Association of Interleukin 13 rs20541 Gene Polymorphism and Serum Periostin with Asthma and Allergic Conjunctivitis Among Egyptian Patients

Abstract

Background Single nucleotide polymorphisms (SNPs) in the interleukin 13 (IL13) gene are associated with vulnerability to allergic diseases, such as asthma and allergic conjunctivitis (AC). Periostin, as an IL13-induced protein, has emerged as a novel biomarker in several allergic diseases. Data among Egyptian patients are still scarce. Aim To find out the association of IL13 rs20541 gene polymorphism and serum levels of periostin with asthma and AC among Egyptian patients. Patients and Methods Eighty-one Egyptian allergic patients with asthma, AC, and both asthma and AC (27 each), were enrolled in this case–control study. Twenty-seven age and gender-matched healthy volunteers served as controls. All participants were tested for IL13 rs20541 SNP by real-time polymerase chain reaction, TaqMan method. Serum levels of periostin and IL13 were assessed by ELISA. Results Compared to healthy subjects, asthmatic patients had a higher frequency of the homozygous adenine/adenine (AA) genotype at IL13 rs20541 SNP (14.8% vs 3.7%) and a lower frequency of the guanosine/guanosine (GG) genotype (51.9% vs 55.6%), while AC patients had higher GG genotype (70.4% vs 55.6%) with no AA genotype detected, yet no significant difference was noticed (p = 7.053). A significantly higher serum periostin in asthmatic patients compared to controls was found (p = 0.005). Higher levels of serum periostin, although nonsignificant, were recorded in AC patients compared to controls (22.88 ± 10.01ng/mL and 17.51 ± 3.17ng/mL, respectively). Periostin was significantly higher in patients with IL13 AA and GA genotypes compared to those with GG genotype (p = 0.016). A significant positive correlation between serum periostin and serum IL13 among allergic patients was recorded (r = 0.352, p < 0.001). Conclusion Among Egyptian patients, serum level of periostin is significantly associated with asthma and positively correlates with IL13 level supporting its utility as a diagnostic biomarker. IL13 rs20541 gene polymorphism does not seem to play an obvious role in asthma and AC, which requires further evaluation.

IL-13 rs20541 (R130Q) Single Nucleotide Polymorphism in a Sample of Egyptian Children Suffering from Idiopathic Nephrotic Syndrome

“…Several studies have evaluated IL-13 gene SNPs in the coding sequence and the promoter region and reported their association with allergic phenotypes; asthma, allergic rhinitis and atopic dermatitis in different ethnic populations [19][20][21][22][23] , however, little data exists concerning their association with INS.…”

Effect of co-administration of pranoprofen/emedastine difumarate eye drops on efficacy, inflammatory factors, tear film stability and immune function in children with allergic conjunctivitis

“…In clinical practice, allergic reactions are often detected by histamine (HA) and eosinophil cationic protein (ECP) indicators. It has been reported that the degranulation of mast cells promotes a large increases in these two indicators [12]. Eosinophils play a critical role in allergic conjunctivitis and the secretion of the toxic protein, ECP [13].…”

Impact of Interleukin-17 Receptor A Gene Variants on Asthma Susceptibility and Clinical Manifestations in Children and Adolescents

“…Previous studies have indicated that various single nucleotide polymorphisms (SNPs) in several interleukin and interleukin receptor genes, including IL-4 and IL-4R [ 25 , 26 , 27 , 28 ], IL-9 [ 29 ], IL-10 [ 30 ], IL-5 and IL5R [ 31 ], IL13 [ 32 ], IL-33 and its receptor IL-1R-like 1 [ 33 , 34 ], IL-18 [ 35 ], IL-13R [ 36 ], and IL-23R [ 37 ], may be linked to an increased risk of developing asthma.…”