by single-strand conformation polymorphism analysis. The polymorphisms identified were A258A, A258G, and G258G. Twenty 12-week old birds were randomly selected from each genotype and exposed to 40 • C ambient temperature for 1 h. Blood samples were collected at 0 and 1 h following heat stress. Respiratory rate and cloacal temperature were measured following 0, 30, and 60 min of exposure. After 1 h, the A258A genotype exhibited lower levels of CK activity and plasma T 3 . Neither respiratory rate nor cloacal temperature displayed a significant association with the genotypes. Body weight gain differed among the genotypes for males (F = 3.268, P = 0.041) and females (F = 14.029, P < 0.001), and the A258A genotype exhibited the greatest weight gain at 0-16 weeks of age for both genders. There were no significant differences among genotypes regarding egg weight at first egg or the number of eggs laid until 40 weeks of age. The A258A genotype displayed higher heat tolerance with no negative effects on growth performance and egg production.

Genetic Diversity of the Structure of HSP70 Gene in Kampung Unggul Balitbangtan (KUB), Walik, and Kate Walik Chickens

“…Three of the detected alleles (hsp70-1, hsp70-2, and hsp70-3) were associated with the nature of resistance to heat stress, and therefore, it's potential to be used as a candidate gene for heat stress (Mazzi et al, 2003). The results of this study have encouraged additional research in the other chickens: Indonesian local chicken (Tamzil et al, 2013a), Taiwanese local chicken (Liang et al, 2016), and Thailand local chicken (Duangjinda et al, 2017).…”

“…Based on the associations observed between chicken breeds and HSP70 gene genotypes and heat stress, native chickens appear to have the best heat resistance, while the most highly heat-tolerant genotype is AD (Tamzil et al, 2013b). Liang et al (2016) have shown the association between HSP70 gene polymorphisms detected by PCR-SSCP method with the acute temperature tolerance, growth, and egg production in Taiwanese local chickens. Only AA genotype (g.258A>G) shows a high tolerance level to acute temperatures (at 40 °C for 1 hour), without affecting the growth performance and egg production (Liang et al, 2016).…”

“…Liang et al (2016) have shown the association between HSP70 gene polymorphisms detected by PCR-SSCP method with the acute temperature tolerance, growth, and egg production in Taiwanese local chickens. Only AA genotype (g.258A>G) shows a high tolerance level to acute temperatures (at 40 °C for 1 hour), without affecting the growth performance and egg production (Liang et al, 2016). Thailand local and broiler chickens produce C and M loci, based on PCR-RFLP analysis with the restriction enzymes of CfrI and MmeI (New England Biolabs, Ipswich, MA) in coding region of the HSP70 gene, but only C1C1 and C1C2 genotypes that can be used as heat stress markers (Duangjinda et al, 2017).…”

“…The silent mutations occurred in the coding region of the chicken HSP70 gene have been used as molecular markers for determining heat resistance in chickens (Mazzi et al, 2003). Polymorphisms in the chicken HSP70 gene are detected by the PCR single-strand conformation polymorphism (PCR-SSCP) method (Tamzil et al, 2013a;Liang et al, 2016) and PCR restriction fragment length polymorphism (PCR-RFLP) analysis (Duangjinda et al, 2017). Research on the HSP70 gene using the PCR-SSCP method has succeeded in identifying seven genotypes in Kampung Lombok chickens (AA, AB, AC, CC, AD, DD, and BC), six genotypes in Arabian chickens (AA, AB, AC, CC, AD, and BC), but only one genotype (DD) in commercial laying chickens (Tamzil et al, 2013a).…”

HSP70 And HSP90β Genes Polymorphism And Its Association With Thermotolerance In Fayoumi And Leghorn Chicken Breeds

“…Chen et al, (2016) suggested that, the GG genotype of HSP70 gene 5'-flanking region is advantageous for the prevention of thermal stress. Genotyping and allelic frequency variation of HSP70 gene by Liang et al, (2016) indicated that AA genotype has greater heat tolerance after acute heat stress in the indigenous Taiwanese chickens. Results of HSP70 gene genotyping by Duangjinda et al, (2017) using CfrI restriction enzyme also are indicated that C2C2 chickens were less tolerant to heat stress compared to other genotypes.…”