Association of Human Papilloma Virus and Epstein-Barr Virus with Ovarian Cancer in Shiraz, Southwestern Iran

Abstract

Scan to discover online Background & Objective: Ovarian cancer is one of the most common cancers amongst women. The association of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) with ovarian cancer is inconclusive; therefore, the aims of this study were to evaluate the frequency of HPV and EBV in malignant, borderline, benign and normal ovarian tissues. Methods: In this case-control study, 205 Paraffin-embedded ovarian tissue specimens including 68 malignant, 27 borderline, 65 benign, and 45 normal tissues were included from December 2014 to January 2018 and subjected to DNA extraction. The β-globin gene was amplified using PCR to confirm the quality of the extracted DNA. The genomes of HPV (genotypes 16 and 18) and EBV were identified, using specific primers by PCR. Results: The mean age of participants was 43.42 ± 15.4 years. The frequency of HPV was statistically significant between malignant versus benign (P=0.02) and control groups (P=0.002), but not with borderline tumor group (P=0.78). Amongst HPV infected samples, 1 (4.5%) and 14 (63.6%) samples were infected with types 16 and 18, respectively. Also 4 (18.2 %) samples were infected with both genotypes. Eleven samples including 7(10.3%) malignant, 1 (3.7%) borderline, 3 (4.6%) benign and none (0%) of normal control groups were infected with EBV, which was statistically different between malignant and the normal control group (P=0.03). Conclusion: The results of our study showed the possible role of high risk HPVs as well as EBV in pathogenesis of ovarian cancer, and further studies are recommended to confirm these findings.

Development and validation of ferroptosis-related lncRNA features to improve prognosis prediction in ovarian cancer

“…Epstein-Barr virus (EBV) is a double-stranded DNA virus [39]. In two different studies, the EBV positivity rate has been reported to be lower in the normal group than in OC patient groups [40,41]. It has moreover been reported that elevated EBV antibody titers may lead to increased risk of OC [42].…”

An Unusual Presentation of Giant Fibroepithelial Polyp of the Vagina

“…Treatment of FEPV involves simple local excision, and its recurrence is uncommon in patients (17,18). In pregnant women, surgery can be performed after pregnancy when vaginal vascularity has returned to normal (19).…”

Association of Epstein–Barr Virus and Cytomegalovirus Infections with Esophageal Carcinoma

“…Ten sections (10 µm) of the paraffin-embedded block were cut and undergo de-paraffinization as previously described (5). DNA was extracted using tissue DNA extraction Minikit (Yektatajhiz, Tehran, Iran) according to the manufacturer's instruction.…”

“…Viral infections including HPV, Epstein-Barr virus (EBV), hepatitis B and C viruses, human T-lymphotropic virus-1, and polyomaviruses are considered as the major risk factor for about 20% of all cancers (4)(5)(6). Thus, some studies proposed a possible link between viruses such as EBV, HPV, and cytomegalovirus (CMV) with esophageal carcinoma (7)(8)(9).…”