Association ofCILP2andACEGene Polymorphisms with Cardiovascular Risk Factors in Slovak Midlife Women

Abstract

The aim of this study is to assess the association of two polymorphisms, the cartilage intermediate layer protein 2 (CILP2) G/T and angiotensin converting enzyme (ACE) I/D, with blood pressure and anthropometrical and biochemical parameters related to the development of cardiovascular disease. The entire study sample comprised 341 women ranging in age from 39 to 65 years. The CILP2 genotypes were determined by PCR-RFLP and the ACE genotypes by PCR. The Bonferroni pairwise comparisons showed the effect of the CILP2 genotype on high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein B (apoB), apoB-to-apoA1 ratio, the total cholesterol (TC)-to-HDL-C ratio, non-HDL-C, and the LDL-C-to-HDL-C ratio (P < 0.05). Here, higher mean levels of HDL-C and lower mean levels of the remaining above mentioned lipid parameters were registered in the GT/TT genotype carriers than in GG carriers. Statistically significant association was identified between the ACE genotype and the following parameters: TC, LDL-C, and non-HDL-C (P < 0.05). The II genotype can lower serum level of TC (B = 0.40), LDL-C (B = 0.37), and non-HDL-C levels. The results of this study suggest that the minor T allele of CILP2 gene and I allele of ACE gene have a protective effect against elevated serum lipid and lipoprotein levels.

CILP2 overexpression correlates with tumor progression and poor prognosis in patients with colorectal cancer in the study of The Cancer Genome Atlas (TCGA) and our cohort analysis

“…Rašlová K, et al's report on the association between CILP2 polymorphism and FER(HDL) supports its role in lipid metabolism [22] . While in other reports, the relationship between CILP2 polymorphism and lipid metabolism was not yet discovered [23] , nor in the Japanese population [24] or Slovak Midlife women [25] . However, Lenka et al indicated that the minor T allele in the CILP2 gene was associated with lower LDL-C, apoB, and atherogenic indices and higher HDL-C levels [25] .…”

“…While in other reports, the relationship between CILP2 polymorphism and lipid metabolism was not yet discovered [23] , nor in the Japanese population [24] or Slovak Midlife women [25] . However, Lenka et al indicated that the minor T allele in the CILP2 gene was associated with lower LDL-C, apoB, and atherogenic indices and higher HDL-C levels [25] . This result was by the study in the Singaporean population ranging from 40 to 80 years of age [26] .…”

CILP2 overexpression predicts poor prognosis in colorectal cancer

“…According to Kathiresan et al [8] , in Caucasian individuals analyzed, rs16996148 variant of CILP2 gene had a reducing role in triglyceride and LDL-C level. While in other reports, the relationship between CILP2 polymorphism and lipid metabolism was not yet discovered [18] , nor in Japanese population [19] or in Slovak Midlife women [20] . However, Lenka et al indicated that the minor T allele in CILP2 gene was associated with lower LDL-C, apoB, and atherogenic indices and higher HDL-C levels [20] .…”

“…While in other reports, the relationship between CILP2 polymorphism and lipid metabolism was not yet discovered [18] , nor in Japanese population [19] or in Slovak Midlife women [20] . However, Lenka et al indicated that the minor T allele in CILP2 gene was associated with lower LDL-C, apoB, and atherogenic indices and higher HDL-C levels [20] . This result was in accordance with the study in Singaporean population ranging from 40 to 80 years of age [21] .…”

CILP2 overexpression predicts poor prognosis in colorectal cancer

“…According to Kathiresan et al [8] , in Caucasian individuals analyzed, rs16996148 variant of CILP2 gene had a reducing role in triglyceride and LDL-C level. While in other reports, the relationship between CILP2 polymorphism and lipid metabolism was not yet discovered [20] , nor in Japanese population [21] or in Slovak Midlife women [22] . However, Lenka et al indicated that the minor T allele in CILP2 gene was associated with lower LDL-C, apoB, and atherogenic indices and higher HDL-C levels [22] .…”