Association of inducible nitric oxide synthase with asthma severity, total serum immunoglobulin E and blood eosinophil levels

Batra, Jyotsna; Singh, Tejbir; Mabalirajan, Ulanganathan; Sinha, Aditi; Prasad, Rajendra; Ghosh, Balaram

doi:10.1136/thx.2005.057935

Cited by 13 publications

(20 citation statements)

References 28 publications

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“…Most of the studies involved a putative functional repeat polymorphism of the promoter region, (CCTTT) n , that was associated with atopic status in Japanese adults,17 but not in Chinese children,18 Czech adults16 or in Indian children and adults 15. In the present study, we found no evidence for an effect of this repeat polymorphism on asthma or lung function growth (data not shown).…”

Section: Discussioncontrasting

confidence: 63%

Role of inducible nitric oxide synthase in asthma risk and lung function growth during adolescence

Islam

Breton²,

Salam³

et al. 2009

Thorax

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Background Inducible nitric oxide (NO) synthase (iNOS, encoded by NOS2A) produces NO in response to environmental stimuli, which can result in nitrosative stress. Because nitrosative stress affects respiratory health, it was hypothesised that variants in NOS2A are associated with asthma incidence and lung function growth during adolescence. Methods In this prospective study, spirometric testing was performed at school and a presence or absence of asthma was ascertained annually by questionnaire among children participating in the Southern California Children's Health Study. 24 single nucleotide polymorphisms (SNPs) of the NOS2A region (with seven promoter SNPs in one haplotype block), spanning 20 kb upstream and 10 kb downstream were genotyped. Association between the NOS2A region and asthma or lung function growth was tested using genetic blockspecific principal component and haplotype analyses. This study was restricted to children with Latino and Caucasian ancestry for analyses of both asthma (n¼1596) and lung function growth (n¼2108). Result A pair of "yineyang" haplotypes in the promoter region showed strong association with new-onset asthma and lung function growth. The "yin" haplotype (h0111101) was associated with 44% increased asthma risk (p¼0.003) and reduced forced expiratory volume in 1 s (FEV 1 ) growth from 10 to 18 years of age (À29.46 ml, p¼0.07), whereas the "yang"(h1000010) haplotype was associated with 23% reduced asthma risk (p¼0.13) and better FEV 1 growth (43.84 ml, p¼0.01). Furthermore, the increased asthma risk associated with h0111101 was restricted to children with the GSTM1 "null" genotype (interaction p¼0.002, HR 1.89, 95% CI 1.34 to 2.60). Conclusion Common haplotypes in the NOS2A promoter are associated with new-onset asthma and lung function growth. These effects are stronger in adolescents with the GSTM1 "null" genotype.

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Section: Discussioncontrasting

confidence: 63%

Role of inducible nitric oxide synthase in asthma risk and lung function growth during adolescence

Islam

Breton²,

Salam³

et al. 2009

Thorax

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“…The presence of iNOS transcripts and protein in human eosinophils and an eosinophilic leukemia cell line, Eol-3, has been documented (del Pozo et al 1997). Polymorphisms in the iNOS gene have also been associated with severity of asthma, serum NO levels, and eosinophilia suggesting correlation between NO and the pathogenesis of asthma (Batra et al 2007). Endothelial NOS (eNOS), a constitutive NOS, was not found in human eosinophils (Ferreira et al 2002), even though polymorphism in eNOS is associated with asthma (Lee et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural immunogold localization of nitric oxide synthase isoforms in rat and human eosinophils

et al. 2010

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The involvement of nitric oxide (NO) as both pro and anti-inflammatory agent in allergic, airway inflammatory, and asthmatic diseases and the active participation of eosinophils in such ailments have been previously suggested. NO modulates eosinophil number, migration and their survival. The microenvironment of NO synthase (NOS) in subcellular organelles determines its rate and efficiency of catalysis, which in turn influences NO generation at distinct intracellular locales. The present study was undertaken to assess the intracellular distribution of NOS isoforms by transmission electron microscopy followed by morphometric analysis in human and rat eosinophils. Rat eosinophils were explored in parallel, and since they are widely used as model systems to mimic human diseases, a comparative study on NOS localization patterns might provide useful information in deciphering NO role in diverse aspects of eosinophil-related inflammatory ailments. The results demonstrated predominance of neuronal NOS (nNOS) in the eosinophilic granules and even distribution of inducible NOS (iNOS) and nNOS in the cytoplasm and nucleus of human eosinophils. In rat eosinophils, however, iNOS was mainly localized in the eosinophilic granules and nucleus, while nNOS was distributed evenly in cytoplasm and nucleus. Distribution of endothelial NOS (eNOS) in eosinophils was scanty. Differences in NOS isoforms and their localization in human and rat cells might have implications in differential mode of catalysis and functional contribution to eosinophil physiology and pathology, warranting detailed investigations. The present study highlights species-specific differences in the relative abundance and distribution pattern of NOS isoforms in rat and human eosinophils, which should be considered cautiously in interpreting the rat data to humans.

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“…Tan et al (2009) showed that inclusion of 0.4-0.8% arginine in diets for early-weaned pigs enhances the humoral immune response by modulating serum Ig level. Batra et al (2007) reported an association between inducible nitric oxide synthase (iNOS) and serum total Ig. It has been suggested that NO produced by iNOS may act on Th2 cells to upregulate their production of interleukin 4 (Duguet et al, 2001;Xiong et al, 1999) which increases Ig E expression (Frieri, 2005).…”

Section: Discussionmentioning

confidence: 99%

Dietary arginine requirement of juvenile red sea bream Pagrus major

Rahimnejad

Lee

2014

Aquaculture

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Association of inducible nitric oxide synthase with asthma severity, total serum immunoglobulin E and blood eosinophil levels

Cited by 13 publications

References 28 publications

Role of inducible nitric oxide synthase in asthma risk and lung function growth during adolescence

Role of inducible nitric oxide synthase in asthma risk and lung function growth during adolescence

Ultrastructural immunogold localization of nitric oxide synthase isoforms in rat and human eosinophils

Dietary arginine requirement of juvenile red sea bream Pagrus major

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