Association of interleukin-17A gene polymorphisms and susceptibility to systemic lupus erythematosus in Egyptian children and adolescents: a multi-centre study

Elkoumi, Mohamed A.; Allah, Mayy A.N.; Mohamed, Faisal Y; Boraey, Naglaa F; Abdellatif, Sawsan H.; Shehab, Mona; Sherif, Ahmed H.; Akeel, Nagwa E.; Saleh, Rabab; Elshreif, Anas M.; Abdelrahman, Hind M.; Soliman, Attia A.; Emam, Ahmed A.; Youssef, Manal A.; Fahmy, Dalia S.; Sallam, Mohammad M.; Nawara, Abdallah; Elgohary, Elsayed A.; Ismael, Ali; El-Kaffas, Sameh MH; Sobeih, Alaa A.; Ibrahim, Lamya; Ibrahim, Mohamed; Abdou, Adel M.; Yousry, Sherif M.; Osman, Sherif; Eldeeb, Fatma; Elhewala, Ahmed; Hafez, Sahbaa F. M.; Waked, Nevin M; Elbasyouni, H A A; Fouad, Rania A; Zeid, Ahmed; Nashat, Mohamed; Farghaly, Mohsen A.A.

doi:10.1177/0961203320922305

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…Other studies were performed on Egyptian population regarding A allele frequency of IL-17A rs2275913 polymorphism in Egyptian population. The targeted patients in these studies were those with systemic lupus erythematosus [ 41 ], acute myeloid leukemia [ 42 ] and vitiligo [ 43 ]. Similar to our study and Aziz et al, 2018 study [ 27 ], their reported frequencies were 65% within healthy control group in the study concerned about acute myeloid leukemia patients and 53.8% within healthy control group in vitiligo concerned one.…”

Section: Discussionmentioning

confidence: 99%

IL-23/Th17 pathway and IL-17A gene polymorphism in Egyptian children with immune thrombocytopenic purpura

et al. 2021

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Background Immune thrombocytopenic purpura (ITP) is an acquired complex autoimmune thrombocytopenia. Uncontrolled cellular immune response is one of the key triggers for the loss of immune tolerance in ITP patients. The purpose of this study was to investigate the association of IL-23/Th17, IL-17A and IL-17A rs2275913 gene polymorphism with ITP in Egyptian children. Methods 60 patients with ITP and 50 healthy control children from Minia city- Egypt were involved. Serum levels of IL-23 and IL-17A were determined by enzyme-linked immunosorbent assay. The frequency of Th17 cells was measured using flow cytometer. Genotyping for IL-17A was performed via polymerase chain reaction-restriction fragment length polymorphism. Results Comparing children with ITP to controls, serum levels of IL-23 and IL-17A as well as Th17 cells percentage were significantly increased (p < 0.001). Also, higher levels of these ILs and Th17 cells percentage were associated with decreased platelet count within ITP patients (p < 0.001). Analysis of genotype frequencies for IL-17A rs2275913 polymorphism and its alleles (A, G) showed no significant difference between cases and controls. Likewise, no significant differences were demonstrated between acute and chronic ITP regarding both IL-17A rs2275913 polymorphism prevalence and levels of IL-23, IL-17A plus Th17 cells percentage. The frequency of A alleles was 85 and 86% within patients and controls, respectively. Conclusions Elevated levels of IL-23, IL-17A and Th17 cells may be involved in ITP pathogenesis while IL-17A polymorphism rs2275913 is not prevalent in Egyptian children with ITP.

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Section: Discussionmentioning

confidence: 99%

IL-23/Th17 pathway and IL-17A gene polymorphism in Egyptian children with immune thrombocytopenic purpura

et al. 2021

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“…Based on the inclusion and exclusion criteria, six studies were found suitable for the present analysis. However, one of the studies 25 had included juvenile onset systemic lupus erythematosus patients, thus not included in the meta-analysis. In addition, another independent study in the Indonesian population 37 was excluded from the statistical analysis as the IL-17A rs2275913 genotypes distribution deviated from the HWE (χ 2 =6.80, p = 0.009).…”

Section: Literature Search and Eligible Studiesmentioning

confidence: 99%

“…The rs2275913 polymorphism (G>A) is situated in the IL-17A promoter region (À197) and linked with plasma IL-17A levels. [23][24][25][26] Since the promoter polymorphism in the IL-17A gene (rs2275913) affects the levels of IL-17A and pro-inflammatory cytokines, IL-17A is believed to play a critical role in the pathogenesis of SLE; various population-based genetic association studies have been conducted in various cohorts to decipher the association of IL-17A rs2275913 with susceptibility to develop SLE. The findings of the case-control studies, on the other hand, remained equivocal.…”

Section: Introductionmentioning

confidence: 99%

Interleukin 17A rs2275913 polymorphism is associated with susceptibility to systemic lupus erythematosus: A meta and trial sequential analysis

