Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population

Abstract

GG ‫پلي‬ ‫مورفيسم‬ HSP70-2 1267A/G ‫ترتيب‬ ‫(به‬ ‫داشت‬ ‫کنترل‬ ‫گروه‬ ‫به‬ ‫نسبت‬ ‫بيشتري‬ ‫فراواني‬ ‫بيمار‬ ‫گروه‬ ‫در‬ AbstractBackground: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients. Material and Methods:This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1). Results:The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005). Conclusion:The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results.

The Contribution of Fluoride to the Pathogenesis of Eye Diseases: Molecular Mechanisms and Implications for Public Health

“…Of note, Hsp 40 has also been found to protect the lens from stress induced denaturation [ 195 ], while Hsp27 expression is thought to play a role in age-related macular degeneration [ 196 ] and cataractogenesis [ 197 ]. In addition, variants of Hsp70 have been found to serve as genetic susceptibility factors for susceptibility to cataract and glaucoma in humans [ 198 , 199 , 200 ]. Moreover, c-Myc binding proteins play an essential role in promoting lens growth and differentiation [ 201 , 202 ] and inactivation of c-myc results in severe eye and lens growth impairment and anterior chamber malformation [ 202 , 203 ].…”