1984
DOI: 10.1128/jb.158.1.373-375.1984
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Association of hydrogen metabolism with unitrophic or mixotrophic growth of Methanosarcina barkeri on carbon monoxide

Abstract: Methanosarcina barkeri was adapted to grow on carbon monoxide by sequential transfer of the culture in medium that contained CO (100% of culture headspace). These experiments document the ability of the organism to grow slowly (65-h doubling time) and to produce methane and CO2 either on CO as the sole carbon and energy source or by the simultaneous consumption of methanol and CO. During growth on CO as carbon and energy source, net hydrogen formation occurred when the CO partial pressure in the culture headsp… Show more

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Cited by 113 publications
(55 citation statements)
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“…The absence of direct methanogenic CO conversion, as found for Nedalco sludge at 30 ‡C, might be explained by a complete inactivation of methanogens due to the high CO concentration in the gas phase [2]. Only M. barkeri has so far been reported to grow at 100% CO in the gas phase after long-term adaptation to slowly increasing CO concentrations [13]. Disrupting the granular structure of the methane-producing sludges resulted in a strongly increased exposure of the active biomass to CO and led to a strong inhibition of methane formation.…”
Section: Discussionmentioning
confidence: 90%
See 1 more Smart Citation
“…The absence of direct methanogenic CO conversion, as found for Nedalco sludge at 30 ‡C, might be explained by a complete inactivation of methanogens due to the high CO concentration in the gas phase [2]. Only M. barkeri has so far been reported to grow at 100% CO in the gas phase after long-term adaptation to slowly increasing CO concentrations [13]. Disrupting the granular structure of the methane-producing sludges resulted in a strongly increased exposure of the active biomass to CO and led to a strong inhibition of methane formation.…”
Section: Discussionmentioning
confidence: 90%
“…Methanothermobacter thermoautotrophicus [12], and acetotrophic, e.g. Methanosarcina barkeri [13], methanogenesis.…”
Section: Introductionmentioning
confidence: 99%
“…Methanogenic, acetogenic, and sulfate-reducing bacteria are known to oxidize CO via a carbon monoxide dehydrogenase [6]. Several methanogenic strains consume CO during growth on H P and CO P , but growth on CO is very limited [17,24]. Desulfovibrio vulgaris utilizes CO for energy and sulfate reduction, but concentrations s 4.5% in the headspace are inhibitory [14].…”
Section: Discussionmentioning
confidence: 99%
“…Vancomycin and erythromycin are bacterial inhibitors and the fact that no CO conversion was observed in their presence indicates that CO was not directly metabolized by carboxydotrophic methanogens. Carbon monoxide is inhibitory for most methanogens, and only a few species can actually grow on this substrate: Methanothermobacter thermoautotrophicus (Daniels et al, 1977), Methanothermobacter marburguensis (Diender et al, 2016), Methanosarcina barkeri (O'Brien et al, 1984;Bott et al, 1986) and Methanosarcina acetivorans (Rother and Metcalf, 2004). Both thermophilic Methanothermobacter species grow significantly slower on CO than on H 2 /CO 2 (Daniels et al, 1977;Diender et al, 2016).…”
Section: Co To Methane Conversion By Mobb Enrichments Is Dependent Onmentioning
confidence: 99%