Association of Iron Overload with Oxidative Stress, Hepatic Damage and Dyslipidemia in Transfusion-Dependent β-Thalassemia/HbE Patients

Sengsuk, Chintana; Tangvarasittichai, Orathai; Chantanaskulwong, Prasert; Pimanprom, Ampai; Wantaneeyawong, Somsak; Choowet, Anuchit; Tangvarasittichai, Surapon

doi:10.1007/s12291-013-0376-2

Cited by 45 publications

(46 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12,13 In thalassemia, iron mediated ROS formation, originating from imbalanced globin production and regular blood transfusions, can cause systemic tissue damage. 7,14 Antioxidant status can be investigated individually by direct measurement inside the RBC of cytoprotective enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). [15][16][17][18] In our experiments, we chose to measure TAC in plasma, as some studies suggest that assessing total plasmatic antioxidant capacity is more useful than measuring antioxidant individually, since their synergistic interactions could be determined.…”

Section: Discussionmentioning

confidence: 99%

Total antioxidant capacity in Mediterranean β-thalassemic patients

Tsamesidis¹,

Fozza²,

Vagdatli³

et al. 2017

Adv Clin Exp Med

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Total antioxidant capacity in Mediterranean β-thalassemic patients

Tsamesidis¹,

Fozza²,

Vagdatli³

et al. 2017

Adv Clin Exp Med

View full text Add to dashboard Cite

show abstract

“…Results reported by Gavin et al and Shengjiang Guan et al indicated dysfunction of heart and kidney in iron overload (2,33). In the current study, after injection of iron dextran, kidney histological damage was observed, yet treatJundishapur J Nat Pharm Prod.…”

mentioning

confidence: 39%

“…Serum iron concentration, liver biomarkers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatinine phosphokinase (CPK) 2 Jundishapur J Nat Pharm Prod. In Press(In Press):e65028.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

“…However, an excess of iron in most organs is toxic, mostly by increasing oxidative stress and creating reactive oxygen species, which in turn cause peroxidation of lipids, proteins, and nucleic acids. Liver is the target organ for iron toxicity because it is predominantly responsible for taking out and storing excessive amounts of iron (1,2). When the amount of iron in circulation is more than the transferrin binding capacity, iron toxicity occurs.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that toxic level of iron causes several damages on the kidney and heart through increasing the activity of oxidative stress pathway (2,32).…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Protective Effects of Hydro-Alcoholic Extract of Coriandrum sativum in Rats with Experimental Iron-Overload Condition

Talaei

Kheirollah

Babaahmadi‐Rezaei

et al. 2017

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

Background: Development of a new iron-chelating medication may eventually serve as treatment of iron overload diseases, such as thalassemia and other iron related disorders. Coriander sativum, a plant rich of phenol and flavonoids, has an iron-chelating effect in-vitro. The ethanolic extract of C. sativum was investigated for its in-vivo ameliorating effect on hepatic damage in rats with experimental iron overload. Methods: Twenty-four adult male wistar rats were randomly divided to 4 groups: healthy control, iron overload, iron overload treated with deferoxamine, and 200 mg/kg of C. sativum extract, respectively. Assessments were performed for serum iron, ferritin, and serum markers of hepatic and cardiac damages, antioxidant enzyme, and lipid peroxidation levels. Results:The results indicated serum iron and ferritin concentration were significantly increased in the iron overload group compared to healthy controls (P < 0.05). Treatment with C. sativum extract significantly decreased iron and ferritin concentration (P < 0.05). Histopathological changes in the liver, kidney, and the cardiac system, and iron accumulation in the liver were observed in the iron overload group compared to healthy controls (P < 0.05). Treatment with C. sativum extract significantly decreased biochemical parameters, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and Creatinine phosphokinase (CPK), and improved tissue damage and decreased iron accumulation in the liver compared to the iron overload group (P < 0.05). Conclusions:The study showed that the hydro-alcoholic extract of C. sativum has iron-chelating and liver protective effects similar to deferoxamine against experimental iron overload condition in rats.

