Association of interleukin‐17 gene polymorphisms and susceptibility to brucellosis in Hamadan, western Iran

Abstract

IL‐17is one of the most important inflammatory cytokines that stimulate immunity responses in humans infected with Brucella species, acting as a regulator that reduces release of γ‐IFN, thus increasing resistance to brucellosis. Gene polymorphisms in the regulatory regions of cytokine‐encoding genes affect the amountsof cytokines produced and play a fundamental role in infectious diseases. The aim of this study was to determine the association between IL‐17 gene polymorphisms and susceptibility to brucellosis. In this case‐control study, 86 patients with brucellosis and 86 healthy persons in Hamadan, western Iran, from September 2014 to September 2016, were included. IL‐17 genetic variants at positions rs4711998 A/G, rs8193036 C/T, rs3819024 A/G, rs2275913 A/G, rs3819025 A/G, rs8193038 A/G, rs3804513 A/T, rs1974226 A/G and rs3748067 A/G were analyzed by restriction fragment length polymorphism‐PCR. Serum IL‐17 titers were measured by sandwich ELISA. GG genotypes at positions rs4711998 and rs3748067 were present significantly more frequently in patients with brucellosis than in controls (P < 0.05). The AA genotype at positions rs4711998, rs2275913 and rs3748067 and GG genotype at position rs19744226 were present significantly more frequently in controls than in the patient group. These results suggest that the AA genotype at positions rs3748067, rs3819025 and rs4711998 and GG genotype at position rs3819024 are likely protective factors against brucellosis, whereas the GG genotype at positions rs3748067, rs3819025 and rs4711998 and AA genotype at position rs3819024 may be risk factors against the disease. No significant relationships were found between serum IL‐17 titers and genotypes of the single‐nucleotide polymorphisms.

Vitamin D Receptor Gene Polymorphisms in Childhood Brucellosis in Turkish Children

“…1,11 In recent years, the roles of innate immunity and potential genetic susceptibility to brucellosis caused by polymorphic gene sequences of certain cytokines have been underlined in many studies. [11][12][13][14] In a comprehensive meta-analysis including a total of eight studies with 1,308 cases and 902 controls provided data for the interleukin-6 (IL-6), IL-10, and transforming growth factor β1 (TGF-β1) gene polymorphisms and the susceptibility to human brucellosis was investigated. A nonsignificant trend of increasing risk for brucellosis in persons with the G allele compared with individuals with the C allele in IL-6 polymorphism was found.…”

IL-17A polymorphism (rs2275913) and levels are associated with preeclampsia pathogenesis in Chinese patients

“…Amplicon with mutant allele has a restriction site for TaqI, thus produces two fragments (92bp + 26bp); on the other hand, for wildtype allele, the amplicon remained undigested (118bp). Similarly, the PCR-RFLP technique was also used for genotyping of IL-17A (rs2275913, rs1974226, and rs3748067) polymorphisms, as described in an earlier report (Keramat et al 2019). Following primers were used for ampli cation of IL-17A gene bordering SNPs sites and yield different amplicons (rs2275913: forward-GCTCAGCTTCTAACAAGTAAG, reverse-AAGAGCATCGCACGTTAGTG, amplicon size-338bp; rs1974226: forward-AAAGGAGCTGATGGGGCAGTA, reverse-GGTCTTTCAAGAAGCAGGGAG, amplicon size-211; rs3748067: forward-GGGCTGAACTTTTCTCATACTTAGA, reverse-GAGACATTGTCTTCAGACTACAATG, amplicon size-212bp).…”

IL-17A polymorphism (rs2275913) and levels are associated with preeclampsia pathogenesis in Chinese patients

“…Amplicon with mutant allele has a restriction site for TaqI, thus produces two fragments (92bp + 26bp); on the other hand, for wildtype allele, the amplicon remained undigested (118bp). Similarly, the PCR-RFLP technique was also used for genotyping of IL-17A (rs2275913, rs1974226, and rs3748067) polymorphisms, as described in an earlier report [6]. Following primers were used for ampli cation of IL-17A gene bordering SNPs sites and yield different amplicons (rs2275913: forward-GCTCAGCTTCTAACAAGTAAG, reverse-AAGAGCATCGCACGTTAGTG, amplicon size-338bp; rs1974226: forward-AAAGGAGCTGATGGGGCAGTA, reverse-GGTCTTTCAAGAAGCAGGGAG, amplicon size-211; rs3748067: forward-GGGCTGAACTTTTCTCATACTTAGA, reverse-GAGACATTGTCTTCAGACTACAATG, amplicon size-212bp).…”