Association of interleukin-6 (IL-6) haplotypes with plaque-induced gingivitis in children

Abstract

Complex analysis revealed significant differences in haplotype frequencies between patients and healthy subjects (p<0.01). The CGA haplotype was significantly more frequent in children with gingivitis than in healthy subjects (41.5% versus 34.1%). In subanalyses, we found that IL-6 -174C allele was more frequent in patients (44.3%) than in healthy children (36.1%, p=0.016, P(corr)<0.05). Multivariate logistic regression analysis showed that allele C remained a risk factor for gingivitis in children (p=0.03) regardless of plaque or gender. However, the proportions of the IL-6 -597 and -572 genotypes were comparable between the two groups. CONCLUSIONS. Our results indicate that the three promoter polymorphisms in the IL-6 gene act in a cooperative fashion and suggest that IL-6 haplotypes could play a role in the pathogenesis of gingivitis in Caucasian children.

<b><i>ACE</i></b> Insertion/Deletion Polymorphism Associated with Caries in Permanent but Not Primary Dentition in Czech Children

“…using the following clinical parameters: the cavitation of the lesion as the detection threshold of caries according to the criteria given in the WHO Oral Health Surveys [1997], the gingival index (GI), the plaque index (PI) and the calculus index (CSI) as described previously [Izakovicova Holla et al, 2008]. A radiographic examination was not performed as it was not part of routine dental care for these subjects and would therefore be deemed unethical.…”

Vitamin D Receptor<b><i> Taq</i></b>I Gene Polymorphism and Dental Caries in Czech Children

“…The children underwent dental examination at the Clinic of Stomatology at St. Anne's University Hospital and the Faculty of Medicine, Masaryk University, as described previously [Volckova et al, 2014]. The DMFT score was calculated according to guidelines of the World Health Organization (1997), the gingival index (GI), the plaque index, and the calculus index, as published in our previous study [Izakovicova Holla et al, 2008]. …”

Association study of interleukin‐1 family, interleukin‐6, and its receptor gene polymorphisms in patients with recurrent aphthous stomatitis

“…Single nucleotide analysis of the IL‐6 gene (‐597G>A rs1800797, ‐572G>C rs1800796, ‐174G>C rs1800795) was also based on the PCR‐RFLP approach . Amplification of DNA fragment containing polymorphism IL‐6R (+48892A>C rs2228145) was carried out in reaction volume of 25.0 μL containing 75 ng of genomic DNA, 0.24 μmol/L of each primer, 1.5 U of DNA polymerase (Thermo Fischer Scientific, Waltham, MA, USA), 3.5 mM of MgCl 2 , 10× MgCl 2 ‐free reaction buffer with (NH 4 ) 2 SO 4 , and 0.2 mM deoxyribonucleoside triphosphate mix (Thermo Fischer Scientific, Waltham, MA, USA).…”