Association of interleukin‐18 gene polymorphism and its protein expression with the lower extremity deep venous thrombosis in the chinese han population: A case‐control study

Chen, Ye-Long; Shou, Lihong; Zhang, Zongxin

doi:10.1002/jcla.22345

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…Thrombosis is one of the most feared complications and a major cause of death in patients with PNH . Thrombus formation mechanism involves many aspects: vascular endothelial factor and high coagulation, deletion of GPI‐anchored protein‐urokinase type plasminogen activator receptor, role of free hemoglobin and depletion of nitric oxide and platelet dysfunction and increased platelet particle release .…”

Section: Discussionmentioning

confidence: 99%

Analysis of clinical characteristics of 92 patients with paroxysmal nocturnal hemoglobinuria: A single institution experience in China

et al. 2019

Clinical Laboratory Analysis

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Objectives We performed a retrospective analysis to investigate the clinical characteristics and therapeutic strategies of Chinese paroxysmal nocturnal hemoglobinuria (PNH) patients, and assessed the efficacy and safety of glucocorticoid in PNH patients. Methods The clinical data of 92 PNH cases in our hospital were analyzed, including clinical manifestation, laboratory examination, treatment efficacy, and survival. Results The main clinical manifestations of these patients included hemoglobinuria, anemia, fatigue, dyspnea, headache, abdominal pain, and erectile dysfunction. Glucocorticoid is still the first‐line treatment for PNH patients to control hemolytic attack, and the short‐term remission rate (12 months) is 79.01% (64/81). Meanwhile, the overall survival (OS) of 10 years after diagnosis was estimated as 70.77% (46/65). Moreover, Cox proportional risk model for multivariate analysis showed that the increase in LDH multiple, thrombosis complications, and complicated with bone marrow failure were the independent adverse prognostic factors affecting the survival of PNH patients. Conclusion Paroxysmal nocturnal hemoglobinuria patients in mainland China have various clinical features, while lower incidences of thrombosis and renal damage. Thrombosis and bone marrow failure are two complications with worse prognosis.

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Section: Discussionmentioning

confidence: 99%

Analysis of clinical characteristics of 92 patients with paroxysmal nocturnal hemoglobinuria: A single institution experience in China

et al. 2019

Clinical Laboratory Analysis

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“…Single-nucleotide polymorphisms in the genes coding for IL-1β, IL-1RA, and IL-18 have been described to predispose to DVT. 85 , 86 , 87 Small reports from patients with rare, monogenic autoinflammatory disorders involving genes coding for NLRP3 and IL-1RA resulting in unchecked IL-1β signaling noted high rates of venous thrombosis. 88 , 89 …”

Section: Nlrp3 Inflammasome and Il-1 In Venous Thrombus Formation And...mentioning

confidence: 99%

“…In patients with altitude-induced DVT, inflammasome pathway component transcripts are highly expressed in peripheral blood mononuclear cells (PBMCs), accompanied by increases in caspase-1 activity and plasma levels of NLRP3, IL-1β, and IL-18. 12 , 87 Similarly, PBMCs from patients with cancer-associated DVT exhibit enhanced NF-kB activity and IL-1β production corresponding to higher circulating levels of IL-1β compared to cancer patients without DVT. 90 …”

Section: Nlrp3 Inflammasome and Il-1 In Venous Thrombus Formation And...mentioning

confidence: 99%

“…A role for IL-1α and IL-18 in VTE might be hypothesized also, although limited evidence is available to date. 16 , 84 , 87 Increased IL-18 levels have been found in rodents and patients with DVT. 84 , 87 IL-18 overexpression by means of lentiviral vectors resulted in larger venous thrombi compared with control rats, whereas IL-18 down-regulation curtailed thrombosis.…”

Section: Nlrp3 Inflammasome and Il-1 In Venous Thrombus Formation And...mentioning

confidence: 99%

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Inflammasome Signaling, Thromboinflammation, and Venous Thromboembolism

