Association of Pepino mosaic virus with Tomato Collapse

Abstract

Since 1998 collapse of tomato plants has been producing significant losses in protected tomato crops along the Spanish Mediterranean coast. Affected plants show sudden and progressive wilt followed by total collapse and death. Analysis of the distribution and accumulation of Pepino mosaic virus (PepMV) in collapsed and non-collapsed plants and the presence of the virus in all analysed collapsed plants suggests a relationship of this Potexvirus with tomato collapse. The fact that collapse does not occur on PepMV-free farms reinforces this hypothesis. PepMV-infected plants showed a necrotic area in the vascular system, mainly in the basal area of the stem. This necrosis was more pronounced in collapsed plants and in the areas with higher viral concentration. Our hypothesis is that tomato collapse would be associated with necrosis of the vascular system caused by PepMV accumulation. Materials and Methods Analysis and incidence of collapsed plantsPlant analyses were carried out on the commercial hybrids cultivated in the south-eastern area of Spain, mainly Rambo F1, Durinta F1, Daniela F1 and Gabriela F1, which are resistant to some of the most www.blackwell-synergy.com

El virus del mosaico del pepino dulce (Pepino mosaic virus) afectando al cultivo del tomate (Solanum lycopersicum): Caracterización y Epidemiología

“…Por otro lado, la aparición del síndrome del "colapso" del tomate ha producido importantísimas pérdidas en los cultivos españoles, principalmente en la zona de Murcia. En esta Comunidad en la campaña de 1999/2000, más del 90% de los invernaderos se vieron afectados por el colapso con una incidencia variable de entre el 15-90% de plantas afectadas dentro del mismo invernadero (Soler-Aleixandre et al, 2005b).…”

“…Previous reports (Soler-Aleixandre et al, 2005) only detected the presence of collapse located in the Mazarrón area (the Province of Murcia). The present study shows that wilt symptoms are widely distributed throughout an area, which includes the Murcia Region, Almería, Alicante, Granada, and the Canary Islands.…”

“…The research groups detected both organisms in 100% of the root samples from the wilted plants. Previous works (Soler-Aleixandre et al, 2005) relate the collapse disorder with necrosis observed in the vascular system, attributed to PepMV accumulation.…”

“…El síndrome de "Colapso" se ha intentado relacionar con una mayor acumulación del PepMV en la parte basal del tallo que provocaría la necrosis del sistema vascular y en consecuencia el marchitamiento súbito en la parte alta de la planta (Soler-Aleixandre et al, 2005b). Al no lograr reproducir la enfermedad bajo condiciones controladas, los autores concluyen que el virus seria un agente necesario, aunque no suficiente, para el desarrollo de la enfermedad, y dejando en el aire la posibilidad de que tambien pudiera estar implicada alguna especie fúngica presente en las raíces (Verticillium, Olpidium), hipótesis que no corroboran en el estudio.…”

Epidemiología y variabilidad patogénica del virus del mosaico del pepino dulce (Pepino mosaic virus). Nuevas enfermedades asociadas a su presencia (torrao o cribado)

“…however collapse was not reproduced in that study so Koch´s third postulate was not fulfilled (Soler-Aleixandre et al, 2005). (Campbell, 1996).…”