Association of IL-6 C-572G Gene Polymorphism with Anti-E Production

Abstract

<b>Background: </b>Interleukin 6 (IL-6) is involved in regulation of immunoglobulin production. The aim of this study was to investigate the association between IL-6 single nucleotide polymorphisms (SNPs) in the IL-6 promoter and anti-E in red blood cell (RBC) transfusion recipients. <b>Methods:</b> 50 healthy subjects, 54 patients with RBC alloantibody anti-E (responders), and 45 patients without alloantibody (non-responders) were recruited. All patients were E antigen-negative. <b>Results: </b>All healthy subjects and patients had GG at -174 position of <i>IL-6</i> gene. In our healthy subjects, the frequency of the -572 CC genotype was 58%, that of the -572 CG genotype 38%, and that of the -572 GG genotype 4%. The frequency of G allele of -572 SNP in responders was significantly higher than that in non-responders, (31.5 vs. 16.7%; p = 0.020). The frequency of -572 G-positive genotypes (CG and GG) in responders was also significantly higher than that in non-responders, (55.6 vs. 31.1%; p = 0.016). The relative risk of RBC alloimmunization for patients with the -572 G-positive genotype was significantly higher than that of patients with the -572 CC genotype, (1.771 vs. 0.640; p<i> = </i>0.016). <b>Conclusion:</b><i>IL-6</i> C-572G gene polymorphism is significantly associated with anti-E production, with the allele G as a risk allele.

HLA‐DRB1 and cytokine polymorphisms in Brazilian patients with myelodysplastic syndromes and its association with red blood cell alloimmunization

“…31 Different studies have found positive association of other cytokine polymorphisms and RBC alloimmunization in different populations. For example, IL6 572GG positive genotype was significantly associated with a high risk for anti-E alloimmunization 32 while TNFA 308A and IL1B 511T were associated with susceptibility to alloantibody in patients with SCD. 9 Although, these data corroborate the role of cytokine polymorphisms in RBC alloimmunization, there is few discussion of this topic in MDS patients.…”

Association of the interleukine-6 polymorphism with catheter-induced coronary artery spasm in Egyptians

“…Genomic DNA was extracted from whole blood using the QIAamp Blood Kit (Qiagen GmbH, Hilden, Germany). The subjects were genotyped to the −572 C>G (rs 1800796) polymorphism of the IL-6 gene by PCR-restriction fragment length polymorphism as described previously [7].…”