Padhi

Sarangi

Nayak

et al. 2022

Lupus

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Background The role of cytokines in the development of systemic lupus erythematosus (SLE) has received much attention. Interleukin-17 A upregulates several inflammation-related genes and is thought to have a crucial role in SLE development. The susceptibility to SLE development has been linked to functional genetic variations of the IL-17A gene; nevertheless, the findings have been conflicting. We conducted a meta-analysis that included previously published reports to establish a definitive conclusion on the role of the IL-17A rs2275913 polymorphism in SLE propensity. Materials and Methods The PubMed, Google Scholar, and Scopus databases were used to find eligible published articles. All analyses were conducted using Comprehensive Meta-analysis V3.1. Funnel plots and Egger’s regression analysis were used to assess publication bias. Q statistics and I2 test explored the heterogeneity among the included studies. Combined odds ratio, 95% confidence interval were calculated for each comparison model. Results Based on the inclusion and exclusion criteria, a total of four reports, comprising of 608 SLE patients and 815 healthy controls, were considered for the present meta-analysis. The homozygous comparison (AA vs. GG: combined odds ratio= 2.046, p = 0.005) and recessive genetic model (AA vs. GG+GA: combined odds ratio=1.901, p = 0.010) analysis revealed a significant association of rs2275913 with susceptibility to the development of SLE. However, other genetic comparisons (A vs. G, GA vs. GG, AA+GA vs. GG) failed to demonstrate such association. Furthermore, trial sequential analysis revealed a sufficient number of studies, including enough cases and controls that have already been considered to conclude the role of IL17-A rs2275913 polymorphism in SLE. Conclusions IL-17A rs2275913 polymorphism is associated with susceptibility to SLE development.

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“…9 The SNP rs 2275913 (G 197 A) is located at IL-17A gene promoter region which might affect gene transcription, increasing IL-17A secretion. Many studies linked this SNP to susceptibility to RA, [10][11][12] systemic lupus erythematosus (SLE), 13 inflammatory bowel disease 14 and other autoimmune diseases. IL-23R gene SNP rs 11209026 GA result in missense variant and were linked to several autoimmune conditions such as psoriasis 15 , Ankylosing spondylitis 16 inflammatory bowel disease 17 , and SLE 18 .…”

Section: Introductionmentioning

confidence: 99%

Association of IL-17A rs2275913, IL-23R rs11209026 polymorphisms and serum level of IL-17A with rheumatoid arthritis in Egyptian patients

Abd-Ellah

Mohamed

Esmail

et al. 2023

EJI

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Several studies have reported genetic polymorphisms at the IL-23/IL-17 axis linked to rheumatoid arthritis (RA) in many populations. We aimed to investigate the association of IL-17A rs2275913 and IL-23R rs11209026 polymorphisms with susceptibility to RA and, disease clinical features and the serum level of IL-17A in Egyptian patients. This case-control study included 94 RA cases and 74 controls. TaqMan genotyping assays were used for detection of gene polymorphism and the enzyme-linked immunosorbent assay was used to quantify IL-17A serum level. There was significant difference between RA patients and controls in genotypic distribution and allelic frequency of IL-17A rs 2275913 (p <0.0001). The GG genotype had 7 times higher risk for RA development (OR=7.04: 95% CI 2.11:23.46, p value= 0.001). Also, GG genotype was associated with higher level of serum IL-17 A compared to GA and AA genotypes (p<0.0001). Moreover, patients carrying the GG genotype had higher disease activity score 28 (DAS28) score (4.99±0.84) compared to patients with GA (2.73±0.52, p<0.0001) and patients with AA genotypes (2.67±0.41, p<0.0001). Genotypic distribution of IL-23R rs11209026 was significantly different between RA patients and controls (p <0.0001), but there was no difference between the allelic frequency in both groups (p=0.08). IL-23R rs11209026 SNP was not a risk for RA development. However, DAS28 was lower in AA genotype than AG and GG genotypes (p=0.002, p=0.009 respectively). The mean serum IL-17A level was higher among the RA patients (39.07±10.47 pg./ mL) compared to controls (15.23±1.88 pg/ mL; p <0.0001). Also, there was a positive correlation between IL-17A serum level and DAS28 score (Spearman r = 0.42; p value <0.0001). We concluded that the variant IL-17A (rs2275913) genotype could be a risk factor for RA in our population and IL-17A may play a crucial role in the development and pathogenesis of RA.

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Association of interleukin-17A gene polymorphisms and susceptibility to systemic lupus erythematosus in Egyptian children and adolescents: a multi-centre study

Cited by 4 publications

References 40 publications

IL-23/Th17 pathway and IL-17A gene polymorphism in Egyptian children with immune thrombocytopenic purpura

IL-23/Th17 pathway and IL-17A gene polymorphism in Egyptian children with immune thrombocytopenic purpura

Interleukin 17A rs2275913 polymorphism is associated with susceptibility to systemic lupus erythematosus: A meta and trial sequential analysis

Association of IL-17A rs2275913, IL-23R rs11209026 polymorphisms and serum level of IL-17A with rheumatoid arthritis in Egyptian patients

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