Association of homocysteine thiolactonase activity and PON1 polymorphisms with the severity of acute coronary syndrome

Koubaa, Nadia; Nakbi, Amel; Hammami, S.; Attia, N.; Mehri, Sounira; Hamda, Khaldoun Ben; Farhat, M. Ben; Miled, Abdelhédi; Hammami, Mohamed

doi:10.1016/j.clinbiochem.2009.02.017

Cited by 29 publications

(22 citation statements)

References 32 publications

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“…Regarding the R allele an increasing trend was present in QR and RR genotypes. Thus, the effect of PON1 Q192R polymorphism on serum PON1 activity was similar to that previously reported 33,34,36) . There was no significant difference in PON1 Q192R polymorphism frequency between control and patient groups.…”

Section: Discussionsupporting

confidence: 74%

“…Using paraoxon and HTL as substrates, we have recently demonstrated that PON1 192R hydrolyzed both substrates with higher affinity 36) . Moreover, in two independent clinical studies, higher serum HTase activity was shown to be associated with 192R allele 33,34) . In the present study, using either paraoxon or HTL as a substrate, serum PON1 activity was found to be significantly lower in the patient group and the lowest activity was observed in individuals with QQ genotype.…”

Section: Discussionmentioning

confidence: 99%

“…It is now well established that PON1 possesses HTase activity that degrades an endogenous substrate, HTL, involved in the oxidative modification of proteins; thus, we evaluated HTase activity in addition to PONase in serum samples. In the literature, two studies have evaluated the relationship among the PON1 genotype, HTase activity and CAD risk 33,34) . These studies were performed with a limited number of subjects and the control group consisted of asymptomatic individuals.…”

Section: Discussionmentioning

confidence: 99%

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Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Bayrak

Tokgözoğlu

et al. 2012

JAT

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Aim: Paraoxonase-1 (PON1) is an antioxidant enzyme located in high density lipoprotein (HDL). PON1 was defined as a protective factor against atherosclerosis. The aim of this study was to investigate the possible relationship between serum paraoxonase (PONase), homocysteine thiolactonase (HTase) activities and PON1 Q192R polymorphism, and the extent and severity of atherosclerosis. Methods: Blood specimens were collected from 142 individuals who had no coronary artery lesions angiographically (control group) and 128 individuals who had angiographically documented coronary artery disease of several degrees (patient group). The extent and severity of arterial lesions were evaluated by the Gensini scoring system. PONase and HTase activities were measured in serum using a spectrophotometric method. PON1 Q192R polymorphism was evaluated using PCR-RFLP after DNA isolation from blood. Results: Serum PONase and HTase activities were significantly lower in the patient group than in healthy controls (135.7 56.0 U/mL vs 153.8 62.0 U/mL, p 0.05; 36.0 6.1 U/mL vs 43.0 4.04 U/mL, p 0.01; respectively). In the patient group, there was a negative correlation between PONase, HTase activities and the Gensini score (r 0.168, p 0.039; r 0.164, p 0.006, respectively). In both groups, there was no significant difference in the distribution of PON1 Q192R polymorphism. In the patient group, the distribution of Gensini scores according to genotypes was not significant. Conclusion: It has been concluded that serum PONase and HTase activities might be a more relevant marker than PON1 genotype in evaluating the extent and severity of atherosclerosis.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Bayrak

Tokgözoğlu

et al. 2012

JAT

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“…Previous studies have demonstrated decreased PON1 lactonase activity in HD patients compared with healthy subjects [23][24][25], moreover; HD patients were found to be susceptible to lipid peroxidation, protein homocysteinylation and, hence, atherosclerosis [23,24]. PON1 lactonase activity was significantly lowered in patients with coronary artery disease (CAD) than in controls [26,27]. Serum levels of total Hcy and oxidized LDL increased significantly in CAD patients and were associated with a significant decrease in both the levels of HDL cholesterol and the PON1 lactonase activity.…”

mentioning

confidence: 90%

“…Serum levels of total Hcy and oxidized LDL increased significantly in CAD patients and were associated with a significant decrease in both the levels of HDL cholesterol and the PON1 lactonase activity. In CAD patients, the PON1 lactonase activity was negatively associated with total Hcy and high-sensitivity C-reactive protein (CRP) levels [26]. The possible association between PON1 lactonase activity and occurrence of AV fistula thrombosis in HD patients remains unexplored.…”

mentioning

confidence: 99%

Circulating Fibroblast Growth Factor-23 Level and Paraoxonase-1 Lactonase Activity in Chronic Hemodialysis Patients: Their Impact on the Incidence of Native AV Fistula Thrombosis

Zohny¹,

El-Fattah²,

Khan³

2016

CIM

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Purpose: Thrombosis of native arteriovenous (AV) fistula is an important cause of complications in hemodialysis (HD) patients. The purpose of this study was to investigate the usefulness of measuring circulating fibroblast growth factor-23 (FGF-23) level and paraoxonase-1 (PON1) lactonase activity as potential predictors of native AV fistula thrombosis in chronic HD patients.Methods: This study included 83 HD patients (48 with thrombosed and 35 with non-thrombosed native AV fistulas) and 38 healthy volunteers. Serum FGF-23 level was measured using the ELISA technique, while serum PON1 lactonase activity was measured spectrophotometrically using gamma-thiobutyrolactone as a substrate.Results: FGF-23 was significantly increased while PON1 lactonase was markedly decreased in both thrombosed and non-thrombosed HD patients compared with controls (P < 0.001). FGF-23 was elevated whereas PON1 lactonase was decreased in HD patients with thrombosed native AV fistulas compared with HD patients with non-thrombosed native AV fistulas (P = 0.001 and 0.002, respectively). A significant negative correlation was found between FGF-23 and PON1 lactonase in HD patients with thrombosed native AV fistulas (r = −0.342, P = 0.017).Conclusions: This study shows a potential value of FGF-23 and PON1 lactonase as predictors of native AV fistula thrombosis in HD patients.

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Altered Composition

2011

High‐Density Lipoproteins

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Association of homocysteine thiolactonase activity and PON1 polymorphisms with the severity of acute coronary syndrome

Cited by 29 publications

References 32 publications

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Circulating Fibroblast Growth Factor-23 Level and Paraoxonase-1 Lactonase Activity in Chronic Hemodialysis Patients: Their Impact on the Incidence of Native AV Fistula Thrombosis

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