Potere

Abbate

Kanthi

et al. 2023

JACC: Basic to Translational Science

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“…Structural disorder and dysfunction of venous endothelial cells modulates the adherence, activation, recruitment and interaction between platelets and leukocytes and disrupts the balance of coagulation/anticoagulation and fibrinolysis/antifibrinolysis (4). The endothelial cell injury caused by endoplasmic reticulum stress (ERS) serves an important role in the formation of DVT (5). Endothelial cells protect the vessels as the first barrier by regulating blood flow, participating in material exchange, preventing lipid leakage and inhibiting platelet aggregation and thrombogenesis (6).…”

Section: Introductionmentioning

confidence: 99%

The X box binding protein 1/C/EBP homologous protein pathway induces apoptosis of endothelial cells under hyperglycemia

Tang

Zheng

et al. 2022

Exp Ther Med

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Venous endothelial cell apoptosis can be induced by endoplasmic reticulum (ER) stress, thus serving an important role in the formation of deep venous thrombosis. X-box binding protein 1 (XBP1) is a protein associated with ER. The present study aimed to explore the function of XBP1/C/EBP homologous protein (CHOP) pathway in the process of endothelial cell apoptosis under hyperglycemia. Small interfering (si)RNAs targeting XBP1 and CHOP were designed to downregulate the expression of XBP1 and CHOP in human umbilical vein endothelial cell, respectively. Flow cytometry was used to determine cell apoptosis. The expression of XBP1, glucose-regulated protein 78 (GRP78), CHOP, Puma, cleaved caspase-3 and Cytochrome c was evaluated by western blotting. There were seven groups of cells that were used in the present study: i) Control (5.5 mM D-glucose); ii) hypertonic (hypertonic control, 27.8 mM mannitol and 5.5 mM D-glucose); iii) 16.7 mM D-glucose; iv) 33.3 mM D-glucose; v) 33.3 mM + NC (33.3 mM D-glucose incubated with NC); vi) 33.3 mM + si-XBP1 (33.3 mM D-glucose incubated with siRNA against XBP1); and vii) 33.3 mM + si-CHOP (33.3 mM D-glucose incubated with siRNA against CHOP). Compared with the control, the apoptosis rate of human umbilical vein endothelial cells (HUVECs) increased greatly with the increase in the concentration of D-glucose. Compared with the 33.3 mM D-glucose group, the HUVECs incubated with 33.3 mM D-glucose and si-XBP1 or 33.3 mM D-glucose and si-CHOP demonstrated a significantly lower apoptosis rate. Compared with the control, XBP1, GRP78, CHOP, Puma, cleaved caspase-3 and cytochrome c were significantly upregulated in the hypertonic, 16.7 mM D-glucose, 33.3 mM D-glucose and 33.3 mM + negative control (NC) groups. Compared with the 33.3 mM D-glucose group, the expression levels of XBP1, GRP78, CHOP, Puma, cleaved caspase-3 and cytochrome c in the 33.3 mM + si-XBP1 or 33.3 mM + si-CHOP groups significantly decreased. High dosage of glucose induced endothelial cell apoptosis by promoting the expression of apoptotic proteins by activating endoplasmic reticulum stress. XBP1/CHOP may be a potential target for the treatment of deep vein thrombosis as one of the key pathways regulating ERS by regulating apoptosis of endothelial cells.

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Association of interleukin‐18 gene polymorphism and its protein expression with the lower extremity deep venous thrombosis in the chinese han population: A case‐control study

Abstract: On the basis of our results, we concluded that the GG genotype of -137G/C polymorphism and IL-18 level are independent risk factors of LEDVT, and IL-18 gene polymorphism affects the level of IL-18 in LEDVT patients.

Cited by 6 publications

References 39 publications

Analysis of clinical characteristics of 92 patients with paroxysmal nocturnal hemoglobinuria: A single institution experience in China

Analysis of clinical characteristics of 92 patients with paroxysmal nocturnal hemoglobinuria: A single institution experience in China

Inflammasome Signaling, Thromboinflammation, and Venous Thromboembolism

The X box binding protein 1/C/EBP homologous protein pathway induces apoptosis of endothelial cells under hyperglycemia

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