show abstract

Amlodipine: Can act as an antioxidant in patients with transfusion‐dependent β‐thalassemia? A double‐blind, controlled, crossover trial

Darvishi‐Khezri

Arjmandi

Aliasgharian

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background and Aim: This study aimed to assess the antioxidant effects of amlodipine in transfusion-dependent β-thalassemia (TDT) patients.Methods: This crossover trial consisted of two sequences (AP and PA). In the AP sequence, nine cases received amlodipine 5 mg daily (phase I) and then were switched to placebo (phase II). In PA sequence, 10 patients took the placebo (phase I) and were shifted to amlodipine (phase II). The washout period was 2 weeks. The length of each phase was 6 months. Serum malondialdehyde (MDA, μmol/L), carbonyl (protein CO, μM/L), glutathione (GSH, nM/L), and total antioxidant capacity (TAC, μmol FeSO4/L) were measured in the beginning and at the end of phases I and II. The clinical significance was viewed as a minimum change difference of 5% for each outcome between amlodipine and placebo.Results: Seventeen cases completed the study. According to the baseline MDA values, the adjusted Hedges's g for MDA was −0.59, 95% confidence interval [CI] −1.26 to 0.08. After controlling the baseline protein CO values, Hedges's g computed for protein CO was −0.11, 95% CI −0.76 to 0.55. The estimated values of the adjusted Hedges's g for GSH and TAC were also 0.26, 95% CI −0.40 to 0.91, and 0.42, 95% CI −0.24 to 1.09, respectively. The change difference for MDA was 8.3% (protein CO 2.2%, GSH 3.1%, and TAC 12.9%). Conclusion: Clinically, amlodipine therapy is an efficacious adjuvant treatment with conventional iron chelators for improving the levels of MDA and TAC in patients with TDT. K E Y W O R D S amlodipine, malondialdehyde, oxidative stress, protein carbonyl, reactive oxygen species, redcell transfusion, β-thalassemia 2of10 | DARVISHI-KHEZRI et al. | INTRODUC TI ONThalassemia is well-known as the most prevailing hemoglobinopathy disorder globally. 1 Increased iron absorption and constant red-cell transfusions bring about iron overload in patients with transfusiondependent β-thalassemia (TDT). 2,3 Iron accumulation produces reactive oxygen species (ROS) through the Haber-Weiss and Fenton reactions in conjunction with the production of highly toxic hydroxyl radicals due to peroxidation. 4 ROS can damage lipids, proteins, deoxyribonucleic acid (DNA), and intracellular organelles, e.g., lysosomes and mitochondria. 5 This injury can lead to cellular dysfunction, apoptosis, and necrosis, leading to toxicity and dysfunction in the target organs. 6,7 Hydroxyl radical production and increased lipid peroxidation would be determining factors to trigger cardiac problems caused by iron surplus. [8][9][10] Various studies have shown that calcium channels are involved in iron absorption by myocardial cells. L-type calcium channel blockers (L-TCC blockers), e.g., amlodipine, are traditionally considered therapeutic options in arrhythmia and hypertension, which can inhibit calcium influx into the cell. According to studies, L-TCC blockers may reduce cellular iron uptake and oxidative stress, preventing iron damage. 11 Recent research has recommended amlodipine prescription to mitigate iron deposition into the tiss...

show abstract

Association of Iron Overload with Oxidative Stress, Hepatic Damage and Dyslipidemia in Transfusion-Dependent β-Thalassemia/HbE Patients

Cited by 45 publications

References 27 publications

Total antioxidant capacity in Mediterranean β-thalassemic patients

Total antioxidant capacity in Mediterranean β-thalassemic patients

The Protective Effects of Hydro-Alcoholic Extract of Coriandrum sativum in Rats with Experimental Iron-Overload Condition

Amlodipine: Can act as an antioxidant in patients with transfusion‐dependent β‐thalassemia? A double‐blind, controlled, crossover trial

Contact Info

Product

Resources

About