Association of HSL gene E1-c.276C>T and E8-c.51C>T mutation with economical traits of Chinese Simmental cattle

Abstract

Hormone-sensitive lipase (HSL) is responsible for the decomposition of triglycerides in adipose tissue to release free fatty acids, and it is a key rate-limiting enzyme in the regulation of adipose tissue deposition and decomposition. The objective of this study was to evaluate the association between novel SNPs in the coding region of bovine HSL gene and carcass and meat quality traits of Chinese Simmental-cross steers. Two novel SNPs were genotyped and the 47 traits of carcass and meat quality traits were measured in the population studied. Statistical analysis revealed that the SNPs of HSL gene were associated with the carcass and meat quality traits. The individuals with TT genotypes of E1-276C>T showed significant higher dressing percentage, net meat rate, hind legs circumference, fat coverage rate, mesenteric fat and kidney fat (p < 0.05). E8-51C>T (P17S) also showed a significant association with the pH of beef and fatty acids content in Chinese Simmental cattle (p < 0.01). Our findings indicated that polymorphisms in HSL might be one of important genetic factors that influence carcass yield and meat quality in beef cattle, and it may be a useful marker for meat quality traits in future marker-assisted selection programs in beef cattle breeding and production.

Computational Analysis of the Sequences of LIPE Gene of Selected Ruminants and Non-ruminants

“…1). The present findings showed that the LIPE gene of pig, cattle, water buffalo, camel, goat and sheep is polymorphic in nature and earlier studies have associated polymorphism of the LIPE gene with superior milk yield and milk fat contents, carcass characteristics including muscle thin, fat thickness of back, intra-muscular fat in different species etc [8,9,10,19]. More so, the amino acid substitution revealed both neutral and deleterious variants of LIPE gene in all the six studied species.…”

“…The amino acid substitution that returned neutral indicates that the substitution did not have any damaging effect on the structure and function of the protein while those deleterious amino acid substitution predictions were harmful and could cause an alteration or impairment of protein functions which may lead to impair energy balance, low levels of circulating fatty acid, adipocyte hypertrophy, lipid-filled macrophages and an increase in basal lipolysis particularly in lactating animals [20,21,22]. This is because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland [8]. The phylogenetic tree obtained based on the nucleotide sequences of the studied species suggests that goat was more related with water buffalo.…”

“…These fatty acids can be subsequently transferred to other body compartments to be oxidized for other biochemical reactions particularly in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. Single Nucleotide Polymorphism in LIPE have been associated with milk yield and milk fat contents of goats [8], carcass and meat quality traits in steers [9], superior carcass and meat quality traits in pigs [10] and intra-muscular fat and fatty acid composition of bovine meat [8]. Since LIPE has direct effects in lipolysis, structural changes on the enzyme are expected to modify the fatty acid composition of tissues which may also affect many other economic traits particularly those that require free fatty acids as metabolic precursors [11,12].…”

Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese black (Wagyu) and Chinese Red Steppes cattle

“…Many of the differentially methylated genes identified in the present study between Wagyu and Chinese Red Steppes are also found to be differentially methylated in genes related to lipid metabolism and fatty acid metabolism, such as fatty acid synthase ( FASN ) which is shown to be related to fatty acid composition in a genome-wide association study of Japanese black cattle [43], and carnitine palmitoyltransferase 1A ( CPT1A ), fatty acid desaturase 1 ( FADS1 ) and acyl-CoA synthetase long-chain family member 1 ( ACSL1 ) genes which are well-known genes in the pathways of fatty acid metabolism and fatty acid degradation. In addition, other genes known to affect meat quality traits of cattle are also related to the lipid metabolism and fatty acid metabolism ( insulin like growth factor 2 ( IGF2 ), leptin ( OB ), lipase hormone sensitive ( HSL ), diacylglycerol O-acyltransferase 1 ( DGAT1 ) and fatty acid binding protein 3 ( FABP3 )) [44–49]. We believed that the methylation of these genes might partially contribute to the significant variance in meat quality traits between Japanese black cattle and Chinese Red Steppes.…”

Characterization of the bovine gene LIPE and possible influence on fatty acid composition of meat

“…, 2005 ). Recently, two SNPs have been successfully associated with carcass and meat quality traits in Chinese Simmental-cross steers ( Fang et al . , 2013 ).…”